##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545354_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4755374
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.159220284251038	31.0	31.0	33.0	30.0	34.0
2	31.515825463990844	31.0	31.0	34.0	30.0	34.0
3	31.64726496801303	31.0	31.0	34.0	30.0	34.0
4	35.46136097812706	37.0	35.0	37.0	33.0	37.0
5	35.37740165126865	37.0	35.0	37.0	33.0	37.0
6	35.43438581276678	37.0	35.0	37.0	33.0	37.0
7	35.860896955738916	37.0	35.0	37.0	35.0	37.0
8	35.9166038254825	37.0	35.0	37.0	35.0	37.0
9	37.78453724144515	39.0	37.0	39.0	35.0	39.0
10	37.06963048542554	39.0	37.0	39.0	33.0	39.0
11	36.73237099752827	39.0	35.0	39.0	32.0	39.0
12	36.22631258866285	38.0	35.0	39.0	32.0	39.0
13	36.01976858181922	38.0	35.0	39.0	31.0	39.0
14	37.00056168032209	39.0	35.0	41.0	31.0	41.0
15	37.200857598161576	39.0	35.0	41.0	32.0	41.0
16	37.249588360452826	39.0	35.0	41.0	32.0	41.0
17	37.17265981603129	39.0	35.0	41.0	32.0	41.0
18	37.09255108010432	39.0	35.0	41.0	32.0	41.0
19	37.053229041501254	39.0	35.0	41.0	32.0	41.0
20	36.92678704135574	38.0	35.0	41.0	31.0	41.0
21	36.81109792836484	38.0	35.0	41.0	31.0	41.0
22	36.71542259347004	38.0	35.0	41.0	31.0	41.0
23	36.61150542523049	38.0	35.0	40.0	31.0	41.0
24	36.526552485672	38.0	35.0	40.0	31.0	41.0
25	36.40927863928263	38.0	35.0	40.0	30.0	41.0
26	36.273680682108285	38.0	35.0	40.0	30.0	41.0
27	36.13926622806114	38.0	35.0	40.0	30.0	41.0
28	36.04650801388072	38.0	35.0	40.0	30.0	41.0
29	36.1592659168343	38.0	35.0	40.0	30.0	41.0
30	36.10569116119994	38.0	35.0	40.0	30.0	41.0
31	35.99272002580659	38.0	35.0	40.0	30.0	41.0
32	35.84829836727879	38.0	34.0	40.0	29.0	41.0
33	35.625402754862186	38.0	34.0	40.0	28.0	41.0
34	35.42488414160484	38.0	34.0	40.0	27.0	41.0
35	35.26985595665031	38.0	34.0	40.0	26.0	41.0
36	35.12966298760097	38.0	34.0	40.0	25.0	41.0
37	35.02945825081266	38.0	34.0	40.0	25.0	41.0
38	34.96003679205884	38.0	34.0	40.0	25.0	41.0
39	34.88316103002624	38.0	34.0	40.0	25.0	41.0
40	34.76573430396852	37.0	33.0	40.0	24.0	41.0
41	34.69106173352506	37.0	33.0	40.0	24.0	41.0
42	34.64023439586455	37.0	33.0	40.0	24.0	41.0
43	34.53449907410017	37.0	33.0	40.0	24.0	41.0
44	34.41778922120531	37.0	33.0	40.0	23.0	41.0
45	34.372284703579574	37.0	33.0	40.0	23.0	41.0
46	34.38777538843422	37.0	33.0	40.0	23.0	41.0
47	34.362684196868635	37.0	33.0	40.0	23.0	41.0
48	34.28248629865916	36.0	33.0	40.0	23.0	41.0
49	34.234461684822264	36.0	33.0	40.0	24.0	41.0
50	34.115589646576694	36.0	33.0	40.0	24.0	41.0
51	33.87921580931384	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	6.0
12	9.0
13	22.0
14	43.0
15	130.0
16	472.0
17	1065.0
18	2403.0
19	4723.0
20	8184.0
21	12959.0
22	19463.0
23	28535.0
24	41384.0
25	59403.0
26	78227.0
27	90492.0
28	99076.0
29	111093.0
30	130801.0
31	157115.0
32	191316.0
33	243590.0
34	382873.0
35	509812.0
36	364591.0
37	466499.0
38	667040.0
39	1083594.0
40	449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.6201371332728	23.303046195735604	27.720532601641846	14.356284069349751
2	32.342566536301874	25.057923940367253	28.99420739567487	13.605302127655994
3	28.777442110757217	25.596514595907706	31.725349047204276	13.900694246130799
4	25.43423503598245	27.88956241927554	31.572280119292408	15.103922425449607
5	23.261177774871125	32.14190934298753	30.263739508185893	14.333173373955447
6	21.27843151768925	40.977933596810686	27.320143483982545	10.423491401517525
7	88.19230622028887	3.4257663014517887	6.683112621636068	1.6988148566232646
8	89.16503728203081	2.531178409942099	6.454339868956679	1.8494444390704075
9	84.1440862485264	4.653787483381959	8.315960006510528	2.886166261581108
10	42.608551924622546	29.125175012522675	15.781808118562282	12.4844649442925
11	34.49600388949429	24.99567857333619	24.461356772359018	16.046960764810507
12	30.60362865255183	23.567273573014447	28.53056352665427	17.29853424777946
13	22.773119422362996	31.164720167120397	29.615273162531487	16.446887247985124
14	19.98059458625126	33.00329269580058	30.021886816893893	16.99422590105426
15	19.3505284757834	24.752353863229263	39.14543840295212	16.751679258035225
16	21.353020813925465	22.65908002188682	37.22950077112757	18.758398393060148
17	21.71408600038609	22.349114917144266	29.243840757845756	26.692958324623888
18	23.71802932850287	24.30601672970412	31.419442508622875	20.556511433170137
19	27.717756794733706	24.951496980048258	27.052278117346816	20.27846810787122
20	28.52782977742655	25.018663095689213	26.070189221710006	20.38331790517423
21	25.260074181336734	26.510133587810337	28.618779511348635	19.61101271950429
22	24.656441323016864	24.47832704641107	27.400557768957817	23.464673861614248
23	21.92630905581769	28.36481420809383	27.602203317762182	22.1066734183263
24	21.255404937655797	25.952343601155242	33.24762679023774	19.544624670951222
25	21.19707093490438	26.534548071297863	30.987257784561212	21.281123209236537
26	20.878378861473358	29.69945160990492	27.823426716805027	21.59874281181669
27	21.526887264808195	28.284126548195786	29.195116935071773	20.993869251924245
28	19.783028632448257	27.27108319976515	32.87425973225239	20.071628435534198
29	20.25769161374058	25.117477615851037	32.53096391577193	22.09386685463646
30	22.147427310659477	26.594627467786967	30.340242428881513	20.91770279267204
31	25.322151317646096	26.011245382592413	26.87218712976098	21.79441617000051
32	26.25660989020001	25.775028420477547	27.087185992100725	20.881175697221714
33	25.166727159630348	25.797045616180768	26.863733535995276	22.172493688193608
34	21.136844336533784	26.64328820404031	29.63403088800166	22.585836571424245
35	21.811281299851494	26.467424013337332	29.41110415290154	22.310190533909633
36	26.095297656924565	26.166122790762618	26.94114069682006	20.797438855492754
37	22.600830134496256	28.84240019817579	27.613769179879437	20.943000487448515
38	22.434639210291348	29.344989479271245	26.413337836308987	21.807033474128428
39	22.346948946602307	28.653224751617856	26.75636027786668	22.243466023913154
40	23.87116134293538	26.428815062705898	26.600767889129223	23.099255705229492
41	20.55892974979465	25.703530363752673	27.39980073071014	26.337739155742533
42	22.701474163756625	26.997834450034848	25.933417644963363	24.367273741245167
43	22.396513922984816	26.761259997636362	27.43977655595543	23.402449523423392
44	22.236169016359174	28.370849485235023	27.558883906922986	21.834097591482816
45	20.94577629435666	29.57046070403716	25.810861564200838	23.672901437405343
46	22.463616952105134	28.46261934392542	27.15441519426232	21.919348509707124
47	22.229586989372443	26.902615861549478	28.088284959290267	22.779512189787805
48	23.22572315027167	25.4810031766166	28.471093966531342	22.822179706580386
49	22.082973915406022	24.785642517286757	29.800200783366353	23.33118278394086
50	20.927880751335227	28.8516528878696	27.651452861541493	22.569013499253685
51	20.44011680259008	29.39672042619571	26.442063232040212	23.721099539173995
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1225.0
1	2518.5
2	3812.0
3	19516.5
4	35221.0
5	26580.0
6	17939.0
7	18020.0
8	18101.0
9	18831.0
10	19561.0
11	19788.0
12	20015.0
13	19706.5
14	19398.0
15	18990.5
16	18583.0
17	18694.0
18	18805.0
19	18369.5
20	17934.0
21	19222.0
22	20510.0
23	20524.0
24	20538.0
25	25654.0
26	35605.5
27	40441.0
28	49259.0
29	58077.0
30	68273.5
31	78470.0
32	91496.0
33	104522.0
34	119330.5
35	134139.0
36	145858.0
37	157577.0
38	179161.5
39	200746.0
40	234367.0
41	267988.0
42	311168.0
43	354348.0
44	368225.5
45	382103.0
46	384532.0
47	386961.0
48	388789.0
49	390617.0
50	376382.0
51	362147.0
52	339857.5
53	317568.0
54	291561.5
55	265555.0
56	246096.0
57	226637.0
58	207616.0
59	188595.0
60	174217.0
61	159839.0
62	141747.5
63	123656.0
64	109068.0
65	94480.0
66	81104.0
67	67728.0
68	56797.5
69	45867.0
70	39138.5
71	32410.0
72	26516.0
73	20622.0
74	17721.5
75	11716.0
76	8611.0
77	6425.0
78	4239.0
79	3188.5
80	2138.0
81	1545.5
82	953.0
83	748.5
84	544.0
85	395.0
86	246.0
87	232.5
88	219.0
89	133.5
90	48.0
91	34.5
92	21.0
93	24.0
94	27.0
95	14.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4755374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.54301923626445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.22726821643067	15.48340026790388
2	7.874750518379628	3.0779280172295307
3	2.5503544574487513	1.495248786636412
4	1.259330319984611	0.9844446667308129
5	0.8026661866235775	0.7843260362741803
6	0.5495022617085151	0.64433599625642
7	0.4370354074267434	0.5978693961988663
8	0.3363111012950544	0.5258027457582826
9	0.28296319251531477	0.4976959603033438
>10	3.7559623152552284	17.976720486537666
>50	1.735822471373569	24.75458772737317
>100	1.183894106653993	32.045057104548505
>500	0.0032679828192169504	0.419856734729314
>1k	6.535965638406274E-4	0.2023921497342161
>5k	1.0893276064010457E-4	0.1223677371065819
>10k+	1.0893276064010457E-4	0.3879661866789055
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18224	0.3832295840453348	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5748	0.12087377354546666	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.042478257230661565	0.0
2	0.0	0.0	0.0	0.13342799115274634	0.0
3	0.0	0.0	0.0	0.21125572878179508	0.0
4	0.0	0.0	0.0	0.34119293245915044	0.0
5	0.0	0.0	0.0	0.6072918765169679	0.0
6	0.0	0.0	0.0	1.0530191736759296	0.0
7	0.0	0.0	0.0	1.3155432148975033	0.0
8	0.0	0.0	0.0	1.9119631810242474	0.0
9	0.0	0.0	0.0	2.2067664919730814	0.0
10	0.0	0.0	0.0	2.562238006936994	0.0
11	0.0	0.0	0.0	2.879920696037788	0.0
12	0.0	0.0	0.0	3.1560714257175144	0.0
13	0.0	0.0	0.0	3.290866291484119	0.0
14	0.0	0.0	0.0	3.3613759927189744	0.0
15	0.0	0.0	0.0	3.438783153543759	0.0
16	0.0	0.0	0.0	3.583040997406303	0.0
17	0.0	0.0	0.0	3.7535218050147057	0.0
18	0.0	0.0	0.0	3.962590534414328	0.0
19	0.0	0.0	0.0	4.088132710487125	0.0
20	0.0	0.0	0.0	4.22372667218183	0.0
21	0.0	0.0	0.0	4.396583738734325	0.0
22	0.0	0.0	0.0	4.585485810369489	0.0
23	0.0	0.0	0.0	4.8026506432511935	0.0
24	0.0	0.0	0.0	4.967012058357555	0.0
25	0.0	0.0	0.0	5.113393815081632	0.0
26	0.0	0.0	0.0	5.2538664677058	0.0
27	0.0	0.0	0.0	5.40178332976544	0.0
28	0.0	0.0	0.0	5.557102343580127	0.0
29	0.0	0.0	0.0	5.727204632064692	0.0
30	2.1028840213198793E-5	0.0	0.0	5.9378294956400905	0.0
31	2.1028840213198793E-5	0.0	0.0	6.1254488080222504	0.0
32	2.1028840213198793E-5	0.0	0.0	6.31674816744172	0.0
33	2.1028840213198793E-5	0.0	0.0	6.502348711163412	0.0
34	2.1028840213198793E-5	0.0	0.0	6.698779107594902	0.0
35	2.1028840213198793E-5	0.0	0.0	6.91478735426488	0.0
36	2.1028840213198793E-5	0.0	0.0	7.111385981418076	0.0
37	2.1028840213198793E-5	0.0	0.0	7.317132154064013	0.0
38	2.1028840213198793E-5	0.0	0.0	7.538797158751341	0.0
39	2.1028840213198793E-5	0.0	0.0	7.831308326116936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	17795	0.0	42.395336	1
GACCGAT	2795	0.0	38.640434	9
AGGGCGA	4935	0.0	38.206688	6
CGTTAGG	695	0.0	37.55396	2
AGTACGG	585	0.0	37.307693	2
GGGCGAT	10440	0.0	36.853447	7
TTGCGAG	625	0.0	36.719997	1
ACGGGAT	1645	0.0	36.38298	5
GCGAGAC	1150	0.0	36.0	21
TATAGCG	325	0.0	36.0	1
TAAGGGA	5250	0.0	35.87143	4
ATAGCGG	1115	0.0	35.11211	2
TAGGGAC	4325	0.0	35.063587	5
TGGGCGA	3545	0.0	35.035263	6
ATAGGGC	2525	0.0	34.930695	4
TACGGGA	1620	0.0	34.72222	4
ACACGAC	1215	0.0	34.62963	26
AGGGATT	6630	0.0	34.615383	6
GGCGATA	2090	0.0	34.557415	8
AGGGACC	4115	0.0	34.501823	6
>>END_MODULE