##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545352_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4679915
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.176601711783228	31.0	31.0	33.0	30.0	33.0
2	31.53523429378525	31.0	31.0	34.0	30.0	34.0
3	31.707954524815086	31.0	31.0	34.0	30.0	34.0
4	35.51171677263369	37.0	35.0	37.0	33.0	37.0
5	35.41602913728134	37.0	35.0	37.0	33.0	37.0
6	35.47603621005937	37.0	35.0	37.0	33.0	37.0
7	35.83027255836912	37.0	35.0	37.0	35.0	37.0
8	35.872241696697486	37.0	35.0	37.0	35.0	37.0
9	37.7571616151148	39.0	37.0	39.0	35.0	39.0
10	37.097677842439445	39.0	37.0	39.0	33.0	39.0
11	36.737106336333035	39.0	35.0	39.0	32.0	39.0
12	36.10438629761438	38.0	35.0	39.0	32.0	39.0
13	35.84668866849077	38.0	35.0	39.0	31.0	39.0
14	36.82527866424925	39.0	35.0	41.0	31.0	41.0
15	37.02392650293862	39.0	35.0	41.0	32.0	41.0
16	37.09797742053007	39.0	35.0	41.0	32.0	41.0
17	37.025233577960286	38.0	35.0	41.0	32.0	41.0
18	36.96722718254498	38.0	35.0	41.0	32.0	41.0
19	36.935676823190164	38.0	35.0	41.0	31.0	41.0
20	36.808847596590965	38.0	35.0	41.0	31.0	41.0
21	36.70180355839796	38.0	35.0	41.0	31.0	41.0
22	36.57394247545094	38.0	35.0	41.0	31.0	41.0
23	36.47658600636977	38.0	35.0	40.0	31.0	41.0
24	36.35079654224489	38.0	35.0	40.0	30.0	41.0
25	36.21799220712342	38.0	35.0	40.0	30.0	41.0
26	36.02860244256573	38.0	35.0	40.0	30.0	41.0
27	35.886245156162026	38.0	34.0	40.0	30.0	41.0
28	35.79824206208873	38.0	34.0	40.0	29.0	41.0
29	35.91625574396116	38.0	35.0	40.0	30.0	41.0
30	35.893808327715355	38.0	35.0	40.0	30.0	41.0
31	35.769518463476366	38.0	34.0	40.0	29.0	41.0
32	35.64789745112892	38.0	34.0	40.0	29.0	41.0
33	35.44606814440006	37.0	34.0	40.0	28.0	41.0
34	35.27128783321919	37.0	34.0	40.0	27.0	41.0
35	35.128736098839404	37.0	34.0	40.0	27.0	41.0
36	34.966091050799	37.0	34.0	40.0	26.0	41.0
37	34.855534341969886	37.0	33.0	40.0	25.0	41.0
38	34.78763439079556	37.0	33.0	40.0	26.0	41.0
39	34.70445937586473	37.0	33.0	40.0	25.0	41.0
40	34.56275039183404	36.0	33.0	40.0	24.0	41.0
41	34.49090528353613	36.0	33.0	40.0	24.0	41.0
42	34.413438705617516	36.0	33.0	40.0	24.0	41.0
43	34.280723474678496	36.0	33.0	40.0	24.0	41.0
44	34.17008001213697	36.0	33.0	40.0	23.0	41.0
45	34.12478709549212	35.0	33.0	40.0	23.0	41.0
46	34.11248537633696	35.0	33.0	40.0	23.0	41.0
47	34.07167651549227	35.0	33.0	40.0	23.0	41.0
48	33.96212858566876	35.0	33.0	40.0	23.0	41.0
49	33.9125749078776	35.0	33.0	40.0	24.0	41.0
50	33.78983143924623	35.0	33.0	39.0	24.0	41.0
51	33.55429489638166	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	6.0
11	13.0
12	8.0
13	22.0
14	60.0
15	150.0
16	428.0
17	1123.0
18	2539.0
19	5084.0
20	8442.0
21	13466.0
22	20275.0
23	28965.0
24	40402.0
25	56713.0
26	73767.0
27	86909.0
28	97979.0
29	111893.0
30	132861.0
31	161149.0
32	197812.0
33	254769.0
34	418704.0
35	569861.0
36	367133.0
37	455604.0
38	623304.0
39	949953.0
40	514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.00681422632676	23.799427981063758	27.944524633460222	14.249233159149258
2	30.761092883097234	25.938718972459966	29.92926153573302	13.370926608709773
3	29.217966565632068	25.911218472985087	30.63359056735005	14.237224394032797
4	26.710292815147284	28.19303342047879	29.873170773400798	15.223502990973126
5	23.74867919609651	33.02239463750944	28.96351749978365	14.2654086666104
6	22.545943676327454	41.0681817939001	25.461488082582694	10.924386447189747
7	88.56536924281745	3.8938741408764903	5.733736617011206	1.807019999294859
8	89.34360987325624	3.085141503638421	5.563989944261808	2.007258678843526
9	84.4366190411578	4.919020965124367	7.413681658748075	3.2306783349697588
10	47.96773018313367	24.046035024140398	14.47859629929176	13.507638493434177
11	39.28218781751378	23.642822572632195	20.085407534111198	16.989582075742828
12	34.49366067546099	21.90003450917378	25.90841927684584	17.697885538519394
13	23.713507617125526	31.711601599601703	26.323213135281303	18.25167764799147
14	20.282911121248997	33.3366524819361	28.022367927622614	18.358068469192283
15	19.515183502264463	23.48679409775605	38.6942497887248	18.303772611254693
16	20.981769968044294	20.005064194541994	37.22088969564618	21.792276141767534
17	20.994825760724286	20.500543279098018	28.536586668774966	29.96804429140273
18	24.14990443202494	23.02067452079792	30.26193851811411	22.567482529063028
19	28.720137011035458	24.410592927435648	24.235311966136138	22.63395809539276
20	30.610385017676606	23.16604040885358	24.324181101579835	21.89939347188998
21	26.057118558777244	25.54683151296551	25.79012653007587	22.60592339818138
22	25.528647422015144	24.573202718425442	24.386596765112188	25.511553094447226
23	24.070672223747653	27.864459076714	24.41845631811689	23.64641238142146
24	23.665216141746164	24.375998282020078	31.501982407800146	20.456803168433616
25	22.864517838465016	25.199474776785475	28.799817945411398	23.13618943933811
26	21.576139737580704	29.143926759353533	25.564395934541544	23.715537568524216
27	22.580324642648424	27.458255117881414	26.95933579990235	23.002084439567813
28	20.037116058731836	26.851620168315023	31.341936765945533	21.769327007007604
29	21.033458941027774	24.298240459495524	31.059410267066816	23.608890332409885
30	22.974007006537512	26.326332850062446	28.999971153322228	21.69968899007781
31	27.434515370471473	25.189581434705545	24.802053028740907	22.573850166082078
32	28.83024584848229	24.75269315788855	24.568330835068586	21.848730158560574
33	26.936151618138364	25.60809330938703	24.691409993557574	22.76434507891703
34	22.213309429765285	25.553092310437265	28.116514936702906	24.117083323094544
35	23.7669060228658	24.869191000263893	28.34799349988194	23.015909476988362
36	28.213995339658947	26.152889528976488	24.915708939158083	20.717406192206482
37	23.836544039795594	28.69926056349314	26.621274104337367	20.8429212923739
38	23.798316849771844	28.881998925194154	24.594506524156955	22.725177700877044
39	23.545598584589676	28.551843356129332	25.424350655941403	22.47820740333959
40	25.006330243177494	25.95104825621833	25.506553003633613	23.536068496970564
41	21.593383640514837	24.58140799565804	26.050815025486575	27.77439333834055
42	23.632373664906307	26.854205685359673	23.847334834072843	25.666085815661184
43	23.67602830393287	26.51734059272444	25.329562609577312	24.477068493765376
44	22.721203269717506	28.62398141846593	25.501040082992958	23.1537752288236
45	21.236582288353528	29.238800277355466	24.260483363479892	25.264134070811117
46	23.406343918639546	28.477995861035936	25.924958038767798	22.190702181556716
47	23.907164980560545	25.675487695823534	27.111261636162194	23.306085687453727
48	24.72626105388666	23.58408218952695	27.96352070497007	23.726136051616322
49	22.616564617092404	24.708653896491708	28.406584307620967	24.268197178794914
50	21.64329907701315	27.87894651932781	26.940296992573582	23.53745741108546
51	21.468958303729877	28.313655269379893	25.437556023987618	24.77983040290262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1237.0
1	3239.0
2	5241.0
3	16988.5
4	28736.0
5	21395.0
6	14054.0
7	13830.0
8	13606.0
9	14392.0
10	15178.0
11	15510.0
12	15842.0
13	16099.0
14	16356.0
15	15816.5
16	15277.0
17	14934.0
18	14591.0
19	14601.5
20	14612.0
21	15002.5
22	15393.0
23	16343.5
24	17294.0
25	18244.0
26	23261.0
27	27328.0
28	37540.0
29	47752.0
30	53852.5
31	59953.0
32	71155.0
33	82357.0
34	92231.0
35	102105.0
36	112742.5
37	123380.0
38	136606.5
39	149833.0
40	190559.5
41	231286.0
42	275961.0
43	320636.0
44	331681.0
45	342726.0
46	347693.0
47	352660.0
48	352241.5
49	351823.0
50	346187.0
51	340551.0
52	327113.0
53	313675.0
54	293351.0
55	273027.0
56	268237.5
57	263448.0
58	249063.5
59	234679.0
60	224601.0
61	214523.0
62	198154.5
63	181786.0
64	160230.0
65	138674.0
66	117590.0
67	96506.0
68	85399.0
69	74292.0
70	63836.5
71	53381.0
72	45990.5
73	38600.0
74	32440.5
75	20617.0
76	14953.0
77	11629.0
78	8305.0
79	6523.5
80	4742.0
81	3648.5
82	2555.0
83	1779.5
84	1004.0
85	638.0
86	272.0
87	184.5
88	97.0
89	69.5
90	42.0
91	34.5
92	27.0
93	24.5
94	22.0
95	18.5
96	15.0
97	11.5
98	8.0
99	4.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4679915.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.158766137864323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87201099393457	14.503771685997858
2	8.046490498126053	2.9222867837203683
3	2.5865180175524456	1.4090392737632191
4	1.2366598117660295	0.8982486525581858
5	0.7057653406439149	0.6407913884481501
6	0.5425631099168755	0.591136597680775
7	0.38162503970408707	0.48508878938377925
8	0.28023098405256797	0.4070919123195334
9	0.24615041810553798	0.4022809089404389
>10	3.023139972640339	13.263292494672497
>50	1.4696094607245227	19.59674839648822
>100	1.601170230162639	43.05640273968705
>500	0.0059309725518200355	0.7328495921227027
>1k	0.001897911216582377	0.6056999832610327
>5k	1.1861945103639856E-4	0.16673981402644575
>10k+	1.1861945103639856E-4	0.31853098692973514
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14788	0.315988645092913	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7741	0.16540898712903973	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03908190640214619	0.0
2	0.0	0.0	0.0	0.10835666887112266	0.0
3	0.0	0.0	0.0	0.17925539245905106	0.0
4	0.0	0.0	0.0	0.2987447421587785	0.0
5	0.0	0.0	0.0	0.5081075190468203	0.0
6	0.0	0.0	0.0	0.8681781613554947	0.0
7	0.0	0.0	0.0	1.0728399981623598	0.0
8	0.0	0.0	0.0	1.5540239512897136	0.0
9	0.0	0.0	0.0	1.8069558955664793	0.0
10	0.0	0.0	0.0	2.1376670302772594	0.0
11	0.0	0.0	0.0	2.452352232893119	0.0
12	0.0	0.0	0.0	2.720562232433709	0.0
13	0.0	0.0	0.0	2.849966292122827	0.0
14	0.0	0.0	0.0	2.9111853527254232	0.0
15	0.0	0.0	0.0	2.9863576582053306	0.0
16	0.0	0.0	0.0	3.1250781264189627	0.0
17	0.0	0.0	0.0	3.2831792885127187	0.0
18	0.0	0.0	0.0	3.4796572159964443	0.0
19	0.0	0.0	0.0	3.592479777944685	2.1367909459894035E-5
20	0.0	0.0	0.0	3.7168410110012684	2.1367909459894035E-5
21	0.0	0.0	0.0	3.868083074158398	2.1367909459894035E-5
22	0.0	0.0	0.0	4.043513610824128	2.1367909459894035E-5
23	0.0	2.1367909459894035E-5	0.0	4.235333334045597	2.1367909459894035E-5
24	0.0	2.1367909459894035E-5	0.0	4.392558411851497	2.1367909459894035E-5
25	0.0	2.1367909459894035E-5	0.0	4.523821479663626	2.1367909459894035E-5
26	0.0	2.1367909459894035E-5	0.0	4.642285169709279	2.1367909459894035E-5
27	0.0	2.1367909459894035E-5	0.0	4.765578007292867	2.1367909459894035E-5
28	0.0	2.1367909459894035E-5	0.0	4.898678715318548	2.1367909459894035E-5
29	0.0	2.1367909459894035E-5	0.0	5.0470574786080515	2.1367909459894035E-5
30	0.0	2.1367909459894035E-5	0.0	5.237616495171387	2.1367909459894035E-5
31	0.0	2.1367909459894035E-5	0.0	5.3977262407543725	2.1367909459894035E-5
32	0.0	2.1367909459894035E-5	0.0	5.5613616913982415	2.1367909459894035E-5
33	0.0	2.1367909459894035E-5	0.0	5.718351722200083	2.1367909459894035E-5
34	0.0	2.1367909459894035E-5	0.0	5.889444573245454	2.1367909459894035E-5
35	0.0	2.1367909459894035E-5	0.0	6.084234435881848	2.1367909459894035E-5
36	0.0	2.1367909459894035E-5	0.0	6.256075163758316	2.1367909459894035E-5
37	0.0	2.1367909459894035E-5	0.0	6.425437214137436	2.1367909459894035E-5
38	0.0	2.1367909459894035E-5	0.0	6.6084533586614285	2.1367909459894035E-5
39	0.0	2.1367909459894035E-5	0.0	6.820145237680599	2.1367909459894035E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	55	1.8189894E-12	45.0	1
TCGAACG	60	3.6379788E-12	41.249996	1
CGAATAT	715	0.0	40.27972	14
CGTTTTT	12700	0.0	39.561024	1
CGTTAGG	535	0.0	39.53271	2
GCTACGA	735	0.0	39.489796	10
CCGCGTA	40	3.461264E-7	39.375004	40
GCGATAT	545	0.0	39.220184	9
CGGTCTA	175	0.0	38.571426	31
TATCACG	65	9.094947E-12	38.07692	1
CAATACG	65	9.094947E-12	38.07692	1
CACGACC	1600	0.0	37.828125	27
TATCGTG	90	0.0	37.5	1
ATACTAT	3085	0.0	37.487846	45
GGCGATA	2375	0.0	36.85263	8
GGGCGAT	11780	0.0	36.844227	7
ACACGAC	1625	0.0	36.83077	26
GCGTTAG	395	0.0	36.455696	1
TATGGGA	3025	0.0	36.44628	4
TAGGGTC	1095	0.0	36.36986	5
>>END_MODULE