##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545351_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2931314
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.170914477261732	31.0	31.0	33.0	30.0	33.0
2	31.52342976562729	31.0	31.0	34.0	30.0	34.0
3	31.717757974751255	31.0	31.0	34.0	30.0	34.0
4	35.51172034111664	37.0	35.0	37.0	33.0	37.0
5	35.42783748175733	37.0	35.0	37.0	33.0	37.0
6	35.47914280080537	37.0	35.0	37.0	33.0	37.0
7	35.80653556732578	37.0	35.0	37.0	35.0	37.0
8	35.845784518478744	37.0	35.0	37.0	35.0	37.0
9	37.73882122488413	39.0	37.0	39.0	35.0	39.0
10	37.10841042617748	39.0	37.0	39.0	33.0	39.0
11	36.7166721135982	39.0	35.0	39.0	32.0	39.0
12	35.86719437085212	37.0	35.0	39.0	31.0	39.0
13	35.47320655514899	37.0	35.0	39.0	30.0	39.0
14	36.41783548265386	38.0	35.0	41.0	31.0	41.0
15	36.7155166590819	38.0	35.0	41.0	31.0	41.0
16	36.85237746621481	38.0	35.0	41.0	32.0	41.0
17	36.79618355454243	38.0	35.0	41.0	32.0	41.0
18	36.76417981833403	38.0	35.0	40.0	32.0	41.0
19	36.71924160973543	38.0	35.0	40.0	31.0	41.0
20	36.598576611035185	38.0	35.0	40.0	31.0	41.0
21	36.45211737807686	38.0	35.0	40.0	31.0	41.0
22	36.32953515044789	38.0	35.0	40.0	31.0	41.0
23	36.255069569483176	38.0	35.0	40.0	31.0	41.0
24	36.147934680488	37.0	35.0	40.0	31.0	41.0
25	36.028152562298	37.0	35.0	40.0	30.0	41.0
26	35.858982695132624	37.0	34.0	40.0	30.0	41.0
27	35.71546037033221	37.0	34.0	40.0	30.0	41.0
28	35.67254992129809	37.0	34.0	40.0	30.0	41.0
29	35.79516148730569	37.0	34.0	40.0	30.0	41.0
30	35.7855896707074	37.0	34.0	40.0	30.0	41.0
31	35.663587387772175	37.0	34.0	40.0	30.0	41.0
32	35.51514542624911	37.0	34.0	40.0	29.0	41.0
33	35.332157523895425	37.0	34.0	40.0	29.0	41.0
34	35.19879958271274	36.0	34.0	40.0	28.0	41.0
35	35.0831030043182	36.0	34.0	40.0	27.0	41.0
36	34.894456888617185	36.0	34.0	40.0	26.0	41.0
37	34.78272644963999	36.0	33.0	40.0	26.0	41.0
38	34.739965762794434	36.0	33.0	40.0	26.0	41.0
39	34.70371478456419	36.0	33.0	40.0	26.0	41.0
40	34.555967733241815	36.0	33.0	40.0	25.0	41.0
41	34.49226114977788	36.0	33.0	40.0	25.0	41.0
42	34.435534030131194	35.0	33.0	40.0	25.0	41.0
43	34.29837847463629	35.0	33.0	40.0	25.0	41.0
44	34.1722060482091	35.0	33.0	40.0	24.0	41.0
45	34.107252242509674	35.0	33.0	40.0	24.0	41.0
46	34.123843095621964	35.0	33.0	40.0	24.0	41.0
47	34.07986725407104	35.0	33.0	39.0	24.0	41.0
48	34.0314814448401	35.0	33.0	39.0	24.0	41.0
49	33.996325879793154	35.0	33.0	39.0	24.0	41.0
50	33.84905608883934	35.0	33.0	39.0	24.0	41.0
51	33.59516755966778	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	10.0
13	10.0
14	32.0
15	73.0
16	209.0
17	573.0
18	1338.0
19	2647.0
20	4630.0
21	7538.0
22	11690.0
23	16660.0
24	23391.0
25	32593.0
26	43138.0
27	52309.0
28	60518.0
29	70528.0
30	85094.0
31	104983.0
32	131072.0
33	170190.0
34	295403.0
35	417459.0
36	219264.0
37	270138.0
38	367629.0
39	541881.0
40	307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.04479561043273	23.31490246353683	28.632449474877138	13.007852451153306
2	30.694528119471336	26.038220402181413	29.69364592124897	13.573605557098285
3	29.819323347822852	25.373842583906058	30.18120883671964	14.625625231551448
4	26.82523946598693	27.689493517241754	29.609315139899717	15.8759518768716
5	23.875436067238105	32.3598904791503	28.861698200875104	14.902975252736486
6	22.608870970493097	41.04289066268574	25.12235127318329	11.225887093637871
7	88.83476829844909	4.07663593869507	5.241744828428479	1.8468509344273594
8	90.12654393217512	3.0710459541352444	4.835476513263335	1.9669336004262936
9	85.61160626258395	4.646209856740015	6.851534840689193	2.8906490399868456
10	53.2853184612771	21.343670449498077	12.724941783787067	12.646069305437765
11	44.84053908929579	21.737930498063328	17.929843066965873	15.491687345675013
12	39.335772285057146	21.764983212306834	23.12065510552605	15.778589397109965
13	22.950321937533815	37.35607990136847	23.45228112716686	16.24131703393086
14	18.1350411453703	38.49846860486458	26.192281004355046	17.17420924541008
15	17.1774501128163	22.801719638360137	43.933130329947595	16.087699918875973
16	18.501054475910802	18.432757459623907	42.53406492787876	20.532123136586527
17	19.097851680168006	18.77659643422711	27.268180754432997	34.85737113117189
18	23.795471928288812	21.6790149400576	31.87846815455458	22.647044977099007
19	30.26171880596893	24.05422960488027	23.776947812482728	21.907103776668073
20	33.07202844867523	22.251352123996266	23.19198830285667	21.48463112447182
21	25.268974937519488	26.534925975177003	25.564200901029366	22.631898186274142
22	25.12351116257078	23.69555086899595	23.651372729090095	27.529565239343174
23	22.739870242491932	29.178109202903546	23.301120248461952	24.78090030614257
24	22.783843696035294	23.701964375020896	34.06236929922895	19.451822629714865
25	20.388058051781556	24.63765396678759	32.16670066734577	22.807587314085083
26	20.748408392959607	31.279521743491145	25.44664270016791	22.525427163381337
27	21.462149738990774	30.24067022502536	26.803099224443372	21.49408081154049
28	18.31758726632493	27.163927167133924	34.11459843605974	20.403887130481415
29	18.9802593649128	23.547801429666013	34.05888963106648	23.41304957435471
30	21.48156082903435	28.06724219923215	28.9580031344305	21.493193837303
31	30.055974897264505	25.387351883830938	22.595088755418217	21.961584463486343
32	30.827335454338904	25.293093813900526	23.924253764693923	19.955316967066647
33	28.581039083496346	26.246454661629564	23.77142128069528	21.401084974178815
34	21.61685851464565	25.58002997972923	27.4647820056125	25.338329500012623
35	22.695112157892332	24.89552466914155	28.92057964448708	23.488783528479036
36	30.84401739288251	24.211360502491374	24.481239471445228	20.46338263318089
37	23.484928601985324	29.86107936577248	26.506474570789756	20.14751746145244
38	22.66266936943637	30.731712808658507	22.82212004582245	23.783497776082672
39	22.176198114565686	30.19649208511951	24.741600524542918	22.88570927577189
40	25.34068339318135	24.940043952984908	23.891469832300462	25.82780282153328
41	19.42944358741506	23.98204354770591	25.872185647801633	30.716327217077392
42	22.94882090420883	25.954537794313403	23.65512531240256	27.441515989075207
43	23.483222882297834	25.551169202616986	25.242433939182224	25.72317397590296
44	21.89434499340569	28.415686616991557	26.754042726231308	22.935925663371442
45	20.30321555452606	32.37080708515021	22.788994969491497	24.536982390832236
46	23.023633769701917	29.877829533103583	25.456467645567827	21.642069051626677
47	23.54053506379733	26.208724142142398	26.320551124853903	23.93018966920637
48	24.430886626270677	22.39551955198249	28.997916975117644	24.17567684662919
49	21.546958121852523	22.672255514080035	30.629233169834414	25.151553194233028
50	20.671139291116543	30.449075056442265	26.107267935130796	22.772517717310397
51	20.361107680719297	31.710932366849814	23.74317456267053	24.18478538976036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	550.0
1	1874.5
2	3199.0
3	7757.0
4	12315.0
5	9621.5
6	6928.0
7	7053.0
8	7178.0
9	7506.0
10	7834.0
11	8111.0
12	8388.0
13	8303.5
14	8219.0
15	8092.0
16	7965.0
17	7803.0
18	7641.0
19	7166.5
20	6692.0
21	7112.5
22	7533.0
23	8739.0
24	9945.0
25	10550.0
26	13314.5
27	15474.0
28	20548.5
29	25623.0
30	29048.5
31	32474.0
32	39286.5
33	46099.0
34	53785.5
35	61472.0
36	65886.0
37	70300.0
38	84132.0
39	97964.0
40	135107.0
41	172250.0
42	207672.5
43	243095.0
44	242835.5
45	242576.0
46	235291.0
47	228006.0
48	221923.0
49	215840.0
50	216256.0
51	216672.0
52	212546.0
53	208420.0
54	192779.0
55	177138.0
56	160761.0
57	144384.0
58	142525.0
59	140666.0
60	131585.0
61	122504.0
62	114178.5
63	105853.0
64	94303.5
65	82754.0
66	71791.5
67	60829.0
68	51901.0
69	42973.0
70	35671.0
71	28369.0
72	23207.5
73	18046.0
74	14740.5
75	9686.0
76	7937.0
77	5830.5
78	3724.0
79	2584.5
80	1445.0
81	1177.0
82	909.0
83	655.5
84	402.0
85	238.0
86	74.0
87	58.0
88	42.0
89	29.5
90	17.0
91	10.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2931314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.460392755513226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40902868512855	13.23564193246545
2	7.860738651674826	2.5878169111002216
3	2.3730490462523623	1.171839579882299
4	1.0936389215829263	0.7200690472788358
5	0.6427107029858355	0.5289635299659429
6	0.44271041918843784	0.4372312426079752
7	0.29504345915150515	0.33995718523052987
8	0.2550897104098044	0.33591014569884087
9	0.21551984631528678	0.3192787185261723
>10	2.7356530697939707	11.496489658474381
>50	1.7388892254565282	21.340528719817286
>100	1.9297813185809327	46.094330101844314
>500	0.0068935675592254955	0.7073702741548225
>1k	8.355839465727874E-4	0.2194457072553022
>5k	4.177919732863937E-4	0.46512724569766106
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7740	0.2640454076226566	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5787	0.19741999662949788	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04325705127461609	0.0
2	0.0	0.0	0.0	0.14853407038618177	0.0
3	0.0	0.0	0.0	0.21297616017935983	0.0
4	0.0	0.0	0.0	0.34776212988441363	0.0
5	0.0	0.0	0.0	0.5913389012572519	0.0
6	0.0	0.0	0.0	0.8984707881857761	0.0
7	0.0	0.0	0.0	1.0544417964093917	0.0
8	0.0	0.0	0.0	1.457571587349564	0.0
9	0.0	0.0	0.0	1.6099605842294615	0.0
10	0.0	0.0	0.0	1.8528209533335562	0.0
11	0.0	0.0	0.0	2.1182309367062007	0.0
12	0.0	0.0	0.0	2.3335951044480394	0.0
13	0.0	0.0	0.0	2.4256016243909726	0.0
14	0.0	0.0	0.0	2.463639173421885	0.0
15	0.0	0.0	0.0	2.5260343995900816	0.0
16	0.0	0.0	0.0	2.666483358657585	0.0
17	0.0	0.0	0.0	2.8263092933749165	0.0
18	0.0	0.0	0.0	3.0347823535793164	0.0
19	0.0	0.0	0.0	3.1342599257534336	0.0
20	0.0	0.0	0.0	3.2383429410837596	0.0
21	0.0	0.0	0.0	3.377427324401275	0.0
22	0.0	0.0	0.0	3.5222088114749903	0.0
23	0.0	0.0	0.0	3.698614341554675	0.0
24	0.0	0.0	0.0	3.8280443514410263	0.0
25	0.0	0.0	0.0	3.939700762183785	0.0
26	0.0	0.0	0.0	4.042589773732872	0.0
27	0.0	0.0	0.0	4.144591811044466	0.0
28	0.0	0.0	0.0	4.2548154172497386	0.0
29	0.0	0.0	0.0	4.369917381761217	0.0
30	0.0	0.0	0.0	4.520600658953629	0.0
31	0.0	0.0	0.0	4.663301168008613	0.0
32	0.0	0.0	0.0	4.795153299851193	0.0
33	0.0	0.0	0.0	4.926664287756276	0.0
34	0.0	0.0	0.0	5.06591924304254	0.0
35	0.0	0.0	0.0	5.250478113228402	0.0
36	0.0	0.0	0.0	5.398943954827084	0.0
37	0.0	0.0	0.0	5.544987674469538	0.0
38	3.4114393749697234E-5	0.0	0.0	5.693282944099472	0.0
39	3.4114393749697234E-5	0.0	0.0	5.835983453154456	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGA	20	7.035078E-4	45.000004	16
TCCGATT	25	3.8922914E-5	45.0	17
CACGTAT	25	3.8922914E-5	45.0	38
TAATGCG	60	0.0	44.999996	1
CACGACC	370	0.0	41.351353	27
GTTAGCG	205	0.0	40.609756	1
TAGTTCG	45	1.9301297E-8	40.0	1
CTATACG	85	0.0	39.705883	1
ACGTAAG	85	0.0	39.705883	1
CGACTCG	40	3.4606637E-7	39.375004	21
CGAGGGT	450	0.0	39.0	4
CGTAAGG	375	0.0	39.0	2
TACGGGA	650	0.0	38.76923	4
GACCGAT	1760	0.0	38.735794	9
GGCGATT	1720	0.0	38.59012	8
CGTTAGG	490	0.0	38.57143	2
CGACTAT	35	6.2516046E-6	38.571426	21
TAGTCGA	35	6.2516046E-6	38.571426	19
GGGCGAT	6345	0.0	38.475178	7
TATACTA	1895	0.0	38.469658	44
>>END_MODULE