##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545355_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3514449
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17877795352842	31.0	31.0	33.0	30.0	34.0
2	31.53493477924989	31.0	31.0	34.0	30.0	34.0
3	31.720502132766757	31.0	31.0	34.0	30.0	34.0
4	35.507227164201275	37.0	35.0	37.0	33.0	37.0
5	35.405230236660145	37.0	35.0	37.0	33.0	37.0
6	35.458260171082294	37.0	35.0	37.0	33.0	37.0
7	35.8251068659696	37.0	35.0	37.0	35.0	37.0
8	35.86443650199505	37.0	35.0	37.0	35.0	37.0
9	37.74273150641822	39.0	37.0	39.0	35.0	39.0
10	37.09983129645643	39.0	37.0	39.0	33.0	39.0
11	36.69768347755224	39.0	35.0	39.0	32.0	39.0
12	35.80704628236176	37.0	35.0	39.0	31.0	39.0
13	35.40579106426071	37.0	35.0	39.0	30.0	39.0
14	36.33501894607092	38.0	35.0	41.0	31.0	41.0
15	36.653720682815425	38.0	35.0	41.0	31.0	41.0
16	36.79275727148125	38.0	35.0	41.0	32.0	41.0
17	36.74871309841173	38.0	35.0	41.0	32.0	41.0
18	36.6863946524761	38.0	35.0	40.0	31.0	41.0
19	36.62991154516683	38.0	35.0	40.0	31.0	41.0
20	36.492894618758164	38.0	35.0	40.0	31.0	41.0
21	36.36364078693417	38.0	35.0	40.0	31.0	41.0
22	36.22421836253706	38.0	35.0	40.0	30.0	41.0
23	36.151182731631614	37.0	35.0	40.0	30.0	41.0
24	36.03640371506316	37.0	35.0	40.0	30.0	41.0
25	35.91389290326876	37.0	34.0	40.0	30.0	41.0
26	35.72894470797556	37.0	34.0	40.0	30.0	41.0
27	35.6121946854258	37.0	34.0	40.0	29.0	41.0
28	35.532608098737526	36.0	34.0	40.0	29.0	41.0
29	35.69795663559209	37.0	34.0	40.0	30.0	41.0
30	35.7067611452037	37.0	34.0	40.0	30.0	41.0
31	35.576728243886876	37.0	34.0	40.0	29.0	41.0
32	35.43836516051307	37.0	34.0	40.0	29.0	41.0
33	35.27442737111849	36.0	34.0	40.0	29.0	41.0
34	35.1397200528447	36.0	34.0	40.0	27.0	41.0
35	35.02083655218784	36.0	34.0	40.0	27.0	41.0
36	34.83940640481623	36.0	34.0	40.0	26.0	41.0
37	34.704209678387706	36.0	33.0	40.0	26.0	41.0
38	34.64614623800203	36.0	33.0	40.0	26.0	41.0
39	34.58617780482801	36.0	33.0	40.0	26.0	41.0
40	34.42842021608508	35.0	33.0	40.0	25.0	41.0
41	34.37526707600537	35.0	33.0	40.0	24.0	41.0
42	34.30375202485511	35.0	33.0	40.0	24.0	41.0
43	34.1747133049875	35.0	33.0	40.0	24.0	41.0
44	34.04349586521244	35.0	33.0	40.0	24.0	41.0
45	34.00536357192834	35.0	33.0	40.0	24.0	41.0
46	34.01171705721153	35.0	33.0	39.0	24.0	41.0
47	33.98033034481365	35.0	33.0	39.0	24.0	41.0
48	33.894560712077485	35.0	33.0	39.0	24.0	41.0
49	33.862322088042816	35.0	33.0	39.0	24.0	41.0
50	33.71877924533832	35.0	33.0	39.0	24.0	41.0
51	33.46813881777769	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	7.0
11	4.0
12	11.0
13	18.0
14	50.0
15	115.0
16	340.0
17	858.0
18	1872.0
19	3622.0
20	6392.0
21	10016.0
22	14922.0
23	21617.0
24	30492.0
25	41186.0
26	54019.0
27	65612.0
28	74838.0
29	86934.0
30	103707.0
31	126035.0
32	155532.0
33	201939.0
34	352971.0
35	510679.0
36	258734.0
37	318267.0
38	428393.0
39	644876.0
40	389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.161642692780575	23.49324175710047	28.488078785607645	12.857036764511307
2	30.453564698193087	25.42005873466936	30.142534434274047	13.983842132863503
3	29.714103121143598	25.53595172386909	30.284804246696993	14.46514090829032
4	26.614698349584813	28.194604616541596	29.717830590229077	15.47286644364451
5	23.870911201158417	32.86082683231425	28.707658014101217	14.560603952426113
6	22.79828217737688	41.758779256719905	24.96018579299344	10.482752772909778
7	88.83540492407202	4.05321004800468	5.298440808217732	1.812944219705564
8	90.0118909109223	2.9220796773548283	5.026790828377364	2.0392385833454973
9	85.16472994771014	4.643146052197656	7.005337109743234	3.186786890348957
10	54.394472647063594	20.345977420642612	12.508646447850005	12.750903484443793
11	45.77144240818404	20.807415330255182	17.96034029800973	15.460801963551043
12	39.36326860910487	21.395416464999208	23.29992553598018	15.941389389915745
13	22.855958359333144	36.16882760284756	24.23981682477111	16.735397213048188
14	18.09745994322296	38.838862080513906	26.74353789171503	16.320140084548104
15	17.15606059442035	22.211874464531995	43.679620902167024	16.952444038880632
16	18.488019032286427	18.028772077785167	41.97141571836723	21.511793171561173
17	19.554274368471418	18.487421499074248	27.4870968393623	34.471207293092036
18	24.299342514288867	21.832953046124725	31.405435105190033	22.462269334396375
19	30.813564231548103	22.925129942133175	23.58944460426087	22.671861222057853
20	31.898627636935405	22.996606295894466	22.918073359437	22.186692707733133
21	25.416957252758543	26.149020799562035	25.267858489339297	23.16616345834013
22	25.61044419765374	23.379255183387208	23.837449341276542	27.172851277682504
23	23.498704917897513	28.46366528579587	23.080943840698783	24.956685955607835
24	23.010434921661975	23.627430644177792	33.75928915172762	19.60284528243261
25	21.08512600410477	24.184360051888646	31.811757689469957	22.91875625453663
26	20.381971683185615	30.65752270128262	25.451699540952223	23.508806074579542
27	22.295415298386743	29.286440065000228	26.46497359899091	21.953171037622113
28	18.69527769502417	27.12672740449499	34.001660004171356	20.17633489630949
29	19.079776090078415	24.172665473307482	33.305562265948375	23.44199617066573
30	21.49682638729428	27.045747427263844	29.134211365707685	22.32321481973419
31	29.522721769472255	24.84434971171868	23.01791831379542	22.615010205013643
32	29.652443384439497	26.252137959606188	23.886959235999726	20.208459419954593
33	27.835714787723482	25.63642266540217	24.52227931035562	22.005583236518724
34	21.30917250470842	26.35798670004886	27.634403003145014	24.698437792097707
35	22.992651195109104	25.06907910742196	28.443519880356778	23.494749817112158
36	30.10525974341924	24.45649374909125	24.531128492688328	20.90711801480118
37	23.585290325738118	29.357518063286737	26.231565744729828	20.82562586624532
38	23.398148614476977	31.15609303193758	22.760495315197346	22.685263038388097
39	22.95423834575491	29.23917803331333	24.906891521259805	22.899692099671952
40	24.41500787178872	25.53378922272026	24.288273922882365	25.762928982608653
41	20.361314106421805	23.556580277591166	25.350460342432058	30.731645273554975
42	23.358626060585884	26.182539567368885	23.27394706823175	27.184887303813486
43	23.738088104280358	25.915470675488532	24.95019845216135	25.39624276806976
44	22.4330471149247	29.093180751804905	25.77220497437863	22.701567158891763
45	20.31601539814634	31.93282929984188	22.458997128710646	25.292158173301132
46	23.655429343262625	29.34075867938331	25.476113040763998	21.52769893659006
47	24.50876367817544	24.9177893888914	26.249064931657852	24.324382001275307
48	24.69385670413769	22.616660534837752	28.206526826822643	24.482955934201918
49	22.53747315724314	22.560577774780626	29.76233258755498	25.139616480421257
50	20.815353985788384	29.642968214932125	26.267104743873084	23.274573055406407
51	20.90660584347646	30.88509749323436	23.46931197465093	24.738984688638247
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	761.0
1	2584.5
2	4408.0
3	10495.5
4	16583.0
5	12522.0
6	8461.0
7	8638.5
8	8816.0
9	9165.0
10	9514.0
11	9826.0
12	10138.0
13	10164.0
14	10190.0
15	10010.5
16	9831.0
17	9227.5
18	8624.0
19	8693.5
20	8763.0
21	8383.0
22	8003.0
23	9130.0
24	10257.0
25	11309.5
26	16253.5
27	20145.0
28	22652.0
29	25159.0
30	30978.5
31	36798.0
32	44585.5
33	52373.0
34	54852.0
35	57331.0
36	61119.5
37	64908.0
38	83555.0
39	102202.0
40	146272.0
41	190342.0
42	244919.0
43	299496.0
44	310729.5
45	321963.0
46	320137.5
47	318312.0
48	292142.5
49	265973.0
50	256940.5
51	247908.0
52	233695.0
53	219482.0
54	207851.5
55	196221.0
56	187931.5
57	179642.0
58	175222.0
59	170802.0
60	162922.0
61	155042.0
62	145284.0
63	135526.0
64	118120.5
65	100715.0
66	88280.5
67	75846.0
68	63973.5
69	52101.0
70	43953.0
71	35805.0
72	31911.5
73	28018.0
74	22456.5
75	13586.0
76	10277.0
77	7475.0
78	4673.0
79	3345.0
80	2017.0
81	1608.0
82	1199.0
83	723.0
84	247.0
85	195.0
86	143.0
87	122.5
88	102.0
89	69.5
90	37.0
91	34.0
92	31.0
93	18.0
94	5.0
95	2.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3514449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.44575122051765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00052938194482	13.15668803725177
2	8.461588941184917	2.78314373314021
3	2.754411624272455	1.358951049950602
4	1.2513178697372402	0.8231544953394706
5	0.7074748662329868	0.5817477822418353
6	0.4534127435470689	0.44740279083524787
7	0.35294921896299924	0.4063160533979047
8	0.2578473276654549	0.33923944029290953
9	0.19533263825497757	0.2891152776589857
>10	2.2607082994282837	9.102514077928316
>50	1.2523162415713074	15.255337591118131
>100	2.039336576213308	53.31990837934379
>500	0.010149420781976563	1.0961125477266853
>1k	0.002274870175270609	0.5879143887184534
>5k	3.4998002696470906E-4	0.452454355055633
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8140	0.23161525462455138	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7582	0.21573794355815093	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0512740403972287	0.0
2	0.0	0.0	0.0	0.16870354357112594	0.0
3	0.0	0.0	0.0	0.25025260005195693	0.0
4	0.0	0.0	0.0	0.4401258917116168	0.0
5	0.0	0.0	0.0	0.7507008922309016	0.0
6	0.0	0.0	0.0	1.145414259817115	0.0
7	0.0	0.0	0.0	1.358164537314384	0.0
8	0.0	0.0	0.0	1.947616824145122	0.0
9	0.0	0.0	0.0	2.186459385240759	0.0
10	0.0	0.0	0.0	2.540312862699103	0.0
11	0.0	0.0	0.0	2.882841663088581	0.0
12	0.0	0.0	0.0	3.1800433012401093	0.0
13	0.0	0.0	0.0	3.3052122822098142	0.0
14	0.0	0.0	0.0	3.349856549348134	0.0
15	0.0	0.0	0.0	3.4195403034728913	0.0
16	0.0	0.0	0.0	3.5743867673140226	0.0
17	0.0	0.0	0.0	3.751228144155741	0.0
18	0.0	0.0	0.0	3.9872253090029193	0.0
19	0.0	0.0	0.0	4.1051669835015385	0.0
20	0.0	0.0	0.0	4.23235619580765	0.0
21	0.0	0.0	0.0	4.393121083845576	0.0
22	0.0	0.0	0.0	4.562166074966517	0.0
23	0.0	0.0	0.0	4.7535474266378595	0.0
24	0.0	0.0	0.0	4.911097016915027	0.0
25	0.0	0.0	0.0	5.03327833182385	0.0
26	2.845396248458862E-5	0.0	0.0	5.1586180365684635	0.0
27	2.845396248458862E-5	0.0	0.0	5.270868918570166	0.0
28	2.845396248458862E-5	0.0	0.0	5.395753359915025	0.0
29	2.845396248458862E-5	0.0	0.0	5.524336816382881	0.0
30	2.845396248458862E-5	0.0	0.0	5.69528822299029	0.0
31	2.845396248458862E-5	0.0	0.0	5.850646858156144	0.0
32	2.845396248458862E-5	0.0	0.0	6.002164208386578	0.0
33	2.845396248458862E-5	0.0	0.0	6.148019220082579	0.0
34	2.845396248458862E-5	0.0	0.0	6.3070768703714295	0.0
35	2.845396248458862E-5	0.0	0.0	6.488157887623352	0.0
36	2.845396248458862E-5	0.0	0.0	6.645792839787973	0.0
37	2.845396248458862E-5	0.0	0.0	6.797224828130953	0.0
38	2.845396248458862E-5	0.0	0.0	6.947091848537282	0.0
39	2.845396248458862E-5	0.0	0.0	7.1064909463759465	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTAT	35	1.212884E-7	45.0	35
ACGTCGT	20	7.03527E-4	45.0	39
TTACGTA	25	3.8924503E-5	45.0	20
CGTTTCA	20	7.03527E-4	45.0	23
AATCGGC	20	7.03527E-4	45.0	10
CATACGT	30	2.166782E-6	44.999996	42
GTACGTC	30	2.166782E-6	44.999996	3
ATATACG	100	0.0	42.75	1
TTTTACG	90	0.0	42.5	1
CTAAGCG	140	0.0	41.785713	1
AATGCGG	420	0.0	41.785713	2
ATGCGAA	55	6.184564E-11	40.909092	19
AAATGCG	105	0.0	40.714287	1
CGGTTAT	50	1.0822987E-9	40.5	38
CGTAAGG	470	0.0	39.734043	2
TCGCTAA	40	3.4609366E-7	39.375	40
CATCGCA	40	3.4609366E-7	39.375	19
ATAGGGC	1435	0.0	37.94425	4
AGGGCGA	3990	0.0	37.894737	6
GGGCGAT	7685	0.0	37.62199	7
>>END_MODULE