FastQCFastQC Report
Thu 26 May 2016
SRR1545353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545353_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5915620
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176210.2978724123591441No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC86440.14612162376893714No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT62480.10561868409397493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG3350.042.9850771
CGTTTTT214500.042.6818161
CGAACTA501.0841177E-940.524
CGGGTAT6900.039.1304366
CATACGA10550.038.17535418
AGTCGAC301.1404944E-437.49999617
ACGGGAT18300.037.131155
TGTTACG2000.037.1251
TATTGCG3700.037.0945931
TAGGGCG23700.036.9303785
GGCGATT35950.036.801118
GCGATTT10550.036.4691939
CGAGGGA31450.036.4149444
GGCGATA27200.036.2316178
TGGGCGA36500.036.184936
TACGTAG2800.036.1607171
GGGCGAT126550.036.1102337
AGGGCGA62100.036.0507246
CAACGCG1250.036.0000041
TTGTACG2000.036.01
Produced by FastQC (version 0.11.5)