FastQCFastQC Report
Thu 26 May 2016
SRR1545358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545358_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3416425
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC91760.268584851123616No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77580.2270794763532055No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT48090.14076117579048275No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA207.035242E-445.00000438
CGTGCTA551.8189894E-1245.013
GCGTAAG3500.041.142861
TATAGCG2800.040.982141
CGTTTTT71450.040.591321
TACGTAG1450.038.7931061
ACACCGT356.2519102E-638.57142643
CCGTAAC356.2519102E-638.57142634
ACCGTAT356.2519102E-638.57142620
TCGACGA356.2519102E-638.57142628
AATACGG2950.038.135592
GTAGCGT659.094947E-1238.0769279
TGTAACG1550.037.7419361
TACGGGA7950.037.6415064
CGTTAGG4850.037.577322
AGGGTAT17400.037.56
ACCTACG301.14039336E-437.49999635
ACGTCGA301.14039336E-437.49999634
GCGTAGC601.5643309E-1037.4999969
GCGTAAT601.5643309E-1037.4999969