##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545349_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2018348
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.185133584495834	31.0	31.0	33.0	30.0	34.0
2	31.543110008779458	31.0	31.0	34.0	30.0	34.0
3	31.724645601254096	31.0	31.0	34.0	30.0	34.0
4	35.5028924645304	37.0	35.0	37.0	33.0	37.0
5	35.410623936011035	37.0	35.0	37.0	33.0	37.0
6	35.469361576893576	37.0	35.0	37.0	33.0	37.0
7	35.824895904967825	37.0	35.0	37.0	35.0	37.0
8	35.867016986168885	37.0	35.0	37.0	35.0	37.0
9	37.75200114152763	39.0	37.0	39.0	35.0	39.0
10	37.12041233721836	39.0	37.0	39.0	33.0	39.0
11	36.72569447885102	39.0	35.0	39.0	32.0	39.0
12	35.805767389964466	37.0	35.0	39.0	31.0	39.0
13	35.38590768291692	37.0	35.0	39.0	30.0	39.0
14	36.31120797800974	38.0	35.0	41.0	31.0	41.0
15	36.63385947319293	38.0	35.0	41.0	31.0	41.0
16	36.77310305259549	38.0	35.0	41.0	32.0	41.0
17	36.71222702923381	38.0	35.0	41.0	32.0	41.0
18	36.67434654479802	38.0	35.0	40.0	31.0	41.0
19	36.62985421740949	38.0	35.0	40.0	31.0	41.0
20	36.496317285225345	38.0	35.0	40.0	31.0	41.0
21	36.35710145128591	38.0	35.0	40.0	31.0	41.0
22	36.22852897518168	38.0	35.0	40.0	31.0	41.0
23	36.16538426475513	37.0	35.0	40.0	31.0	41.0
24	36.04474897292241	37.0	35.0	40.0	30.0	41.0
25	35.91188437276426	37.0	34.0	40.0	30.0	41.0
26	35.72575591523365	37.0	34.0	40.0	30.0	41.0
27	35.57175769490692	36.0	34.0	40.0	29.0	41.0
28	35.5254985760632	36.0	34.0	40.0	29.0	41.0
29	35.663138864061104	36.0	34.0	40.0	30.0	41.0
30	35.652179901582876	36.0	34.0	40.0	30.0	41.0
31	35.534572828867965	36.0	34.0	40.0	29.0	41.0
32	35.40854203536754	36.0	34.0	40.0	29.0	41.0
33	35.20636332287594	36.0	34.0	40.0	28.0	41.0
34	35.07294579527416	36.0	34.0	40.0	27.0	41.0
35	34.94164039105249	36.0	34.0	40.0	27.0	41.0
36	34.75350137835497	36.0	33.0	40.0	26.0	41.0
37	34.62440025208735	36.0	33.0	40.0	25.0	41.0
38	34.60539262803045	35.0	33.0	40.0	26.0	41.0
39	34.566526684199154	35.0	33.0	40.0	25.0	41.0
40	34.40402745215394	35.0	33.0	40.0	24.0	41.0
41	34.386269860301596	35.0	33.0	40.0	24.0	41.0
42	34.3177464936671	35.0	33.0	40.0	24.0	41.0
43	34.165001278273124	35.0	33.0	40.0	24.0	41.0
44	34.04399043177886	35.0	33.0	40.0	24.0	41.0
45	34.00590334273376	35.0	33.0	40.0	23.0	41.0
46	33.9949230757035	35.0	33.0	39.0	23.0	41.0
47	33.96719544895132	35.0	33.0	39.0	24.0	41.0
48	33.90091500573737	35.0	33.0	39.0	23.0	41.0
49	33.882140245388804	35.0	33.0	39.0	24.0	41.0
50	33.74802759484489	35.0	33.0	39.0	24.0	41.0
51	33.518734133063276	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	7.0
13	13.0
14	29.0
15	85.0
16	212.0
17	545.0
18	1099.0
19	2153.0
20	3708.0
21	5993.0
22	8767.0
23	12584.0
24	17583.0
25	24083.0
26	31357.0
27	37735.0
28	43057.0
29	49934.0
30	59251.0
31	71826.0
32	89195.0
33	116189.0
34	205119.0
35	297562.0
36	145157.0
37	175963.0
38	243652.0
39	375239.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.81500712463856	24.422448457847707	27.51393714067148	13.24860727684225
2	30.351208017646115	25.281368723332147	30.833434075788713	13.53398918323302
3	30.079005206237973	25.604801550575022	30.384799846210864	13.93139339697614
4	26.45510090430391	28.049721851732208	30.10729566952775	15.387881574436124
5	23.84905873516361	33.01140338534287	28.533582910380172	14.605954969113355
6	22.554980607903097	41.36437323989718	25.65385156573594	10.42679458646378
7	88.32679002828056	4.379571808231287	5.421810312195915	1.8718278512922448
8	89.83074276586595	3.118788236716364	5.023960189224058	2.0265088081936318
9	85.22142861389612	4.541833222021178	7.300772711147929	2.9359654529347763
10	54.578150051428196	20.762772326675083	12.083743734975336	12.575333886921383
11	46.35949796566301	20.93851010826676	17.89086916626865	14.81112275980158
12	40.48271160374723	21.154875175143236	23.184158529649	15.178254691460541
13	22.97819801144302	37.31720198895334	23.78058689581777	15.92401310378587
14	18.03242057365727	39.31442942446	26.421063166510432	16.232086835372293
15	16.96793615372572	22.77753885851201	44.16091774064731	16.093607247114967
16	18.790614898917333	17.57115224926524	42.90434553407044	20.733887317746987
17	18.881332654230093	18.621417119347107	27.39274892139512	35.104501305027675
18	23.43525497089699	22.81301341493142	31.545551114079434	22.206180500092152
19	30.29264527227217	23.339384486718842	24.147471100127433	22.220499140881554
20	32.33362135766478	22.198946861492665	23.67748277304013	21.78994900780242
21	25.30440736681682	26.4102127086112	26.21321992044979	22.072160004122182
22	25.218891885839312	24.402927542723056	23.37411586109036	27.004064710347276
23	23.715484148422373	28.90591711637438	22.90080798752247	24.47779074768078
24	23.260161280413485	23.69982777994677	34.22838876150198	18.811622178137764
25	20.78120324146282	24.21911384954428	32.0528967254408	22.946786183552092
26	19.885520237342618	31.06699142070644	25.59444654737439	23.453041794576553
27	21.261199753461742	30.458919869120688	26.871877396762105	21.408002980655468
28	18.554580280506634	27.49540713494402	34.307562422337476	19.64245016221187
29	18.958177677982192	24.017216059866783	33.659953585803834	23.36465267634719
30	21.162207904682443	28.09188504658265	29.51597048675451	21.22993656198039
31	29.123124456238468	25.981594848856588	22.65872882178891	22.236551873116035
32	30.523626252757207	25.452548321696757	24.28803159811886	19.735793827427184
33	27.287117979654646	26.86048193869442	24.29571114594708	21.556688935703853
34	21.071242422020383	26.415761801235465	27.481187585094347	25.03180819164981
35	22.337129176930837	24.588723054696217	29.08353762582072	23.990610142552228
36	31.06758596634475	24.147272918247992	24.383010263839537	20.40213085156772
37	22.878710707965126	29.496053207870993	26.85592375546734	20.769312328696536
38	22.44271057320145	30.979147302645533	22.93593572565286	23.64220639850016
39	22.373545097277574	30.14376113534435	24.9416354365055	22.541058330872573
40	23.96474740728556	25.936409380344717	24.60125805857067	25.497585153799047
41	19.059944073073623	24.40089617845882	25.922338466904616	30.616821281562938
42	23.41330632774923	25.792281608523403	23.253720369331752	27.540691694395612
43	23.177668073097404	26.11259307116513	25.086159572085688	25.623579283651775
44	21.214428829914368	29.059012618240267	26.01300667674752	23.713551875097853
45	20.05992029124809	32.785921952012245	22.264495518116796	24.889662238622872
46	23.36499949463621	30.358392110775746	25.217653249092827	21.058955145495226
47	23.465774980330448	25.88562527373872	26.848293753108976	23.80030599282185
48	24.284513869758833	22.649662000804618	28.93470303436276	24.131121095073794
49	22.03425771967966	23.029576663687333	30.160111140397987	24.77605447623502
50	20.587133636023125	29.80799148610646	26.402186342493962	23.202688535376456
51	20.928155105066125	30.87054363271349	23.516608632406307	24.684692629814087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	593.0
1	1632.0
2	2671.0
3	6349.5
4	10028.0
5	7651.5
6	5275.0
7	5313.5
8	5352.0
9	5669.0
10	5986.0
11	6041.0
12	6096.0
13	6273.5
14	6451.0
15	6134.5
16	5818.0
17	5492.5
18	5167.0
19	5130.5
20	5094.0
21	5128.5
22	5163.0
23	5723.0
24	6283.0
25	7578.0
26	8866.0
27	8859.0
28	12777.0
29	16695.0
30	20443.5
31	24192.0
32	26540.0
33	28888.0
34	34195.5
35	39503.0
36	41339.0
37	43175.0
38	53562.0
39	63949.0
40	86071.5
41	108194.0
42	139344.5
43	170495.0
44	179064.5
45	187634.0
46	180801.0
47	173968.0
48	168147.0
49	162326.0
50	156209.5
51	150093.0
52	140748.0
53	131403.0
54	122925.5
55	114448.0
56	106143.5
57	97839.0
58	94664.5
59	91490.0
60	87350.5
61	83211.0
62	75952.5
63	68694.0
64	60621.5
65	52549.0
66	45115.5
67	37682.0
68	34253.5
69	30825.0
70	25532.5
71	20240.0
72	17782.0
73	15324.0
74	11525.0
75	6508.0
76	5290.0
77	3905.5
78	2521.0
79	1913.0
80	1305.0
81	959.5
82	614.0
83	403.5
84	193.0
85	132.5
86	72.0
87	62.5
88	53.0
89	40.0
90	27.0
91	21.0
92	15.0
93	10.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2018348.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.245619162228195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68112648718784	14.720771074647912
2	7.1897987529267	2.623646597979276
3	2.140163202742085	1.1714580822673997
4	0.9500626542192935	0.6933792547656369
5	0.6212488189093103	0.5667534677401673
6	0.42624280789903696	0.46662383661387724
7	0.33929540089317034	0.4333458267734629
8	0.25191271507891894	0.36770427691622837
9	0.21877407313607403	0.3592501578909239
>10	3.5719114315466953	17.370712521022348
>50	2.4979996939276186	33.838558928764456
>100	1.1059955041587706	26.2051083814455
>500	0.004101343030821069	0.5109497755631683
>1k	0.0013671143436070233	0.6717378176096526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4876	0.24158371103496523	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4326	0.21433370261223533	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.9545469859508865E-5	0.0	0.0	0.06024729134916278	0.0
2	4.9545469859508865E-5	0.0	0.0	0.19020505879065452	0.0
3	4.9545469859508865E-5	0.0	0.0	0.2732432662751914	0.0
4	4.9545469859508865E-5	0.0	0.0	0.4245551312261315	0.0
5	4.9545469859508865E-5	0.0	0.0	0.6951229421289093	0.0
6	4.9545469859508865E-5	0.0	0.0	1.0035930374742117	0.0
7	4.9545469859508865E-5	0.0	0.0	1.1772003638619306	0.0
8	4.9545469859508865E-5	0.0	0.0	1.618600954840295	0.0
9	4.9545469859508865E-5	0.0	0.0	1.7977078283824197	0.0
10	4.9545469859508865E-5	0.0	0.0	2.0726356406328343	0.0
11	4.9545469859508865E-5	0.0	0.0	2.3851189190367568	0.0
12	4.9545469859508865E-5	0.0	0.0	2.633787632261632	0.0
13	4.9545469859508865E-5	0.0	0.0	2.740805847158171	0.0
14	4.9545469859508865E-5	0.0	0.0	2.78326631482777	0.0
15	4.9545469859508865E-5	0.0	0.0	2.8515895177640327	0.0
16	4.9545469859508865E-5	0.0	0.0	3.0071622931228905	0.0
17	4.9545469859508865E-5	0.0	0.0	3.184237802400775	0.0
18	4.9545469859508865E-5	0.0	0.0	3.4101155994902763	0.0
19	4.9545469859508865E-5	0.0	0.0	3.520899270096138	0.0
20	4.9545469859508865E-5	0.0	0.0	3.6339620323155373	0.0
21	4.9545469859508865E-5	0.0	0.0	3.795034354828801	0.0
22	4.9545469859508865E-5	0.0	0.0	3.953877131198386	0.0
23	4.9545469859508865E-5	0.0	0.0	4.142645371363114	0.0
24	4.9545469859508865E-5	0.0	0.0	4.294155418193493	0.0
25	4.9545469859508865E-5	0.0	0.0	4.415145455590413	0.0
26	4.9545469859508865E-5	0.0	0.0	4.529595490965879	0.0
27	4.9545469859508865E-5	0.0	0.0	4.642063707546964	0.0
28	4.9545469859508865E-5	0.0	0.0	4.7648869273286865	0.0
29	4.9545469859508865E-5	0.0	0.0	4.892367421277203	0.0
30	4.9545469859508865E-5	0.0	0.0	5.051160652176929	0.0
31	4.9545469859508865E-5	0.0	0.0	5.205891154548175	0.0
32	4.9545469859508865E-5	0.0	0.0	5.345064379383535	0.0
33	4.9545469859508865E-5	0.0	0.0	5.482701694653251	0.0
34	4.9545469859508865E-5	0.0	0.0	5.630743558593464	0.0
35	4.9545469859508865E-5	0.0	0.0	5.815102251940696	0.0
36	4.9545469859508865E-5	0.0	0.0	5.968841844914752	0.0
37	4.9545469859508865E-5	0.0	0.0	6.124761438562627	0.0
38	4.9545469859508865E-5	0.0	0.0	6.276420121802583	0.0
39	4.9545469859508865E-5	0.0	0.0	6.433826079546243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.0345576E-4	45.0	43
ACGTTAG	50	2.1827873E-11	45.0	1
GACCGAA	25	3.8918584E-5	45.0	9
CGTTCGA	20	7.0345576E-4	45.0	39
GGACGTA	20	7.0345576E-4	45.0	8
CTATTCG	20	7.0345576E-4	45.0	1
CGGTATG	25	3.8918584E-5	45.0	6
CCGTTCG	20	7.0345576E-4	45.0	38
TCGAACG	25	3.8918584E-5	45.0	2
TACGTGC	25	3.8918584E-5	45.0	42
GGTCGTA	30	2.1663218E-6	44.999996	8
TATAGCG	95	0.0	42.63158	1
AATACGG	125	0.0	41.4	2
CTATACG	55	6.184564E-11	40.909092	1
ACGAATG	100	0.0	40.5	1
CGACAGG	175	0.0	39.857143	2
TATTACG	85	0.0	39.705883	1
GCAAGTT	40	3.4599543E-7	39.375	44
GTTAGCG	155	0.0	39.193546	1
CACGAAA	75	0.0	39.000004	35
>>END_MODULE