FastQCFastQC Report
Thu 26 May 2016
SRR1545354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545354_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4755374
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182240.3832295840453348No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC57480.12087377354546666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT177950.042.3953361
GACCGAT27950.038.6404349
AGGGCGA49350.038.2066886
CGTTAGG6950.037.553962
AGTACGG5850.037.3076932
GGGCGAT104400.036.8534477
TTGCGAG6250.036.7199971
ACGGGAT16450.036.382985
GCGAGAC11500.036.021
TATAGCG3250.036.01
TAAGGGA52500.035.871434
ATAGCGG11150.035.112112
TAGGGAC43250.035.0635875
TGGGCGA35450.035.0352636
ATAGGGC25250.034.9306954
TACGGGA16200.034.722224
ACACGAC12150.034.6296326
AGGGATT66300.034.6153836
GGCGATA20900.034.5574158
AGGGACC41150.034.5018236
Produced by FastQC (version 0.11.5)