FastQCFastQC Report
Thu 26 May 2016
SRR1545360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545360_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5104824
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC113860.2230439286447486No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101850.19951716258973864No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT54270.10631120681143953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96900.040.239941
TAAACGG2550.039.7058832
AAGCGTA403.4613367E-739.37517
TGCGATT403.4613367E-739.37516
TTGCGTC4400.039.37516
GCGTACC403.4613367E-739.3759
CGTAAGG7100.038.9788742
TCGTTGA9650.038.70466224
CGTAAAT700.038.5714316
CGTTGAT9600.038.43750425
ACTACGG1900.037.8947372
AGGGCGA52750.037.5355456
GTACGAT301.1404723E-437.50000411
TACGTAG2700.037.51
GCGATAT5650.037.03549
ATTATAC34350.036.9432342
GGGCGAT105150.036.8473637
ATGATCG13800.036.68478427
ATTAGCG4850.036.6494871
TATACTA34050.036.6079344
Produced by FastQC (version 0.11.5)