FastQCFastQC Report
Thu 26 May 2016
SRR1545350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545350_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences46525
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.2815690488984417No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC600.1289629231595916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTC351.17865056E-745.0000046
AGCCCAC206.963608E-445.011
ACTAATC406.5756467E-945.010
GTAGACA206.963608E-445.042
GGATGAC206.963608E-445.08
GTCATGA206.963608E-445.024
TATACTA406.5756467E-945.044
GGCACCG253.8331687E-545.08
CCTACAC406.5756467E-945.028
TGCAAGG253.8331687E-545.02
TGTCTGG206.963608E-445.02
TCAGGGT206.963608E-445.04
GGACTCA206.963608E-445.08
AAATATA406.5756467E-945.035
AGAGGGT206.963608E-445.04
AATTATA406.5756467E-945.041
TGACAGG206.963608E-445.02
AGGGTAG206.963608E-445.06
GAGCCCA206.963608E-445.010
AAGGGCG253.8331687E-545.05