FastQCFastQC Report
Thu 26 May 2016
SRR1545348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545348_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3361789
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94110.27994023420268194No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC40470.12038233214517627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT551.8189894E-1245.028
CCGTCGA253.8924147E-545.011
GTCGTCG207.035227E-445.045
TCGACGC253.8924147E-545.037
ACGCGGT253.8924147E-545.03
CGTTTTT104850.043.068671
TCGAGCG700.041.7857131
CGTAAGG6700.040.6343272
GCGCGAG1850.040.1351361
TATTGCG2300.040.1086921
AACGGGA16450.039.939214
GCGTAAG3750.039.01
ACGGGAT25100.038.814745
CGTTAGG5750.038.7391322
TACGGGA19050.038.6220444
CGGTACG356.251881E-638.5714262
AGGGCGA38750.038.264526
ACGGGAC14350.038.1010445
GGGCGAT75900.037.76687
AAGGGAT75150.037.724555
Produced by FastQC (version 0.11.5)