FastQCFastQC Report
Thu 26 May 2016
SRR1545359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545359_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1878334
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42800.2278614985407281No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC40590.2160957529385083No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG207.0344296E-445.00000410
GTATCGA207.0344296E-445.0000049
CGGTAAT207.0344296E-445.00000435
ACGTTAG451.929402E-840.01
CGCATCG451.929402E-840.021
TATACGT451.929402E-840.023
TATGGGT3750.039.6000024
TCGTTAC403.4597906E-739.37500437
GTTACGG1950.039.230772
TACGTAG1100.038.8636361
ATAGACG356.250406E-638.5714261
TCGAGAC356.250406E-638.57142624
ATACCGG700.038.5714262
TGCGTAT356.250406E-638.57142617
AGTAACG659.094947E-1238.0769271
GTTGATC5200.037.64423416
CGTTTTT39350.037.6238861
TGACGTA301.1401976E-437.50000413
GCGATCG301.1401976E-437.5000049
ACGTATA301.1401976E-437.50000429