FastQCFastQC Report
Thu 26 May 2016
SRR1545351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545351_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2931314
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC77400.2640454076226566No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57870.19741999662949788No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGA207.035078E-445.00000416
TCCGATT253.8922914E-545.017
CACGTAT253.8922914E-545.038
TAATGCG600.044.9999961
CACGACC3700.041.35135327
GTTAGCG2050.040.6097561
TAGTTCG451.9301297E-840.01
CTATACG850.039.7058831
ACGTAAG850.039.7058831
CGACTCG403.4606637E-739.37500421
CGAGGGT4500.039.04
CGTAAGG3750.039.02
TACGGGA6500.038.769234
GACCGAT17600.038.7357949
GGCGATT17200.038.590128
CGTTAGG4900.038.571432
CGACTAT356.2516046E-638.57142621
TAGTCGA356.2516046E-638.57142619
GGGCGAT63450.038.4751787
TATACTA18950.038.46965844
Produced by FastQC (version 0.11.5)