FastQCFastQC Report
Thu 26 May 2016
SRR1545352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545352_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4679915
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147880.315988645092913No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC77410.16540898712903973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG551.8189894E-1245.01
TCGAACG603.6379788E-1241.2499961
CGAATAT7150.040.2797214
CGTTTTT127000.039.5610241
CGTTAGG5350.039.532712
GCTACGA7350.039.48979610
CCGCGTA403.461264E-739.37500440
GCGATAT5450.039.2201849
CGGTCTA1750.038.57142631
TATCACG659.094947E-1238.076921
CAATACG659.094947E-1238.076921
CACGACC16000.037.82812527
TATCGTG900.037.51
ATACTAT30850.037.48784645
GGCGATA23750.036.852638
GGGCGAT117800.036.8442277
ACACGAC16250.036.8307726
GCGTTAG3950.036.4556961
TATGGGA30250.036.446284
TAGGGTC10950.036.369865
Produced by FastQC (version 0.11.5)