FastQCFastQC Report
Thu 26 May 2016
SRR1545349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545349_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2018348
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48760.24158371103496523No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43260.21433370261223533No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT207.0345576E-445.043
ACGTTAG502.1827873E-1145.01
GACCGAA253.8918584E-545.09
CGTTCGA207.0345576E-445.039
GGACGTA207.0345576E-445.08
CTATTCG207.0345576E-445.01
CGGTATG253.8918584E-545.06
CCGTTCG207.0345576E-445.038
TCGAACG253.8918584E-545.02
TACGTGC253.8918584E-545.042
GGTCGTA302.1663218E-644.9999968
TATAGCG950.042.631581
AATACGG1250.041.42
CTATACG556.184564E-1140.9090921
ACGAATG1000.040.51
CGACAGG1750.039.8571432
TATTACG850.039.7058831
GCAAGTT403.4599543E-739.37544
GTTAGCG1550.039.1935461
CACGAAA750.039.00000435
Produced by FastQC (version 0.11.5)