FastQCFastQC Report
Thu 26 May 2016
SRR1545362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545362_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1277376
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96420.7548286487298963No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG207.0335815E-445.01
CGTTTTT108350.043.359481
GTTAGCG556.184564E-1140.9090921
CTACGGG1900.039.0789453
CGGTTTT2900.038.7931061
TACGGGT1700.038.3823554
ACGGGTA2350.038.297875
TAGGGAC9250.038.189195
TACGGGA3250.038.0769234
TAGGGCG5950.037.8151255
CGGGTAT3050.037.622956
GACACGA2400.037.50000425
TATCGCG301.13999304E-437.5000041
ACACGAC2350.037.34042726
CACGGGA2000.037.1249964
AGGGCGA14750.037.0677956
GGGCGAT28000.036.9642877
GTTTTTT133550.036.5087972
GGACCGA7100.036.4436658
CGTAAGG2100.036.428572
Produced by FastQC (version 0.11.5)