FastQCFastQC Report
Thu 26 May 2016
SRR1545347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545347_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2798779
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102850.3674816768312182No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37730.1348087862600084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC1900.043.815799
CGTTTTT78300.039.9712641
CATACGA3350.039.62686518
TCGATAG1450.038.7931061
TACGGGA5600.038.571434
CGTAAGG4800.038.4375042
TAACGAC301.1403405E-437.50000419
GGCGATA16150.037.198148
CTATACG552.752131E-936.818181
ACACGAC7850.036.687926
GGGCGAT70550.036.420987
AACGTAG1500.036.0000041
CGCAACT250.00210762436.033
CACGACC8150.035.88957227
TATGGGC13500.035.6666684
CGATACG950.035.52631410
ATACTAT13050.035.51724245
ACGACCA8250.035.45454828
GCGATAT4700.035.4255339
ACATACG3750.035.39999817
Produced by FastQC (version 0.11.5)