##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545362_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1277376
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14527202640413	31.0	31.0	33.0	30.0	34.0
2	31.50639357557994	31.0	31.0	34.0	30.0	34.0
3	31.511465692168947	31.0	31.0	34.0	30.0	34.0
4	35.408676067187734	37.0	35.0	37.0	33.0	37.0
5	35.36151532516659	37.0	35.0	37.0	33.0	37.0
6	35.43314262989128	37.0	35.0	37.0	33.0	37.0
7	35.864270974247205	37.0	35.0	37.0	35.0	37.0
8	35.91793097725337	37.0	35.0	37.0	35.0	37.0
9	37.80414459015983	39.0	37.0	39.0	35.0	39.0
10	37.04489985720728	39.0	37.0	39.0	33.0	39.0
11	36.76420881557192	39.0	37.0	39.0	32.0	39.0
12	36.375660729495465	38.0	35.0	39.0	32.0	39.0
13	36.276424482689514	39.0	35.0	39.0	32.0	39.0
14	37.33589640012025	39.0	36.0	41.0	32.0	41.0
15	37.46881341124305	39.0	36.0	41.0	32.0	41.0
16	37.4734995803898	39.0	35.0	41.0	32.0	41.0
17	37.381116444962174	39.0	35.0	41.0	32.0	41.0
18	37.20017676862568	39.0	36.0	41.0	32.0	41.0
19	37.076243799789566	38.0	35.0	41.0	32.0	41.0
20	36.869641358535	38.0	35.0	41.0	31.0	41.0
21	36.78646303046245	38.0	35.0	41.0	31.0	41.0
22	36.690562528182774	38.0	35.0	41.0	31.0	41.0
23	36.56689807856105	38.0	35.0	40.0	31.0	41.0
24	36.4627854288792	38.0	35.0	40.0	31.0	41.0
25	36.36091409263991	38.0	35.0	40.0	30.0	41.0
26	36.220670342953056	38.0	35.0	40.0	30.0	41.0
27	36.09558657748384	38.0	35.0	40.0	30.0	41.0
28	35.99944104163535	38.0	35.0	40.0	30.0	41.0
29	36.03981521494063	38.0	35.0	40.0	30.0	41.0
30	35.950328642467056	38.0	35.0	40.0	30.0	41.0
31	35.78081316699233	38.0	35.0	40.0	29.0	41.0
32	35.49073491282128	38.0	34.0	40.0	27.0	41.0
33	35.087547441004055	38.0	34.0	40.0	25.0	41.0
34	34.705896306177664	38.0	34.0	40.0	23.0	41.0
35	34.41054239315597	38.0	33.0	40.0	21.0	41.0
36	34.2405979132221	38.0	33.0	40.0	21.0	41.0
37	34.134975919384736	38.0	33.0	40.0	20.0	41.0
38	34.0432120221454	38.0	33.0	40.0	19.0	41.0
39	33.982035046846036	38.0	33.0	40.0	18.0	41.0
40	33.89585212184979	37.0	33.0	40.0	18.0	41.0
41	33.80550206047397	37.0	33.0	40.0	18.0	41.0
42	33.73658186782905	37.0	33.0	40.0	18.0	41.0
43	33.614484693621925	37.0	33.0	40.0	18.0	41.0
44	33.48970154441605	37.0	33.0	40.0	17.0	41.0
45	33.46244410416354	37.0	32.0	40.0	18.0	41.0
46	33.45782760909865	37.0	32.0	40.0	18.0	41.0
47	33.4388746931209	37.0	32.0	40.0	18.0	41.0
48	33.32747836189188	36.0	32.0	40.0	18.0	41.0
49	33.276296877348564	36.0	32.0	40.0	18.0	41.0
50	33.17452418082068	36.0	32.0	40.0	17.0	41.0
51	32.95878582343805	36.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	4.0
14	8.0
15	33.0
16	131.0
17	391.0
18	859.0
19	1709.0
20	2826.0
21	4482.0
22	6672.0
23	10208.0
24	15477.0
25	23733.0
26	32556.0
27	33765.0
28	32004.0
29	31705.0
30	34965.0
31	40792.0
32	48929.0
33	61223.0
34	91979.0
35	117276.0
36	95368.0
37	121135.0
38	176143.0
39	292898.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.109809484443105	22.944692870384287	26.24881006062428	18.696687584548325
2	36.99051806202716	23.69224096898642	26.537370359236434	12.779870609749988
3	27.620293476627083	23.86650445914124	34.9888364898041	13.524365574427577
4	23.809512625883062	26.475524825893082	35.29782930006513	14.417133248158725
5	21.22209905305877	30.516230146800943	34.69550077659201	13.566170023548274
6	19.529332005611504	39.24036462247608	31.769580765569415	9.460722606343003
7	83.93135615511798	2.965454181071196	11.665007014379478	1.4381826494313343
8	84.94609261486048	2.0560899844681595	11.348185655593968	1.6496317450774087
9	80.1974516508843	3.8371630592715067	13.19392254120948	2.771462748634701
10	37.73642216543915	22.02522984618468	25.1558664011223	15.08248158725387
11	31.404457262387897	26.498227616614056	25.67748258930808	16.419832531689966
12	25.55520066135578	23.21031552181973	33.402224560348714	17.832259256475773
13	21.170039205371012	28.06354589408287	34.2959316599028	16.47048324064332
14	19.828539130216942	29.409586527381133	34.567347437246355	16.194526905155566
15	19.630476852547723	24.53592364346911	39.57284307831054	16.26075642567263
16	20.710816548925298	22.43153151460494	38.92182098301518	17.93583095345458
17	21.04893155969738	23.704531790169845	33.18279034520767	22.063746304925097
18	22.771995089934364	24.33535623027206	34.39637193747181	18.49627674232176
19	25.25975124004209	26.61659527030412	30.002207650683903	18.121445838969887
20	25.79044854451626	24.753322435993788	30.876186807956312	18.580042211533645
21	23.633918282479083	26.092708803046243	31.793770980510043	18.479601933964627
22	22.981095621023098	24.43783193045744	31.628666892128866	20.9524055563906
23	20.79145059872739	27.641743699584147	31.45260283581342	20.114202865875043
24	20.898623428027456	25.54486697730347	35.40241870835212	18.15409088631695
25	20.664001828748933	26.452195751290148	33.35071271105767	19.53308970890325
26	20.24032078260434	27.99418495415602	32.28274212134877	19.482752141890877
27	20.751681572223056	26.720245252768176	32.818841124304825	19.709232050703942
28	19.913400596222257	26.67718823588356	34.5891108021444	18.820300365749787
29	20.409339145247756	25.313611653890476	34.01613933563806	20.26090986522371
30	21.069207500375768	25.886504834911566	32.98566749336139	20.058620171351272
31	23.47672102810762	25.60225023798787	31.027356079963926	19.893672653940577
32	23.45253081316699	25.130188761962025	30.793830477478835	20.623449947392153
33	23.406890375269302	26.192679367703793	30.008000776592013	20.392429480434892
34	20.623841374818376	27.2478894233178	30.97373052257127	21.15453867929255
35	21.01808707851095	27.77803872939526	30.804320732501626	20.399553459592166
36	24.039437096046896	28.24211508592615	27.732320006012323	19.98612781201463
37	21.20808595119996	30.19839107670725	29.019489954406534	19.574033017686258
38	21.368649481436947	30.451957763415	28.019471165890074	20.15992158925798
39	21.867328022446014	29.13198619670324	28.153026203717623	20.847659577133122
40	22.271985695676136	27.611525502279672	28.20900032566762	21.90748847637657
41	20.32353825341951	26.64039405781853	28.885543489152766	24.1505241996092
42	21.635524700636307	28.063311037627138	27.81459805100456	22.486566210732
43	21.575010020542113	27.850609374217143	28.685054361440955	21.88932624379979
44	21.271732050703942	28.955843854902554	28.596904905055364	21.175519189338143
45	20.541641615311388	29.491081717520917	27.65473909013478	22.312537577032916
46	22.130132396412648	29.17660892329275	27.692629264993236	21.00062941530137
47	21.991332231073702	27.59336326970289	28.742907335036826	21.67239716418658
48	22.191195074903554	26.580349090635803	29.33161418407736	21.896841650383287
49	21.678973144947143	26.358174883511197	29.35666553935568	22.606186432185982
50	20.491460619269503	28.457556741319706	29.0327202014129	22.018262437997898
51	20.0215911368305	28.882646926198706	28.194517510897338	22.90124442607345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	321.0
1	754.0
2	1187.0
3	10040.0
4	18893.0
5	14535.5
6	10178.0
7	10098.0
8	10018.0
9	10270.0
10	10522.0
11	10819.5
12	11117.0
13	11006.0
14	10895.0
15	10628.5
16	10362.0
17	10132.5
18	9903.0
19	9439.5
20	8976.0
21	8860.0
22	8744.0
23	9119.0
24	9494.0
25	10159.5
26	11972.0
27	13119.0
28	15226.5
29	17334.0
30	19657.0
31	21980.0
32	25377.0
33	28774.0
34	33496.5
35	38219.0
36	42173.5
37	46128.0
38	51658.5
39	57189.0
40	65189.5
41	73190.0
42	81592.0
43	89994.0
44	95980.0
45	101966.0
46	102843.0
47	103720.0
48	98594.5
49	93469.0
50	89326.0
51	85183.0
52	80747.0
53	76311.0
54	70403.5
55	64496.0
56	59785.5
57	55075.0
58	50955.5
59	46836.0
60	43007.5
61	39179.0
62	34768.5
63	30358.0
64	26267.0
65	22176.0
66	18451.5
67	14727.0
68	12255.0
69	9783.0
70	7960.5
71	6138.0
72	5538.5
73	4939.0
74	3765.5
75	2022.0
76	1452.0
77	1093.0
78	734.0
79	628.5
80	523.0
81	365.5
82	208.0
83	143.0
84	78.0
85	58.5
86	39.0
87	26.0
88	13.0
89	10.5
90	8.0
91	4.5
92	1.0
93	2.5
94	4.0
95	4.5
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1277376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.335646362655574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.69716826326366	24.961042610538893
2	6.844481762710545	4.700194106801495
3	3.4492301461433126	3.552946395641628
4	2.4131818552261413	3.3143263515928756
5	1.8967698116846652	3.2563408742682736
6	1.5532121883829406	3.1998326655889815
7	1.3029477860647813	3.1316288087927737
8	1.113940933132065	3.059830555912732
9	1.0064341803147288	3.1100911292318094
>10	7.662989493541694	45.0651465530578
>50	0.04817802830409245	1.1034375840330044
>100	0.010777618341812732	0.6767308871855237
>500	4.5862192638188135E-4	0.10928473713215248
>1k	0.0	0.0
>5k	2.2931096319094067E-4	0.7591667402220563
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9642	0.7548286487298963	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022702790720978003	0.0
2	0.0	0.0	0.0	0.06646437697279423	0.0
3	0.0	0.0	0.0	0.1297973345357984	0.0
4	0.0	0.0	0.0	0.22475762813768224	0.0
5	0.0	0.0	0.0	0.4348758705345959	0.0
6	0.0	0.0	0.0	0.9823262688511448	0.0
7	0.0	0.0	0.0	1.3335932411443459	0.0
8	0.0	0.0	0.0	2.015224961170399	0.0
9	0.0	0.0	0.0	2.464896788416253	0.0
10	0.0	0.0	0.0	2.8728424520266547	0.0
11	0.0	0.0	0.0	3.172989002455033	0.0
12	0.0	0.0	0.0	3.4206842777694275	0.0
13	0.0	0.0	0.0	3.5763941079212387	0.0
14	0.0	0.0	0.0	3.6621949997494863	0.0
15	0.0	0.0	0.0	3.7368010671877347	0.0
16	0.0	0.0	0.0	3.8557167192745125	0.0
17	0.0	0.0	0.0	3.9863751941480032	0.0
18	0.0	0.0	0.0	4.147251866325968	0.0
19	0.0	0.0	0.0	4.259748108622676	0.0
20	0.0	0.0	0.0	4.3734969186833	0.0
21	0.0	0.0	0.0	4.517463926048399	0.0
22	0.0	0.0	0.0	4.671060048098602	0.0
23	0.0	0.0	0.0	4.849472668971392	0.0
24	0.0	0.0	0.0	4.993909389247959	0.0
25	0.0	0.0	0.0	5.124802720577184	0.0
26	0.0	0.0	0.0	5.24880692920487	0.0
27	0.0	0.0	0.0	5.377508266947242	0.0
28	0.0	0.0	0.0	5.525467834059823	0.0
29	0.0	0.0	0.0	5.674366826995341	0.0
30	0.0	0.0	0.0	5.851761736559948	0.0
31	0.0	0.0	0.0	6.019057818527982	0.0
32	0.0	0.0	0.0	6.191207600581191	0.0
33	0.0	0.0	0.0	6.358816824490205	0.0
34	0.0	0.0	0.0	6.539499724435092	0.0
35	0.0	0.0	0.0	6.733256300415852	0.0
36	0.0	0.0	0.0	6.914330627786963	0.0
37	0.0	0.0	0.0	7.108948344105416	0.0
38	0.0	0.0	0.0	7.3215717220301615	0.0
39	0.0	0.0	0.0	7.575999549075605	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	20	7.0335815E-4	45.0	1
CGTTTTT	10835	0.0	43.35948	1
GTTAGCG	55	6.184564E-11	40.909092	1
CTACGGG	190	0.0	39.078945	3
CGGTTTT	290	0.0	38.793106	1
TACGGGT	170	0.0	38.382355	4
ACGGGTA	235	0.0	38.29787	5
TAGGGAC	925	0.0	38.18919	5
TACGGGA	325	0.0	38.076923	4
TAGGGCG	595	0.0	37.815125	5
CGGGTAT	305	0.0	37.62295	6
GACACGA	240	0.0	37.500004	25
TATCGCG	30	1.13999304E-4	37.500004	1
ACACGAC	235	0.0	37.340427	26
CACGGGA	200	0.0	37.124996	4
AGGGCGA	1475	0.0	37.067795	6
GGGCGAT	2800	0.0	36.964287	7
GTTTTTT	13355	0.0	36.508797	2
GGACCGA	710	0.0	36.443665	8
CGTAAGG	210	0.0	36.42857	2
>>END_MODULE