FastQCFastQC Report
Thu 26 May 2016
SRR1545363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545363_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4081594
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114440.28038065520480476No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC63960.15670348398199332No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCA207.035405E-445.013
CGGTCTA1500.042.031
ACGTTAG2150.040.8139531
CGTTTTT106350.040.0493661
TCGTTGA8100.039.7222224
CGTATGG3800.039.671052
ACGTACG1250.039.61
TCGAATC403.4611185E-739.37518
CGTATGA1450.038.79310615
ACGTATG1450.038.7931061
TAACGCG700.038.571431
TCGAACA356.2522067E-638.571431
ACGTAGG5150.038.009712
GTATGCG2250.038.01
GGCGATA22000.037.7386368
TAGGGCG18350.037.643055
GCTACGA3950.037.59493610
GCGATAT5450.037.568819
TCGTACT301.14043236E-437.49999645
CGACTAT301.14043236E-437.49999629
Produced by FastQC (version 0.11.5)