##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545361_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5303891
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14864294911038	31.0	31.0	33.0	30.0	34.0
2	31.501446579501728	31.0	31.0	34.0	30.0	34.0
3	31.67519675649443	31.0	31.0	34.0	30.0	34.0
4	35.459349560539614	37.0	35.0	37.0	33.0	37.0
5	35.35815818990247	37.0	35.0	37.0	33.0	37.0
6	35.40686356488095	37.0	35.0	37.0	33.0	37.0
7	35.85174355958672	37.0	35.0	37.0	35.0	37.0
8	35.90993404653301	37.0	35.0	37.0	35.0	37.0
9	37.7932218064059	39.0	37.0	39.0	35.0	39.0
10	37.04140337725643	39.0	37.0	39.0	33.0	39.0
11	36.68484099692094	39.0	35.0	39.0	32.0	39.0
12	35.92351671631261	37.0	35.0	39.0	31.0	39.0
13	35.59528391514833	37.0	35.0	39.0	30.0	39.0
14	36.56362131122227	38.0	35.0	41.0	31.0	41.0
15	36.83700852826727	38.0	35.0	41.0	31.0	41.0
16	36.93502374011834	38.0	35.0	41.0	32.0	41.0
17	36.88691264582926	38.0	35.0	41.0	32.0	41.0
18	36.81935601617756	38.0	35.0	41.0	31.0	41.0
19	36.79243898488864	38.0	35.0	40.0	31.0	41.0
20	36.6675327603829	38.0	35.0	40.0	31.0	41.0
21	36.55326023856825	38.0	35.0	40.0	31.0	41.0
22	36.45178266295442	38.0	35.0	40.0	31.0	41.0
23	36.36438380803829	38.0	35.0	40.0	31.0	41.0
24	36.271468059958245	38.0	35.0	40.0	31.0	41.0
25	36.167183865580945	38.0	35.0	40.0	30.0	41.0
26	36.00732556532553	38.0	35.0	40.0	30.0	41.0
27	35.87033971851986	37.0	34.0	40.0	30.0	41.0
28	35.814218655700124	37.0	34.0	40.0	30.0	41.0
29	35.951600061162644	37.0	35.0	40.0	30.0	41.0
30	35.93063337840088	37.0	35.0	40.0	30.0	41.0
31	35.833885726535485	38.0	34.0	40.0	30.0	41.0
32	35.712253136423804	37.0	34.0	40.0	29.0	41.0
33	35.539187928258706	37.0	34.0	40.0	29.0	41.0
34	35.41403528089096	37.0	34.0	40.0	28.0	41.0
35	35.298282902118466	37.0	34.0	40.0	27.0	41.0
36	35.15705941166589	37.0	34.0	40.0	27.0	41.0
37	35.048629770106515	37.0	34.0	40.0	27.0	41.0
38	35.00864817923294	37.0	34.0	40.0	27.0	41.0
39	34.97125544246667	37.0	34.0	40.0	27.0	41.0
40	34.84827817917073	37.0	34.0	40.0	26.0	41.0
41	34.797433431418554	36.0	34.0	40.0	26.0	41.0
42	34.74855252492934	36.0	33.0	40.0	26.0	41.0
43	34.62124956941989	36.0	33.0	40.0	26.0	41.0
44	34.5053537110774	36.0	33.0	40.0	25.0	41.0
45	34.45625730242194	36.0	33.0	40.0	24.0	41.0
46	34.46145216785187	36.0	33.0	40.0	25.0	41.0
47	34.434718586788456	36.0	33.0	40.0	25.0	41.0
48	34.36902681446508	36.0	33.0	40.0	24.0	41.0
49	34.353675443179355	36.0	33.0	40.0	24.0	41.0
50	34.22221761344643	35.0	33.0	40.0	24.0	41.0
51	33.96418365309543	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	10.0
11	5.0
12	9.0
13	17.0
14	51.0
15	155.0
16	432.0
17	1038.0
18	2457.0
19	4949.0
20	8596.0
21	13621.0
22	20543.0
23	30258.0
24	43190.0
25	60470.0
26	78663.0
27	93407.0
28	106739.0
29	124420.0
30	149028.0
31	182523.0
32	225632.0
33	291040.0
34	487670.0
35	700047.0
36	386520.0
37	475756.0
38	680523.0
39	1135557.0
40	561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.13906884587183	23.737195956704237	29.33891363906234	12.784821558361587
2	30.208727894294963	25.441322229284125	30.772351845088824	13.57759803133209
3	29.10042834590681	25.521697184199297	31.195456316881327	14.182418153012572
4	25.599470275690056	28.338365173794106	30.700762892751754	15.361401657764084
5	23.71457105736147	32.53300265786005	29.628945994553806	14.123480290224666
6	20.86471988206394	41.91453029483449	26.66263314988939	10.558116673212176
7	89.29825292412683	3.863352395439499	5.23870117240343	1.5996935080302366
8	90.70135491095122	2.7740577624992673	4.717706302787897	1.806881023761612
9	86.10899055052225	4.512592736162942	6.62066773242512	2.7577489808896902
10	48.59847232908821	21.737343395631623	15.81576619881517	13.848418076464993
11	42.36883827363722	23.773942564053446	18.22993722910218	15.627281933207149
12	35.43157278307567	23.424180474297078	25.22246403630844	15.921782706318814
13	22.194064697030917	36.78942497121453	25.168937295280013	15.847573036474543
14	18.248602771059964	38.52303902927115	27.46110355586116	15.767254643807727
15	17.549625359948006	24.57791836219862	42.23853770750568	15.633918570347694
16	18.16570136905151	20.837984792673907	40.889188710703145	20.107125127571436
17	19.187479531536376	22.077829276657457	27.393078025170574	31.34161316663559
18	23.24687667978094	24.386115778020326	31.595294850516346	20.77171269168239
19	28.78909087686757	26.391549147597495	23.66059181834619	21.15876815718875
20	30.334239523398953	24.657237488477797	24.50746065482869	20.501062333294556
21	23.958938824346127	28.20127713785973	26.64785154898545	21.19193248880869
22	24.27570626922763	25.807543933312356	24.48698512092349	25.429764676536525
23	21.32685230522271	30.331826200802393	25.057585082348034	23.283736411626858
24	21.53705647419979	25.534574522741888	34.38228651380656	18.54608248925176
25	19.67985390348331	26.828813035562003	31.78142235577617	21.70991070517852
26	19.27848818914265	32.57866724636686	26.443096209933426	21.699748354557062
27	20.511186975750444	30.821711833821624	28.183554300041237	20.4835468903867
28	18.12429780325425	28.525680486269422	34.351497796617615	18.998523913858712
29	19.034384379316997	25.62464047620888	33.03267355984503	22.308301584629096
30	21.057804543871658	29.723009013571357	28.594648721099286	20.6245377214577
31	27.044051244642848	27.376750389478214	24.614419866471614	20.964778499407323
32	28.06498097340236	27.20133200324064	25.150102066577162	19.58358495677984
33	26.51347850097221	27.77602330062967	24.549712654351303	21.160785544046814
34	20.305187267234565	27.738220864644465	28.241775707683285	23.714816160437685
35	21.348666479005697	26.439777891363153	29.522967195215738	22.688588434415415
36	28.066772111266992	26.292734899717964	25.208794826288848	20.431698162726196
37	21.356981129514164	30.704514855226094	27.98503966239125	19.953464352868487
38	21.125716950065527	31.541881233984636	24.711763495893862	22.620638320055974
39	21.242216327597983	30.805289927715336	26.129533959125478	21.822959785561206
40	23.515773608469708	26.44980826340511	25.53070943577083	24.503708692354348
41	18.99714756581536	25.325859826304875	26.889674014794046	28.787318593085722
42	22.1267367674034	27.233987274625367	24.849417154311805	25.789858803659428
43	22.407323227419266	27.156459286210822	26.239679510759178	24.196537975610735
44	21.248871818821314	29.82642365765058	26.747495376507548	22.177209147020555
45	19.423193274522422	32.8827836016992	23.811386772465724	23.88263635131265
46	22.192763765318706	30.770221333734042	25.77298439956628	21.26403050138097
47	22.212353157332984	26.93897744127849	27.601000850130593	23.247668551257934
48	23.153303866915817	24.912389790815837	29.11639775402624	22.8179085882421
49	21.450289985220284	24.682935603314622	29.63991907073505	24.22685534073004
50	19.96275941568181	30.776707138212306	26.348354443935595	22.912179002170294
51	19.286689715154402	31.56620300077811	24.780901417468797	24.36620586659869
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1264.0
1	2633.0
2	4002.0
3	12473.5
4	20945.0
5	16267.0
6	11589.0
7	12202.0
8	12815.0
9	13896.5
10	14978.0
11	15157.5
12	15337.0
13	15697.0
14	16057.0
15	15423.5
16	14790.0
17	14306.0
18	13822.0
19	14505.5
20	15189.0
21	15453.0
22	15717.0
23	17465.5
24	19214.0
25	22773.0
26	30104.0
27	33876.0
28	44070.0
29	54264.0
30	65450.0
31	76636.0
32	92881.5
33	109127.0
34	126361.5
35	143596.0
36	156341.0
37	169086.0
38	201316.5
39	233547.0
40	299551.5
41	365556.0
42	420899.0
43	476242.0
44	479768.0
45	483294.0
46	476437.0
47	469580.0
48	459141.5
49	448703.0
50	435818.5
51	422934.0
52	386698.0
53	350462.0
54	319654.0
55	288846.0
56	258602.5
57	228359.0
58	214249.0
59	200139.0
60	182567.0
61	164995.0
62	142529.5
63	120064.0
64	103968.0
65	87872.0
66	72037.5
67	56203.0
68	48653.0
69	41103.0
70	34697.5
71	28292.0
72	24696.5
73	21101.0
74	17069.0
75	9914.0
76	6791.0
77	5585.5
78	4380.0
79	3137.5
80	1895.0
81	1459.5
82	1024.0
83	749.0
84	474.0
85	335.5
86	197.0
87	140.0
88	83.0
89	71.0
90	59.0
91	36.0
92	13.0
93	11.0
94	9.0
95	5.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5303891.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.047423826027934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.01661960043525	13.299823798026178
2	9.637047691436754	3.2857367285513277
3	3.3905703521850588	1.7340146941699046
4	1.565548780112284	1.0675429429958048
5	0.873584433742232	0.7446182044912222
6	0.5428212879131623	0.5552222734107607
7	0.38401439196051196	0.4582519266531682
8	0.2953359809553646	0.4027774110737451
9	0.24009737080127386	0.3683737475607869
>10	2.32388298197347	9.187615524180014
>50	0.9668019351470489	12.090962762047571
>100	1.7448227921200024	54.09551926561703
>500	0.016732900488906462	1.7700319460786669
>1k	0.0017848427188165697	0.42611070206154567
>5k	1.115526699260356E-4	0.10044703567140109
>10k+	2.231053398520712E-4	0.4129510374109191
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11132	0.20988364956972155	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10583	0.1995327581204063	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06069129248696853	0.0
2	0.0	0.0	0.0	0.20192722663418233	0.0
3	0.0	0.0	0.0	0.29923314789085975	0.0
4	0.0	0.0	0.0	0.4722382115318735	0.0
5	0.0	0.0	0.0	0.8001295652569029	0.0
6	0.0	0.0	0.0	1.1941044791455933	0.0
7	0.0	0.0	0.0	1.4180532744734007	0.0
8	0.0	0.0	0.0	1.9822805559164018	0.0
9	0.0	0.0	0.0	2.207228617631848	0.0
10	0.0	0.0	0.0	2.57331834308058	0.0
11	0.0	0.0	0.0	2.9732700012123177	0.0
12	0.0	0.0	0.0	3.287888080656258	0.0
13	1.885408278563794E-5	0.0	0.0	3.4271066279454083	0.0
14	1.885408278563794E-5	0.0	0.0	3.481406386368046	0.0
15	1.885408278563794E-5	0.0	0.0	3.567984334519695	0.0
16	1.885408278563794E-5	0.0	0.0	3.7698738529883062	0.0
17	1.885408278563794E-5	0.0	0.0	4.011979130038683	0.0
18	1.885408278563794E-5	0.0	0.0	4.3140403903473885	0.0
19	1.885408278563794E-5	0.0	0.0	4.466060859847987	0.0
20	1.885408278563794E-5	0.0	0.0	4.626339417608695	0.0
21	1.885408278563794E-5	0.0	0.0	4.850363629267645	0.0
22	1.885408278563794E-5	0.0	0.0	5.08340009249813	0.0
23	1.885408278563794E-5	0.0	0.0	5.3639111361828515	0.0
24	1.885408278563794E-5	0.0	0.0	5.5749448848024965	0.0
25	1.885408278563794E-5	0.0	0.0	5.757150740842902	0.0
26	1.885408278563794E-5	0.0	0.0	5.928854872771707	0.0
27	3.770816557127588E-5	0.0	0.0	6.0932624746624695	0.0
28	3.770816557127588E-5	0.0	0.0	6.272300844794888	0.0
29	3.770816557127588E-5	0.0	0.0	6.463613222820755	0.0
30	3.770816557127588E-5	0.0	0.0	6.695424170670174	0.0
31	3.770816557127588E-5	0.0	0.0	6.914810277963857	0.0
32	3.770816557127588E-5	0.0	0.0	7.118622912876603	0.0
33	3.770816557127588E-5	0.0	0.0	7.322341277375421	0.0
34	3.770816557127588E-5	0.0	0.0	7.541519989758462	0.0
35	3.770816557127588E-5	0.0	0.0	7.797803537063639	0.0
36	3.770816557127588E-5	0.0	0.0	8.020375984348094	0.0
37	3.770816557127588E-5	0.0	0.0	8.246907789017534	0.0
38	3.770816557127588E-5	0.0	0.0	8.477097285747389	0.0
39	3.770816557127588E-5	0.0	0.0	8.706853138573171	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	140	0.0	41.785713	1
TAATGCG	130	0.0	39.80769	1
CGTTTTT	11795	0.0	39.02925	1
CTATGCG	255	0.0	38.82353	1
GCGATAT	630	0.0	38.57143	9
CATACGA	750	0.0	38.399998	18
AGGGCGA	6245	0.0	38.33467	6
GGGCGAT	12475	0.0	38.092186	7
TGGGCGA	3805	0.0	37.90407	6
GGCGATA	3180	0.0	37.5	8
CGAACTA	60	1.5643309E-10	37.499996	22
GACCGAT	3140	0.0	37.476112	9
TAAGGGC	2905	0.0	37.409637	4
GCTACGA	855	0.0	37.105263	10
CACGACC	445	0.0	36.91011	27
AGCTACG	850	0.0	36.794117	9
CGGTCTA	240	0.0	36.5625	31
ATAGGGC	2660	0.0	36.37218	4
ATGGGCG	2075	0.0	36.325302	5
GGCGATT	3745	0.0	36.288383	8
>>END_MODULE