FastQCFastQC Report
Thu 26 May 2016
SRR1545356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545356_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3306377
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178910.5411058690524402No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT155050.042.3298951
AGGGCGA39400.039.8604056
GGGCGAT81900.038.4615367
TAGACGG4450.038.4269642
TCGTAAG2250.038.01
TATTACG850.037.0588231
ACACGAC8150.036.99386626
CACGACC8400.036.4285727
TACGGGA8400.036.428574
AAGGGCG18100.036.1740345
GGCGATA18050.035.7756238
GTTTTTT197050.035.499872
CGTAAGG5800.035.3017272
AACACGT8300.035.24096341
ACGGGTA2950.035.0847435
TGGGCGA25600.035.068366
TATGGGC12650.035.0395284
TCGATAG1800.035.01
GGCGATT24500.034.98988
GACCGAT19200.034.804699
Produced by FastQC (version 0.11.5)