##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545350_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	46525
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16973670069855	31.0	31.0	33.0	30.0	33.0
2	31.543965609887156	31.0	31.0	34.0	30.0	34.0
3	31.719656098871575	31.0	31.0	34.0	30.0	34.0
4	35.53695862439549	37.0	35.0	37.0	33.0	37.0
5	35.42693175711983	37.0	35.0	37.0	33.0	37.0
6	35.49659322944653	37.0	35.0	37.0	33.0	37.0
7	35.81472326706072	37.0	35.0	37.0	35.0	37.0
8	35.85508866200967	37.0	35.0	37.0	35.0	37.0
9	37.74067705534659	39.0	37.0	39.0	35.0	39.0
10	37.12367544331005	39.0	37.0	39.0	33.0	39.0
11	36.737216550241804	39.0	35.0	39.0	32.0	39.0
12	36.09442235357335	38.0	35.0	39.0	32.0	39.0
13	35.839376679204726	38.0	35.0	39.0	31.0	39.0
14	36.82448146157979	39.0	35.0	41.0	31.0	41.0
15	37.012079527135946	39.0	35.0	41.0	32.0	41.0
16	37.087802256851155	39.0	35.0	41.0	32.0	41.0
17	37.02207415368082	38.0	35.0	41.0	32.0	41.0
18	36.94033315421816	38.0	35.0	41.0	32.0	41.0
19	36.91503492745836	38.0	35.0	41.0	31.0	41.0
20	36.7905427189683	38.0	35.0	41.0	31.0	41.0
21	36.67645351961311	38.0	35.0	41.0	31.0	41.0
22	36.57164965072542	38.0	35.0	40.0	31.0	41.0
23	36.44453519613111	38.0	35.0	40.0	31.0	41.0
24	36.31692638366469	38.0	35.0	40.0	30.0	41.0
25	36.172358946802795	38.0	35.0	40.0	30.0	41.0
26	35.96195593766792	38.0	35.0	40.0	30.0	41.0
27	35.82854379365932	38.0	34.0	40.0	30.0	41.0
28	35.72833960236432	37.0	34.0	40.0	29.0	41.0
29	35.8896937130575	38.0	35.0	40.0	30.0	41.0
30	35.865964535196134	38.0	35.0	40.0	30.0	41.0
31	35.80827512090274	38.0	34.0	40.0	30.0	41.0
32	35.64221386351424	38.0	34.0	40.0	29.0	41.0
33	35.476990865126275	37.0	34.0	40.0	29.0	41.0
34	35.30648038688877	37.0	34.0	40.0	27.0	41.0
35	35.17676518001075	37.0	34.0	40.0	27.0	41.0
36	34.99963460505105	37.0	34.0	40.0	26.0	41.0
37	34.917958087049975	37.0	34.0	40.0	26.0	41.0
38	34.840515851692636	37.0	33.0	40.0	26.0	41.0
39	34.77983879634605	36.0	33.0	40.0	26.0	41.0
40	34.62026867275658	36.0	33.0	40.0	25.0	41.0
41	34.52507254164428	36.0	33.0	40.0	25.0	41.0
42	34.45603439011284	36.0	33.0	40.0	25.0	41.0
43	34.33803331542182	36.0	33.0	40.0	24.0	41.0
44	34.1993551853842	35.0	33.0	40.0	24.0	41.0
45	34.14766254701773	35.0	33.0	40.0	23.0	41.0
46	34.1394734013971	35.0	33.0	40.0	24.0	41.0
47	34.095239118753355	35.0	33.0	40.0	24.0	41.0
48	34.00414830736163	35.0	33.0	40.0	23.0	41.0
49	33.91907576571736	35.0	33.0	39.0	24.0	41.0
50	33.79946265448684	35.0	33.0	39.0	24.0	41.0
51	33.571413218699625	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	0.0
16	6.0
17	7.0
18	32.0
19	49.0
20	84.0
21	135.0
22	189.0
23	260.0
24	403.0
25	477.0
26	698.0
27	881.0
28	969.0
29	1069.0
30	1355.0
31	1619.0
32	1982.0
33	2570.0
34	4252.0
35	5784.0
36	3714.0
37	4665.0
38	6215.0
39	9106.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.961848468565286	23.439011284255777	28.541644277270283	13.05749596990865
2	31.20902740462117	25.581945190757654	29.708758731864588	13.500268672756583
3	29.90435249865664	25.734551316496507	30.16012896292316	14.200967221923696
4	26.645889306824287	28.51370231058571	29.38420204191295	15.456206340677056
5	24.206340677055348	32.47931219774315	28.31166039763568	15.002686727565825
6	23.101558301988177	41.375604513702314	25.00376141859216	10.519075765717357
7	89.0918860827512	3.9613111230521225	5.250940354648039	1.69586243954863
8	89.93014508328856	2.972595378828587	5.218699623858141	1.8785599140247178
9	85.16066630843633	4.75873186458893	7.013433637829124	3.0671681891456206
10	49.96238581407845	23.174637291778613	13.111230521225147	13.751746372917786
11	40.391187533584095	22.94465341214401	19.85169263836647	16.812466415905426
12	35.344438473938744	21.803331542181624	25.281031703385278	17.571198280494357
13	23.890381515314346	31.303600214938204	26.108543793659322	18.697474476088125
14	19.761418592154754	32.93927995701236	27.64535196131112	19.653949489521764
15	20.18054809242343	22.031166039763566	39.08006448146158	18.708221386351422
16	21.900053734551317	18.63084363245567	38.224610424502956	21.24449220849006
17	22.018269747447608	19.905427189682968	27.752821063944115	30.32348199892531
18	25.263836646964	22.488984416980117	29.457281031703385	22.7898979043525
19	29.618484685652874	23.909725953788286	23.733476625470175	22.738312735088662
20	31.72917786136486	22.540569586243954	24.068780225685117	21.661472326706072
21	26.76410531972058	25.008060182697474	25.652874798495436	22.574959699086513
22	26.078452444922085	23.791509940891995	23.849543256313808	26.280494357872115
23	24.526598602901668	27.602364320257927	23.598065556152605	24.272971520687804
24	24.45996775926921	23.013433637829124	31.636754433100485	20.889844169801183
25	23.57012358946803	23.484148307361632	29.076840408382594	23.868887694787748
26	22.620096722192372	28.208490059108005	25.162815690488983	24.00859752821064
27	23.20687802256851	27.23481998925309	26.76410531972058	22.79419666845782
28	21.23804406233208	26.256851155292853	31.52713594841483	20.977968833960237
29	22.00752283718431	24.423428264373992	29.966684578183774	23.602364320257927
30	23.761418592154758	25.977431488447074	27.65394948952176	22.60720042987641
31	28.747984954325634	24.77592692101021	23.6732939279957	22.802794196668458
32	29.321869962385815	25.43793659322945	24.28586781300376	20.954325631380975
33	27.868887694787748	25.40784524449221	24.341751746372918	22.381515314347126
34	23.07791509940892	25.594841483073616	27.314347125201504	24.012896292315958
35	23.813003761418592	24.64911337990328	28.023643202579258	23.51423965609887
36	28.337452982267596	25.575497044599675	24.582482536270824	21.5045674368619
37	24.6211714132187	28.874798495432564	25.73240193444385	20.77162815690489
38	23.808704997313274	29.2315959161741	24.012896292315958	22.946802794196667
39	24.053734551316495	28.279419666845783	24.640515851692637	23.026329930145085
40	24.90274046211714	25.2745835572273	25.22944653412144	24.59322944653412
41	21.184309511015584	24.073078989790435	25.788285867813006	28.95432563138098
42	23.565824825362707	25.84416980118216	23.583019881783986	27.00698549167114
43	23.65394948952176	25.927995701235893	25.10908113917249	25.308973670069857
44	22.865126276195593	27.50994089199355	25.36055883933369	24.26437399247716
45	21.919398173025257	29.60558839333692	23.7270284793122	24.74798495432563
46	24.047286405158516	28.09887157442235	25.644277270284793	22.209564750134337
47	24.498656636217085	25.75174637291779	26.372917786136483	23.37667920472864
48	24.965072541644275	23.142396560988715	27.501343363782908	24.391187533584095
49	22.75980655561526	23.387426114991943	28.868350349274586	24.984416980118215
50	21.440085975282106	27.81515314347125	27.000537345513166	23.74422353573348
51	21.723804406233207	28.662009672219234	24.631918323482	24.982267598065555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	39.0
2	71.0
3	171.0
4	271.0
5	204.0
6	137.0
7	135.5
8	134.0
9	131.5
10	129.0
11	121.5
12	114.0
13	115.0
14	116.0
15	130.5
16	145.0
17	122.5
18	100.0
19	103.0
20	106.0
21	105.0
22	104.0
23	126.5
24	149.0
25	172.5
26	238.0
27	280.0
28	321.5
29	363.0
30	439.0
31	515.0
32	581.0
33	647.0
34	818.0
35	989.0
36	1102.5
37	1216.0
38	1337.5
39	1459.0
40	1917.0
41	2375.0
42	2825.5
43	3276.0
44	3324.5
45	3373.0
46	3325.5
47	3278.0
48	3260.5
49	3243.0
50	3200.5
51	3158.0
52	2995.5
53	2833.0
54	2819.0
55	2805.0
56	2646.5
57	2488.0
58	2578.0
59	2668.0
60	2517.0
61	2366.0
62	2255.5
63	2145.0
64	1866.0
65	1587.0
66	1374.5
67	1162.0
68	1030.5
69	899.0
70	743.5
71	588.0
72	509.5
73	431.0
74	351.5
75	207.0
76	142.0
77	110.5
78	79.0
79	69.5
80	60.0
81	43.5
82	27.0
83	20.0
84	13.0
85	9.0
86	5.0
87	3.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	46525.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.04997313272433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.87674306779932	47.522837184309516
2	17.45792594967142	23.411069317571197
3	7.273601538708125	14.63084363245567
4	2.779291553133515	7.454056958624395
5	0.9520756531495432	3.1918323481998927
6	0.384677031575573	1.5475550779150995
7	0.12181439333226478	0.5717356260075228
8	0.03205641929796442	0.17195056421278881
9	0.0384677031575573	0.2321332616872649
>10	0.0769354063151146	0.8554540569586244
>50	0.0032056419297964416	0.1289629231595916
>100	0.0032056419297964416	0.2815690488984417
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131	0.2815690488984417	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	60	0.1289629231595916	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03653949489521763	0.0
2	0.0	0.0	0.0	0.12681354110693177	0.0
3	0.0	0.0	0.0	0.199892530897367	0.0
4	0.0	0.0	0.0	0.29661472326706073	0.0
5	0.0	0.0	0.0	0.4986566362170876	0.0
6	0.0	0.0	0.0	0.7307898979043524	0.0
7	0.0	0.0	0.0	0.8619022031166039	0.0
8	0.0	0.0	0.0	1.2573885008060182	0.0
9	0.0	0.0	0.0	1.4551316496507254	0.0
10	0.0	0.0	0.0	1.6872649113379903	0.0
11	0.0	0.0	0.0	1.9494895217624932	0.0
12	0.0	0.0	0.0	2.170875873186459	0.0
13	0.0	0.0	0.0	2.2568511552928534	0.0
14	0.0	0.0	0.0	2.30843632455669	0.0
15	0.0	0.0	0.0	2.366469639978506	0.0
16	0.0	0.0	0.0	2.4653412144008597	0.0
17	0.0	0.0	0.0	2.6329930145083287	0.0
18	0.0	0.0	0.0	2.830736163353036	0.0
19	0.0	0.0	0.0	2.9210102095647503	0.0
20	0.0	0.0	0.0	3.0392262224610422	0.0
21	0.0	0.0	0.0	3.1660397635679742	0.0
22	0.0	0.0	0.0	3.329392799570124	0.0
23	0.0	0.0	0.0	3.481998925308974	0.0
24	0.0	0.0	0.0	3.6109618484685653	0.0
25	0.0	0.0	0.0	3.703385276732939	0.0
26	0.0	0.0	0.0	3.7893605588393338	0.0
27	0.0	0.0	0.0	3.911875335840946	0.0
28	0.0	0.0	0.0	4.042987641053197	0.0
29	0.0	0.0	0.0	4.15045674368619	0.0
30	0.0	0.0	0.0	4.288017195056422	0.0
31	0.0	0.0	0.0	4.425577646426652	0.0
32	0.0	0.0	0.0	4.558839333691564	0.0
33	0.0	0.0	0.0	4.677055346587856	0.0
34	0.0	0.0	0.0	4.786673831273509	0.0
35	0.0	0.0	0.0	4.941429339065019	0.0
36	0.0	0.0	0.0	5.087587318645889	0.0
37	0.0	0.0	0.0	5.22729715206878	0.0
38	0.0	0.0	0.0	5.343363782912412	0.0
39	0.0	0.0	0.0	5.485222998387964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGTC	35	1.17865056E-7	45.000004	6
AGCCCAC	20	6.963608E-4	45.0	11
ACTAATC	40	6.5756467E-9	45.0	10
GTAGACA	20	6.963608E-4	45.0	42
GGATGAC	20	6.963608E-4	45.0	8
GTCATGA	20	6.963608E-4	45.0	24
TATACTA	40	6.5756467E-9	45.0	44
GGCACCG	25	3.8331687E-5	45.0	8
CCTACAC	40	6.5756467E-9	45.0	28
TGCAAGG	25	3.8331687E-5	45.0	2
TGTCTGG	20	6.963608E-4	45.0	2
TCAGGGT	20	6.963608E-4	45.0	4
GGACTCA	20	6.963608E-4	45.0	8
AAATATA	40	6.5756467E-9	45.0	35
AGAGGGT	20	6.963608E-4	45.0	4
AATTATA	40	6.5756467E-9	45.0	41
TGACAGG	20	6.963608E-4	45.0	2
AGGGTAG	20	6.963608E-4	45.0	6
GAGCCCA	20	6.963608E-4	45.0	10
AAGGGCG	25	3.8331687E-5	45.0	5
>>END_MODULE