FastQCFastQC Report
Thu 26 May 2016
SRR1545355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545355_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3514449
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81400.23161525462455138No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC75820.21573794355815093No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT351.212884E-745.035
ACGTCGT207.03527E-445.039
TTACGTA253.8924503E-545.020
CGTTTCA207.03527E-445.023
AATCGGC207.03527E-445.010
CATACGT302.166782E-644.99999642
GTACGTC302.166782E-644.9999963
ATATACG1000.042.751
TTTTACG900.042.51
CTAAGCG1400.041.7857131
AATGCGG4200.041.7857132
ATGCGAA556.184564E-1140.90909219
AAATGCG1050.040.7142871
CGGTTAT501.0822987E-940.538
CGTAAGG4700.039.7340432
TCGCTAA403.4609366E-739.37540
CATCGCA403.4609366E-739.37519
ATAGGGC14350.037.944254
AGGGCGA39900.037.8947376
GGGCGAT76850.037.621997
Produced by FastQC (version 0.11.5)