##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545363_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4081594
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19461195797524	31.0	31.0	33.0	30.0	34.0
2	31.55702723004787	31.0	31.0	34.0	30.0	34.0
3	31.721518602781167	31.0	31.0	34.0	30.0	34.0
4	35.517934169836586	37.0	35.0	37.0	33.0	37.0
5	35.42796025278359	37.0	35.0	37.0	33.0	37.0
6	35.49017491695646	37.0	35.0	37.0	33.0	37.0
7	35.85236846192934	37.0	35.0	37.0	35.0	37.0
8	35.899278076163384	37.0	35.0	37.0	35.0	37.0
9	37.781816858805655	39.0	37.0	39.0	35.0	39.0
10	37.11232596872692	39.0	37.0	39.0	33.0	39.0
11	36.77015082832835	39.0	35.0	39.0	32.0	39.0
12	36.08439619423196	38.0	35.0	39.0	32.0	39.0
13	35.78569965557574	37.0	35.0	39.0	31.0	39.0
14	36.777813030889405	39.0	35.0	41.0	31.0	41.0
15	37.014840525539775	39.0	35.0	41.0	32.0	41.0
16	37.092482495809236	38.0	35.0	41.0	32.0	41.0
17	37.039141080666035	38.0	35.0	41.0	32.0	41.0
18	36.980505655388555	38.0	35.0	41.0	32.0	41.0
19	36.940020246991736	38.0	35.0	41.0	32.0	41.0
20	36.81657019291973	38.0	35.0	41.0	31.0	41.0
21	36.69584260462947	38.0	35.0	41.0	31.0	41.0
22	36.57296414097041	38.0	35.0	40.0	31.0	41.0
23	36.497611962385285	38.0	35.0	40.0	31.0	41.0
24	36.39048028784833	38.0	35.0	40.0	31.0	41.0
25	36.27678622616556	38.0	35.0	40.0	30.0	41.0
26	36.1069342026669	38.0	35.0	40.0	30.0	41.0
27	35.98899915082196	38.0	35.0	40.0	30.0	41.0
28	35.89297367646071	38.0	35.0	40.0	30.0	41.0
29	36.04693068443358	38.0	35.0	40.0	30.0	41.0
30	36.03659305653625	38.0	35.0	40.0	30.0	41.0
31	35.94016749338616	38.0	35.0	40.0	30.0	41.0
32	35.790735923269196	38.0	34.0	40.0	30.0	41.0
33	35.605390688049816	38.0	34.0	40.0	29.0	41.0
34	35.46881953472099	37.0	34.0	40.0	28.0	41.0
35	35.344365951145555	37.0	34.0	40.0	27.0	41.0
36	35.20216856453631	37.0	34.0	40.0	27.0	41.0
37	35.08388242436656	37.0	34.0	40.0	27.0	41.0
38	35.039072970021024	37.0	34.0	40.0	27.0	41.0
39	34.989071426506406	37.0	34.0	40.0	26.0	41.0
40	34.85420352930742	37.0	34.0	40.0	26.0	41.0
41	34.80599368776022	37.0	34.0	40.0	26.0	41.0
42	34.74521106214876	36.0	33.0	40.0	26.0	41.0
43	34.629424435649405	36.0	33.0	40.0	26.0	41.0
44	34.5290634002304	36.0	33.0	40.0	25.0	41.0
45	34.48354270414941	36.0	33.0	40.0	25.0	41.0
46	34.487383605522744	36.0	33.0	40.0	25.0	41.0
47	34.45823298446636	36.0	33.0	40.0	25.0	41.0
48	34.385116942057444	36.0	33.0	40.0	25.0	41.0
49	34.34229592654243	36.0	33.0	40.0	25.0	41.0
50	34.216471309983305	35.0	33.0	40.0	24.0	41.0
51	33.97539588699905	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	2.0
12	7.0
13	11.0
14	34.0
15	122.0
16	314.0
17	750.0
18	1851.0
19	3500.0
20	6232.0
21	9903.0
22	14968.0
23	21690.0
24	31431.0
25	44683.0
26	59103.0
27	70256.0
28	79611.0
29	92968.0
30	111885.0
31	137349.0
32	170992.0
33	221991.0
34	370718.0
35	518948.0
36	309655.0
37	385845.0
38	546297.0
39	869956.0
40	514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.067742651522906	24.522576228796886	29.17396977749379	13.23571134218641
2	30.544169753287566	26.22073631037286	29.724416490224165	13.510677446115416
3	29.223803249416775	25.885891639393826	31.202441987125617	13.687863124063785
4	26.053473226391453	28.492912327879743	30.28292377928819	15.170690666440612
5	23.384442450670008	32.894869014409565	29.407285486993562	14.313403047926865
6	22.06956399877107	41.890104699291506	25.513071608788135	10.527259693149293
7	88.87694366465651	3.9768286605674157	5.54131547625756	1.60491219851852
8	90.12361347061957	2.9589665214129575	5.051678339393874	1.8657416685736012
9	85.06385005466002	4.962325993227156	7.201916702151169	2.7719072499616573
10	47.07347178577781	28.317490666636612	12.679433574235949	11.929603973349627
11	40.0306350901143	23.131747057644635	21.255935793711966	15.581682058529095
12	35.83911089638019	22.293300117552114	25.571970166557477	16.29561881951022
13	23.50645360611565	33.97001269602023	25.429525817609495	17.094007880254626
14	19.071593107986732	36.70529209911618	26.795707755352442	17.427407037544647
15	18.485915061615636	24.22533941396425	40.34730058893658	16.94144493548354
16	19.778375801218836	20.58526644247321	39.24503515048288	20.391322605825078
17	20.46176077287452	21.271150437794645	27.427348236987804	30.83974055234303
18	23.46362720054959	23.984159129986963	30.50237235746622	22.049841311997227
19	28.659905909308964	25.26860829372054	24.744082826464368	21.32740297050613
20	30.79262660617396	23.964607944837237	24.183713519767032	21.05905192922177
21	25.58907132850548	26.261480196217455	26.10230218880173	22.047146286475332
22	24.98340109280835	24.91171341392603	24.626800215798045	25.478085277467578
23	23.03100210358992	29.12808084292558	24.101417240421267	23.73949981306323
24	22.6927519003605	25.107690769831592	32.86654674619769	19.33301058361023
25	21.30836629022877	26.245702046798385	30.111373154703774	22.334558508269073
26	20.766960163112742	31.09118643353553	25.70743194938056	22.43442145397117
27	21.912003006668474	28.83317155013458	27.528313693130674	21.726511750066273
28	18.890168889899385	27.794141210517264	33.059216570780926	20.256473328802425
29	20.132379653635322	24.483522858961475	32.18553829704767	23.19855919035553
30	21.408155735234814	28.051932651802215	28.804481778442444	21.735429834520534
31	27.772875009126334	26.444986934026264	23.64982896388029	22.13230909296711
32	28.18053436966048	26.43058079760015	24.74729235685862	20.641592475880746
33	26.02595946583614	27.811340373393335	24.428691339706987	21.734008821063536
34	21.32269892595883	26.863646898736132	27.773683516782903	24.039970658522137
35	22.52267128969711	26.28977796419732	28.330598290765813	22.85695245533975
36	27.91289383510462	26.29676053032222	24.637212814405352	21.15313282016781
37	22.943903778768785	30.3403523231365	26.275028824522966	20.440715073571745
38	22.12319500665671	30.20035799739024	24.55891007287839	23.11753692307466
39	22.584534375540535	30.157531591824178	25.049331217166625	22.208602815468662
40	24.06990994204715	26.5459033897051	25.516991645910885	23.867195022336862
41	19.916459108867762	25.171293372148234	26.509986049567893	28.402261469416118
42	22.54856803493929	27.104533179929213	24.22244838658622	26.124450398545275
43	23.44804505298665	26.780027606861438	25.43476886726117	24.33715847289074
44	22.63502935372798	29.182324356611655	25.86166090992882	22.320985379731546
45	20.559369697231034	31.62357647526922	23.095903218203475	24.72115060929627
46	23.04947527853089	29.845619138013234	25.483793831527585	21.62111175192829
47	23.420041287790998	26.27738084679662	26.610216498750244	23.692361366662144
48	24.025540021864007	23.544428965742306	28.314893642042787	24.115137370350897
49	21.93268120249099	24.727545169852757	28.63072613297648	24.709047494679776
50	20.65389159235338	29.497764843833068	26.813690925653066	23.034652638160484
51	20.587324461962655	30.008055676287253	24.66382006637603	24.740799795374063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1059.0
1	2648.0
2	4237.0
3	13003.0
4	21769.0
5	16401.5
6	11034.0
7	11164.5
8	11295.0
9	11885.0
10	12475.0
11	12728.5
12	12982.0
13	13033.5
14	13085.0
15	12945.0
16	12805.0
17	12090.5
18	11376.0
19	11405.0
20	11434.0
21	12373.0
22	13312.0
23	13832.0
24	14352.0
25	16402.5
26	22574.5
27	26696.0
28	30372.0
29	34048.0
30	44390.5
31	54733.0
32	63611.5
33	72490.0
34	84071.5
35	95653.0
36	108817.5
37	121982.0
38	138153.0
39	154324.0
40	199986.5
41	245649.0
42	283419.0
43	321189.0
44	333062.0
45	344935.0
46	341216.5
47	337498.0
48	335945.0
49	334392.0
50	319842.0
51	305292.0
52	286448.5
53	267605.0
54	248306.5
55	229008.0
56	213172.0
57	197336.0
58	190588.5
59	183841.0
60	173809.5
61	163778.0
62	143549.0
63	123320.0
64	109291.5
65	95263.0
66	82419.5
67	69576.0
68	57561.0
69	45546.0
70	39891.0
71	34236.0
72	28825.0
73	23414.0
74	19844.5
75	11863.0
76	7451.0
77	5605.0
78	3759.0
79	2619.5
80	1480.0
81	1104.0
82	728.0
83	468.5
84	209.0
85	152.5
86	96.0
87	80.0
88	64.0
89	45.5
90	27.0
91	21.0
92	15.0
93	14.0
94	13.0
95	8.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4081594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.951136882082537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5305966512897	14.09713489918914
2	8.680395589329471	3.1164593882935776
3	2.808913384529376	1.5126956596680172
4	1.3651094020209078	0.9802106293878063
5	0.8169460994761873	0.7332555628490228
6	0.5626817816873625	0.6060466610474355
7	0.4222851142922169	0.5306348522947818
8	0.3450870919104672	0.4955764498499676
9	0.2796451903120434	0.45179541807367607
>10	3.1880043366607445	13.78907604371144
>50	1.3865528905266435	18.111092864347622
>100	1.6057917831817041	43.90747104195744
>500	0.005924128373818462	0.6956931001831039
>1k	0.001791015554875349	0.5316500026532593
>5k	1.3777042729810378E-4	0.1581817656868515
>10k+	1.3777042729810378E-4	0.28302566080680563
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11444	0.28038065520480476	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6396	0.15670348398199332	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04652594060065749	0.0
2	0.0	0.0	0.0	0.13423677122222347	0.0
3	0.0	0.0	0.0	0.20597345056857688	0.0
4	0.0	0.0	0.0	0.34800129557226905	0.0
5	0.0	0.0	0.0	0.6220118904526026	0.0
6	0.0	0.0	0.0	1.0293282477385062	0.0
7	0.0	0.0	0.0	1.2511288481901923	0.0
8	0.0	0.0	0.0	1.8155896936343987	0.0
9	0.0	0.0	0.0	2.0926138170528477	0.0
10	0.0	0.0	0.0	2.4784924713237033	0.0
11	0.0	0.0	0.0	2.843055923739598	0.0
12	0.0	0.0	0.0	3.163886462004795	0.0
13	0.0	0.0	0.0	3.3084378309062585	0.0
14	0.0	0.0	0.0	3.3724324369351777	0.0
15	0.0	0.0	0.0	3.4540672100164787	0.0
16	2.4500232017197202E-5	0.0	0.0	3.614372228105	0.0
17	2.4500232017197202E-5	0.0	0.0	3.815347631342069	0.0
18	2.4500232017197202E-5	0.0	0.0	4.060839956154385	0.0
19	2.4500232017197202E-5	0.0	0.0	4.199241766819532	0.0
20	2.4500232017197202E-5	0.0	0.0	4.351510708806412	0.0
21	2.4500232017197202E-5	0.0	0.0	4.540505498587072	0.0
22	2.4500232017197202E-5	0.0	0.0	4.753118512032309	0.0
23	2.4500232017197202E-5	0.0	0.0	4.984278201114565	0.0
24	2.4500232017197202E-5	0.0	0.0	5.174498002496084	0.0
25	2.4500232017197202E-5	0.0	0.0	5.330809482765802	0.0
26	2.4500232017197202E-5	0.0	0.0	5.479501390878172	0.0
27	2.4500232017197202E-5	0.0	0.0	5.629761313839642	0.0
28	2.4500232017197202E-5	0.0	0.0	5.789012821951424	0.0
29	2.4500232017197202E-5	0.0	0.0	5.967668513820826	0.0
30	2.4500232017197202E-5	0.0	0.0	6.190939128193544	0.0
31	2.4500232017197202E-5	0.0	0.0	6.385152467393866	0.0
32	2.4500232017197202E-5	0.0	0.0	6.576327777824056	0.0
33	2.4500232017197202E-5	0.0	0.0	6.766131075261283	0.0
34	2.4500232017197202E-5	0.0	0.0	6.963137440911566	0.0
35	4.9000464034394404E-5	0.0	0.0	7.195424140666612	0.0
36	4.9000464034394404E-5	0.0	0.0	7.398433063161108	0.0
37	4.9000464034394404E-5	2.4500232017197202E-5	0.0	7.600143473358693	0.0
38	4.9000464034394404E-5	2.4500232017197202E-5	0.0	7.814103999564876	0.0
39	4.9000464034394404E-5	2.4500232017197202E-5	0.0	8.029118035747798	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTCA	20	7.035405E-4	45.0	13
CGGTCTA	150	0.0	42.0	31
ACGTTAG	215	0.0	40.813953	1
CGTTTTT	10635	0.0	40.049366	1
TCGTTGA	810	0.0	39.72222	24
CGTATGG	380	0.0	39.67105	2
ACGTACG	125	0.0	39.6	1
TCGAATC	40	3.4611185E-7	39.375	18
CGTATGA	145	0.0	38.793106	15
ACGTATG	145	0.0	38.793106	1
TAACGCG	70	0.0	38.57143	1
TCGAACA	35	6.2522067E-6	38.57143	1
ACGTAGG	515	0.0	38.00971	2
GTATGCG	225	0.0	38.0	1
GGCGATA	2200	0.0	37.738636	8
TAGGGCG	1835	0.0	37.64305	5
GCTACGA	395	0.0	37.594936	10
GCGATAT	545	0.0	37.56881	9
TCGTACT	30	1.14043236E-4	37.499996	45
CGACTAT	30	1.14043236E-4	37.499996	29
>>END_MODULE