##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545359_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1878334
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.161062941947492	31.0	31.0	33.0	30.0	34.0
2	31.517595379735447	31.0	31.0	34.0	30.0	34.0
3	31.702400638012197	31.0	31.0	34.0	30.0	34.0
4	35.49010080209377	37.0	35.0	37.0	33.0	37.0
5	35.396841562789156	37.0	35.0	37.0	33.0	37.0
6	35.45345396505627	37.0	35.0	37.0	33.0	37.0
7	35.80309359251336	37.0	35.0	37.0	35.0	37.0
8	35.83810653483353	37.0	35.0	37.0	35.0	37.0
9	37.70991953507736	39.0	37.0	39.0	35.0	39.0
10	37.073617897562414	39.0	37.0	39.0	33.0	39.0
11	36.69618608831017	39.0	35.0	39.0	32.0	39.0
12	35.87202116343526	37.0	35.0	39.0	31.0	39.0
13	35.49065235469304	37.0	35.0	39.0	30.0	39.0
14	36.43492744101954	38.0	35.0	41.0	31.0	41.0
15	36.72513088726499	38.0	35.0	41.0	31.0	41.0
16	36.82718302495722	38.0	35.0	41.0	32.0	41.0
17	36.75491313046562	38.0	35.0	41.0	32.0	41.0
18	36.70749664330199	38.0	35.0	40.0	31.0	41.0
19	36.66591032265827	38.0	35.0	40.0	31.0	41.0
20	36.536500963087505	38.0	35.0	40.0	31.0	41.0
21	36.39277146662947	38.0	35.0	40.0	31.0	41.0
22	36.259736021389166	38.0	35.0	40.0	30.0	41.0
23	36.181725933726376	38.0	35.0	40.0	30.0	41.0
24	36.07649544756151	37.0	35.0	40.0	30.0	41.0
25	35.94638706428143	37.0	34.0	40.0	30.0	41.0
26	35.76308846030578	37.0	34.0	40.0	30.0	41.0
27	35.615186117059054	37.0	34.0	40.0	29.0	41.0
28	35.535720484216334	37.0	34.0	40.0	29.0	41.0
29	35.67920827712217	37.0	34.0	40.0	30.0	41.0
30	35.65560384894273	37.0	34.0	40.0	29.0	41.0
31	35.53851924098696	37.0	34.0	40.0	29.0	41.0
32	35.392825770070715	37.0	34.0	40.0	29.0	41.0
33	35.23974969307908	37.0	34.0	40.0	28.0	41.0
34	35.08498541792887	36.0	34.0	40.0	27.0	41.0
35	34.96824100506087	36.0	34.0	40.0	27.0	41.0
36	34.79958782623325	36.0	33.0	40.0	26.0	41.0
37	34.69938679702332	36.0	33.0	40.0	26.0	41.0
38	34.620776177186805	36.0	33.0	40.0	26.0	41.0
39	34.55645481581018	36.0	33.0	40.0	25.0	41.0
40	34.400176965332044	36.0	33.0	40.0	24.0	41.0
41	34.325429875623826	35.0	33.0	40.0	24.0	41.0
42	34.262141876790814	35.0	33.0	40.0	24.0	41.0
43	34.13336552498118	35.0	33.0	40.0	24.0	41.0
44	34.02107612384166	35.0	33.0	40.0	23.0	41.0
45	33.95877250797781	35.0	33.0	40.0	23.0	41.0
46	33.98352955331693	35.0	33.0	39.0	24.0	41.0
47	33.93400215297173	35.0	33.0	39.0	23.0	41.0
48	33.85618053019324	35.0	33.0	39.0	23.0	41.0
49	33.81624141393384	35.0	33.0	39.0	24.0	41.0
50	33.68490268503898	35.0	33.0	39.0	24.0	41.0
51	33.429152642714236	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	5.0
12	4.0
13	8.0
14	15.0
15	99.0
16	240.0
17	524.0
18	1099.0
19	2072.0
20	3629.0
21	5692.0
22	8408.0
23	12037.0
24	16536.0
25	22287.0
26	29185.0
27	35399.0
28	40305.0
29	46844.0
30	55879.0
31	67411.0
32	83408.0
33	107619.0
34	185301.0
35	259145.0
36	142041.0
37	174434.0
38	236342.0
39	342156.0
40	204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.05255188906765	23.58270680294346	28.20680454061951	13.157936767369382
2	31.427690708894158	25.738287226872323	29.547034765914905	13.286987298318618
3	30.19675946876328	25.731632393386906	29.885206784309926	14.186401353539893
4	27.07963546419327	27.734311363154795	29.283822791899627	15.902230380752306
5	24.327089857288428	32.33567618964465	28.20951971268156	15.127714240385362
6	23.168190534803713	41.153490273827764	24.969094953293716	10.709224238074805
7	88.42468911279889	4.235881371470676	5.410326384977326	1.9291031307531035
8	89.3361351069618	3.0631399953362926	5.27983840999524	2.3208864877066593
9	84.69212610749739	4.637620359318417	7.302748073558803	3.3675054596253915
10	53.13554458365765	20.40797855972367	12.802994568591103	13.653482288027583
11	44.040090846462874	21.72936229658836	18.256923422564892	15.973623434383875
12	37.785079756848354	21.158164628867922	24.27752465748903	16.77923095679469
13	23.003896005715703	34.32989021121909	25.36785257573999	17.298361207325215
14	18.515876303149494	36.29008472401607	27.52167612362871	17.672362849205733
15	18.133569429079174	21.84877662865071	42.552336272462725	17.465317669807394
16	20.28505047558102	18.10428816174333	40.58660493820588	21.02405642446977
17	20.40941600375652	18.514758291123943	27.320859868372715	33.75496583674682
18	24.839299080994117	22.097294730330177	30.960042250206833	22.103363938468878
19	30.87810794033436	23.23127835624548	23.608101647523817	22.28251205589634
20	32.50806299625093	22.253124311224735	22.53422447764881	22.704588214875525
21	26.12996410649011	26.278659705888302	25.01993788112231	22.57143830649927
22	25.978233903022574	23.804339377341837	23.847462698327348	26.369964021308245
23	23.647338545753843	28.57228799563869	23.400843513453946	24.379529945153525
24	23.76611401380159	23.456850592067223	32.75109751513842	20.025937878992767
25	21.503310912755666	23.13252062732187	31.323023487835496	24.04114497208697
26	20.998714818557296	29.883822578944958	25.27239564422515	23.845066958272596
27	22.21953071179034	28.530815073357562	26.066929523716233	23.182724691135867
28	19.435308097494907	26.711223882440503	33.14900331889856	20.704464701166035
29	20.472929734541353	24.363771299460055	32.22217134971736	22.941127616281236
30	22.22698412529401	26.72948474552449	28.72465706312083	22.31887406606067
31	29.895801279218716	23.864232878710602	23.202742430260006	23.03722341181068
32	30.069678768525726	24.460293004332563	24.523167871102796	20.946860356038915
33	27.91154288853846	24.476584036704867	24.83631771559265	22.775555359164027
34	21.65780952695314	25.092395708111546	28.428277399014235	24.82151736592108
35	22.69644269868937	24.117169789824384	29.12527803894302	24.061109472543222
36	29.75402670664536	24.352804133876084	24.837701920957613	21.055467238520944
37	23.43944154766937	28.495091927207834	26.460310040706286	21.60515648441651
38	23.53819927659298	30.087992870277596	22.59608780972926	23.777720043400162
39	23.273762813216393	28.839705824416743	24.45544828555518	23.431083076811685
40	25.08771070533782	24.434099579733957	24.06238720057242	26.415802514355807
41	20.807854194195492	23.17127837754095	25.442706142783976	30.578161285479577
42	23.4208080139102	25.305456856980708	23.432893191519717	27.84084193758937
43	23.166912806774516	25.29635304477266	25.563770873550713	25.97296327490212
44	22.528687656188943	27.811720386257182	26.523664055487465	23.135927902066406
45	20.625777950034447	30.439048646300392	23.364428264621733	25.570745139043428
46	23.321145227632574	28.921373940949803	25.493708786616224	22.263772044801403
47	23.62135807582677	24.394330294825096	27.272199725927337	24.712111903420798
48	25.15755983760077	21.625014507537	28.79205721666115	24.425368438201087
49	22.33207725569574	22.500364684875002	30.218747038599098	24.94881102083016
50	21.25324888970758	28.857008391478832	26.162439693898953	23.72730302491463
51	20.790977536476476	29.97118723294153	24.245794411430555	24.99204081915144
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	508.0
1	1326.5
2	2145.0
3	5549.5
4	8954.0
5	6787.0
6	4620.0
7	4689.5
8	4759.0
9	4937.0
10	5115.0
11	5153.0
12	5191.0
13	5301.5
14	5412.0
15	5157.0
16	4902.0
17	4649.0
18	4396.0
19	4272.5
20	4149.0
21	4643.0
22	5137.0
23	5263.5
24	5390.0
25	6114.5
26	8199.5
27	9560.0
28	11984.0
29	14408.0
30	16346.0
31	18284.0
32	20871.0
33	23458.0
34	27040.5
35	30623.0
36	33227.0
37	35831.0
38	44292.5
39	52754.0
40	75297.0
41	97840.0
42	122199.5
43	146559.0
44	155144.0
45	163729.0
46	160738.5
47	157748.0
48	149451.0
49	141154.0
50	135414.0
51	129674.0
52	123714.0
53	117754.0
54	112490.5
55	107227.0
56	107376.0
57	107525.0
58	103165.5
59	98806.0
60	96566.5
61	94327.0
62	84967.0
63	75607.0
64	68709.5
65	61812.0
66	51936.5
67	42061.0
68	35338.5
69	28616.0
70	24078.5
71	19541.0
72	17220.0
73	14899.0
74	11859.5
75	7453.0
76	6086.0
77	4539.0
78	2992.0
79	2489.5
80	1987.0
81	1256.5
82	526.0
83	437.5
84	349.0
85	249.0
86	149.0
87	96.5
88	44.0
89	36.5
90	29.0
91	28.5
92	28.0
93	17.5
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1878334.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.955994902728918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.0343622873905	15.97165325850324
2	6.8053095873592175	2.716134468736655
3	2.103426153498474	1.2592788479244672
4	1.0238576530346717	0.817283924203196
5	0.6370044036796113	0.6356028316423098
6	0.45455764828545775	0.5442690067308623
7	0.36954103411052097	0.5162191295141111
8	0.318862456067706	0.5090574038367012
9	0.25224229852688695	0.4530371421289664
>10	4.796951967012988	25.608254949371716
>50	2.778973237817793	39.09530302431692
>100	0.42115811370868905	10.981442463200128
>500	0.0029489110416300213	0.3736876810925978
>1k	8.042484658990968E-4	0.5187758687981275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4280	0.2278614985407281	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4059	0.2160957529385083	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04679678906946262	0.0
2	0.0	0.0	0.0	0.16759532649677852	0.0
3	0.0	0.0	0.0	0.2487310563510004	0.0
4	0.0	0.0	0.0	0.4169652468623791	0.0
5	0.0	0.0	0.0	0.7269740099471127	0.0
6	0.0	0.0	0.0	1.099112298451713	0.0
7	0.0	0.0	0.0	1.3106295259522533	0.0
8	0.0	0.0	0.0	1.8355095526141783	0.0
9	0.0	0.0	0.0	2.055757921647588	0.0
10	0.0	0.0	0.0	2.3809397050790753	0.0
11	0.0	0.0	0.0	2.708091319222247	0.0
12	0.0	0.0	0.0	2.9806732987849873	0.0
13	0.0	0.0	0.0	3.099661721504269	0.0
14	0.0	0.0	0.0	3.147044135920449	0.0
15	0.0	0.0	0.0	3.216254404168801	0.0
16	0.0	0.0	0.0	3.3746926797896433	0.0
17	0.0	0.0	0.0	3.546280906377673	0.0
18	0.0	0.0	0.0	3.7779755890060023	0.0
19	0.0	0.0	0.0	3.898028785082951	0.0
20	0.0	0.0	0.0	4.021382778568667	0.0
21	0.0	0.0	0.0	4.1914270837880805	0.0
22	0.0	0.0	0.0	4.358490023606025	0.0
23	0.0	0.0	0.0	4.554035650741562	0.0
24	0.0	0.0	0.0	4.706564434227353	0.0
25	0.0	0.0	0.0	4.829332802366352	0.0
26	0.0	0.0	0.0	4.961045266709754	0.0
27	0.0	0.0	0.0	5.07833005205677	0.0
28	0.0	0.0	0.0	5.20461217227607	0.0
29	0.0	0.0	0.0	5.340796684721673	0.0
30	0.0	0.0	0.0	5.514461219357154	0.0
31	0.0	0.0	0.0	5.6721009149597466	0.0
32	0.0	0.0	0.0	5.821062707697353	0.0
33	0.0	0.0	0.0	5.9725267178254775	0.0
34	0.0	0.0	0.0	6.135277325544871	0.0
35	0.0	0.0	0.0	6.3165017510197865	0.0
36	0.0	0.0	0.0	6.4758983226625295	0.0
37	0.0	0.0	0.0	6.640033135746891	0.0
38	0.0	0.0	0.0	6.815241591750988	0.0
39	0.0	0.0	0.0	6.989598229068951	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACCG	20	7.0344296E-4	45.000004	10
GTATCGA	20	7.0344296E-4	45.000004	9
CGGTAAT	20	7.0344296E-4	45.000004	35
ACGTTAG	45	1.929402E-8	40.0	1
CGCATCG	45	1.929402E-8	40.0	21
TATACGT	45	1.929402E-8	40.0	23
TATGGGT	375	0.0	39.600002	4
TCGTTAC	40	3.4597906E-7	39.375004	37
GTTACGG	195	0.0	39.23077	2
TACGTAG	110	0.0	38.863636	1
ATAGACG	35	6.250406E-6	38.571426	1
TCGAGAC	35	6.250406E-6	38.571426	24
ATACCGG	70	0.0	38.571426	2
TGCGTAT	35	6.250406E-6	38.571426	17
AGTAACG	65	9.094947E-12	38.076927	1
GTTGATC	520	0.0	37.644234	16
CGTTTTT	3935	0.0	37.623886	1
TGACGTA	30	1.1401976E-4	37.500004	13
GCGATCG	30	1.1401976E-4	37.500004	9
ACGTATA	30	1.1401976E-4	37.500004	29
>>END_MODULE