##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545360_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5104824
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138467065661814	31.0	31.0	33.0	30.0	33.0
2	31.491475122354856	31.0	31.0	34.0	30.0	34.0
3	31.68536701755046	31.0	31.0	34.0	30.0	34.0
4	35.47603247438109	37.0	35.0	37.0	33.0	37.0
5	35.37605390509056	37.0	35.0	37.0	33.0	37.0
6	35.429520586801814	37.0	35.0	37.0	33.0	37.0
7	35.818296967730916	37.0	35.0	37.0	35.0	37.0
8	35.86195077440476	37.0	35.0	37.0	35.0	37.0
9	37.763996760711045	39.0	37.0	39.0	35.0	39.0
10	37.05884081410054	39.0	37.0	39.0	33.0	39.0
11	36.69872830091693	39.0	35.0	39.0	32.0	39.0
12	35.90187340445038	37.0	35.0	39.0	31.0	39.0
13	35.53914121231212	37.0	35.0	39.0	30.0	39.0
14	36.45000513240026	38.0	35.0	41.0	31.0	41.0
15	36.71983657027157	38.0	35.0	41.0	31.0	41.0
16	36.816403072858144	38.0	35.0	41.0	32.0	41.0
17	36.7271535708185	38.0	35.0	41.0	31.0	41.0
18	36.67293563891723	38.0	35.0	40.0	31.0	41.0
19	36.65483178264324	38.0	35.0	40.0	31.0	41.0
20	36.554781712356785	38.0	35.0	40.0	31.0	41.0
21	36.443344961550096	38.0	35.0	40.0	31.0	41.0
22	36.32779641374512	38.0	35.0	40.0	30.0	41.0
23	36.25954489322257	38.0	35.0	40.0	30.0	41.0
24	36.18095099850651	38.0	35.0	40.0	30.0	41.0
25	36.05292758379133	37.0	34.0	40.0	30.0	41.0
26	35.89841256035468	37.0	34.0	40.0	30.0	41.0
27	35.76492588187173	37.0	34.0	40.0	30.0	41.0
28	35.692869333007366	37.0	34.0	40.0	29.0	41.0
29	35.82035051551239	37.0	34.0	40.0	30.0	41.0
30	35.789438578097894	37.0	34.0	40.0	30.0	41.0
31	35.66908340032879	37.0	34.0	40.0	29.0	41.0
32	35.56154903675426	37.0	34.0	40.0	29.0	41.0
33	35.39719900235542	37.0	34.0	40.0	29.0	41.0
34	35.23322919654037	37.0	34.0	40.0	27.0	41.0
35	35.13770425777657	37.0	34.0	40.0	27.0	41.0
36	34.99278251316794	37.0	34.0	40.0	26.0	41.0
37	34.906915302075056	37.0	34.0	40.0	26.0	41.0
38	34.85507139913149	36.0	33.0	40.0	27.0	41.0
39	34.80472235673551	36.0	33.0	40.0	26.0	41.0
40	34.689782644808126	36.0	33.0	40.0	26.0	41.0
41	34.644760328661675	36.0	33.0	40.0	26.0	41.0
42	34.584604875701885	36.0	33.0	40.0	26.0	41.0
43	34.45966716972025	36.0	33.0	40.0	26.0	41.0
44	34.34329724198131	35.0	33.0	40.0	25.0	41.0
45	34.29550009951372	35.0	33.0	40.0	24.0	41.0
46	34.29311510054019	35.0	33.0	40.0	24.0	41.0
47	34.254621119161015	35.0	33.0	40.0	25.0	41.0
48	34.19236099031034	35.0	33.0	40.0	24.0	41.0
49	34.16711761267381	35.0	33.0	40.0	24.0	41.0
50	34.044331205150264	35.0	33.0	39.0	24.0	41.0
51	33.81487961191218	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	14.0
12	24.0
13	22.0
14	62.0
15	208.0
16	425.0
17	1104.0
18	2633.0
19	4896.0
20	8570.0
21	13662.0
22	20387.0
23	29613.0
24	42278.0
25	58167.0
26	76093.0
27	92647.0
28	106942.0
29	124047.0
30	148754.0
31	181478.0
32	224357.0
33	289121.0
34	488573.0
35	677350.0
36	379413.0
37	470947.0
38	659846.0
39	1002669.0
40	519.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.36155683330121	24.059673751729736	28.953103965974147	12.62566544899491
2	29.342578705945595	25.594535678409287	31.01121605759572	14.051669558049406
3	29.2839478892906	25.427575955605914	30.933759910233928	14.354716244869559
4	26.060780939754242	27.838354466285224	30.636315767203726	15.464548826756808
5	23.370913473216707	32.64619896787823	28.84826195770902	15.134625601196044
6	21.392569068003127	41.82416475083176	25.671169074585137	11.112097106579972
7	89.60882882544041	3.750726763547578	4.9664591766532995	1.6739852343587165
8	90.87500372196965	2.7468136021927494	4.608268571061411	1.7699141047761884
9	86.82892887198462	3.832139952327446	6.456716235466688	2.8822149402212496
10	50.54340756899748	20.962995002374225	13.728622181685402	14.76497524694289
11	42.655731128046725	22.20411516636029	18.285037838718825	16.855115866874158
12	38.10004027562948	20.7349166200441	24.264009885551392	16.901033218775023
13	24.40936259506694	33.16817582741344	24.578575088974663	17.843886488544953
14	19.534032906913147	34.771012673502554	27.858982013875504	17.835972405708798
15	19.366779344400513	21.81610962493516	41.339897320652	17.477213710012336
16	21.14376519151297	18.821785040973012	39.17004778225459	20.864401985259434
17	21.545914217610637	18.698294005826646	27.266777463826376	32.489014312736344
18	25.15044593114278	22.13888275090385	31.033371571674166	21.677299746279203
19	29.488342791054105	25.177146166057828	23.551448590588038	21.78306245230002
20	31.86742579176089	23.4762452143306	23.159779847454097	21.496549146454413
21	25.39864253890046	26.594452619718133	26.016861697876358	21.990043143505044
22	24.94230947041465	23.91430145290024	25.187293430684388	25.956095646000726
23	23.110708616007134	28.363622330564187	24.157757446681806	24.367911606746873
24	24.411458651659686	23.57609194753825	32.09540622752126	19.917043173280803
25	21.79103530307803	24.043257906638896	30.317656397164722	23.848050393118353
26	20.91964385060092	29.464678899801445	25.323125733619804	24.292551515977827
27	21.07118678332495	28.32181873459301	27.28871749545136	23.318276986630686
28	18.05619547314462	27.084107111234395	32.77840724773273	22.081290167888255
29	20.4452886132803	24.81762348711728	31.53701283335136	23.20007506625106
30	22.86735056879532	26.75373333145276	29.28831630630165	21.090599793450274
31	29.30298870245086	25.326593042189117	23.769340529663708	21.60107772569632
32	29.877562869944196	25.49057518927195	23.805972546751857	20.825889394032
33	28.211687611561143	25.158222888781278	24.090037188353605	22.540052311303974
34	21.275757988914016	26.619899138540333	28.104201045912653	24.000141826633005
35	22.430391331806934	25.929297464515916	28.741402250106958	22.898908953570192
36	28.983212741516652	26.388000839989783	24.517005875227042	20.111780543266526
37	23.624438374369028	29.836386132019438	25.961325992825614	20.577849500785923
38	22.980459267547715	30.69694469388171	23.224346226236204	23.098249812334373
39	22.917655927021187	29.013752481966076	24.867380344552526	23.20121124646021
40	24.37006643128147	24.97488649951497	25.013399090742404	25.641647978461158
41	19.854768744230945	24.53847576331721	25.99883952904155	29.607915963410296
42	22.961790651352523	26.250875642333604	23.761955358304224	27.02537834800965
43	22.9934665720111	25.04711230005187	25.705567909882888	26.25385321805414
44	21.83142846844475	27.83845241285498	26.384964496327395	23.945154622372876
45	20.91184730364847	30.7000593947999	23.17449142223121	25.213601879320425
46	23.75272879143336	28.681635253242817	25.368122387764984	22.197513567558843
47	22.78877783053833	26.254695558554026	26.614277005436428	24.342249605471217
48	24.104454923421454	23.72397167855346	28.240013759534122	23.931559638490967
49	22.460167089012277	22.948234846098515	29.606662247317438	24.98493581757177
50	20.972476230326453	28.50415606884782	26.734300732013484	23.789066968812243
51	20.29691914941632	28.78590917140336	25.491809316050855	25.425362363129462
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1036.0
1	2699.0
2	4362.0
3	13124.0
4	21886.0
5	16436.5
6	10987.0
7	11354.0
8	11721.0
9	12444.0
10	13167.0
11	13250.5
12	13334.0
13	12868.5
14	12403.0
15	11821.5
16	11240.0
17	10774.5
18	10309.0
19	10804.0
20	11299.0
21	11438.5
22	11578.0
23	12088.5
24	12599.0
25	16037.5
26	21265.0
27	23054.0
28	27925.0
29	32796.0
30	40625.5
31	48455.0
32	57408.0
33	66361.0
34	81152.5
35	95944.0
36	105783.5
37	115623.0
38	139863.0
39	164103.0
40	220313.0
41	276523.0
42	336964.0
43	397405.0
44	402660.5
45	407916.0
46	414360.5
47	420805.0
48	422734.5
49	424664.0
50	418583.5
51	412503.0
52	390523.0
53	368543.0
54	349383.5
55	330224.0
56	311924.5
57	293625.0
58	277171.0
59	260717.0
60	248728.5
61	236740.0
62	210702.0
63	184664.0
64	161006.0
65	137348.0
66	111845.5
67	86343.0
68	70307.0
69	54271.0
70	46657.5
71	39044.0
72	32816.5
73	26589.0
74	21108.0
75	12864.5
76	10102.0
77	7956.5
78	5811.0
79	3978.5
80	2146.0
81	1510.0
82	874.0
83	574.0
84	274.0
85	216.0
86	158.0
87	111.5
88	65.0
89	56.0
90	47.0
91	41.5
92	36.0
93	30.5
94	25.0
95	13.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5104824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.375016618085645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95094181874552	12.92822984295471
2	9.029430664351924	2.957141543612298
3	3.015488262582216	1.4813601123427804
4	1.403355461874815	0.9191987603712544
5	0.8027911244518335	0.6572859001875219
6	0.49538280714441785	0.4867141019582256
7	0.3716146408446105	0.4259637143548098
8	0.2710238062512651	0.3550415465009033
9	0.21452479092774038	0.31615623147897876
>10	2.3535319733794986	9.165364295239533
>50	1.16980912087427	14.175334182432367
>100	1.9078448449030334	53.6189325776226
>500	0.01136020563455041	1.2491877583600117
>1k	0.002537918280059127	0.7298060319186416
>5k	1.2085325143138698E-4	0.10739891900923228
>10k+	2.4170650286277397E-4	0.4268844816561912
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11386	0.2230439286447486	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10185	0.19951716258973864	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5427	0.10631120681143953	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.041901542540937746	0.0
2	0.0	0.0	0.0	0.1696238695006919	0.0
3	0.0	0.0	0.0	0.2565612448147086	0.0
4	0.0	0.0	0.0	0.4213661430834834	0.0
5	0.0	0.0	0.0	0.7587724865734842	0.0
6	0.0	0.0	0.0	1.1360822625814329	0.0
7	1.958931395088254E-5	0.0	0.0	1.3432980255538682	0.0
8	1.958931395088254E-5	0.0	0.0	1.8565576403809416	0.0
9	1.958931395088254E-5	0.0	0.0	2.0630485987371943	0.0
10	1.958931395088254E-5	0.0	0.0	2.404372805017372	0.0
11	1.958931395088254E-5	0.0	0.0	2.7478322465181955	0.0
12	1.958931395088254E-5	0.0	0.0	3.0331310148988484	0.0
13	1.958931395088254E-5	0.0	0.0	3.153644474324678	0.0
14	1.958931395088254E-5	0.0	0.0	3.2009526675160593	0.0
15	1.958931395088254E-5	0.0	0.0	3.2789377263545227	0.0
16	1.958931395088254E-5	0.0	0.0	3.4521072616803243	0.0
17	1.958931395088254E-5	0.0	0.0	3.6480787584449534	0.0
18	1.958931395088254E-5	0.0	0.0	3.899292120551071	0.0
19	1.958931395088254E-5	0.0	0.0	4.030305452254574	0.0
20	1.958931395088254E-5	0.0	0.0	4.167959561387425	0.0
21	1.958931395088254E-5	0.0	0.0	4.357780013571476	0.0
22	1.958931395088254E-5	0.0	0.0	4.553908224847713	0.0
23	1.958931395088254E-5	0.0	0.0	4.783299091212547	0.0
24	1.958931395088254E-5	0.0	0.0	4.95893688009616	0.0
25	1.958931395088254E-5	0.0	0.0	5.107404290529899	0.0
26	1.958931395088254E-5	0.0	0.0	5.255068539091651	0.0
27	1.958931395088254E-5	0.0	0.0	5.39687558278209	0.0
28	1.958931395088254E-5	0.0	0.0	5.55157239505221	0.0
29	1.958931395088254E-5	0.0	0.0	5.719041440018304	0.0
30	1.958931395088254E-5	0.0	0.0	5.920830963026345	0.0
31	1.958931395088254E-5	0.0	0.0	6.096429573282056	0.0
32	1.958931395088254E-5	0.0	0.0	6.266445229061766	0.0
33	1.958931395088254E-5	0.0	0.0	6.447254596828412	0.0
34	1.958931395088254E-5	0.0	0.0	6.641698127104871	0.0
35	1.958931395088254E-5	0.0	0.0	6.861039675412903	0.0
36	1.958931395088254E-5	0.0	0.0	7.045061690667494	0.0
37	1.958931395088254E-5	0.0	0.0	7.239975364478775	0.0
38	1.958931395088254E-5	0.0	0.0	7.456202211868617	0.0
39	1.958931395088254E-5	0.0	0.0	7.715486371322498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9690	0.0	40.23994	1
TAAACGG	255	0.0	39.705883	2
AAGCGTA	40	3.4613367E-7	39.375	17
TGCGATT	40	3.4613367E-7	39.375	16
TTGCGTC	440	0.0	39.375	16
GCGTACC	40	3.4613367E-7	39.375	9
CGTAAGG	710	0.0	38.978874	2
TCGTTGA	965	0.0	38.704662	24
CGTAAAT	70	0.0	38.57143	16
CGTTGAT	960	0.0	38.437504	25
ACTACGG	190	0.0	37.894737	2
AGGGCGA	5275	0.0	37.535545	6
GTACGAT	30	1.1404723E-4	37.500004	11
TACGTAG	270	0.0	37.5	1
GCGATAT	565	0.0	37.0354	9
ATTATAC	3435	0.0	36.94323	42
GGGCGAT	10515	0.0	36.847363	7
ATGATCG	1380	0.0	36.684784	27
ATTAGCG	485	0.0	36.649487	1
TATACTA	3405	0.0	36.60793	44
>>END_MODULE