##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545347_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2798779
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27324272477391	31.0	31.0	33.0	30.0	34.0
2	31.642063914299772	31.0	31.0	34.0	30.0	34.0
3	31.807308830029093	31.0	31.0	34.0	30.0	34.0
4	35.606001760053225	37.0	35.0	37.0	33.0	37.0
5	35.54235329048846	37.0	35.0	37.0	33.0	37.0
6	35.59995590934475	37.0	35.0	37.0	33.0	37.0
7	35.88917095633489	37.0	35.0	37.0	35.0	37.0
8	35.9226044643039	37.0	35.0	37.0	35.0	37.0
9	37.82874281963671	39.0	38.0	39.0	35.0	39.0
10	37.23609331069012	39.0	37.0	39.0	34.0	39.0
11	36.89151233448586	39.0	37.0	39.0	33.0	39.0
12	36.256615474104954	38.0	35.0	39.0	32.0	39.0
13	35.996899362186156	38.0	35.0	39.0	31.0	39.0
14	36.95331178345986	39.0	35.0	41.0	31.0	41.0
15	37.169387436449966	39.0	35.0	41.0	32.0	41.0
16	37.23637772042737	39.0	35.0	41.0	32.0	41.0
17	37.15330327975163	39.0	35.0	41.0	32.0	41.0
18	37.086696377241644	39.0	35.0	41.0	32.0	41.0
19	37.032855041430565	38.0	35.0	41.0	32.0	41.0
20	36.92179160984129	38.0	35.0	41.0	32.0	41.0
21	36.8110590368157	38.0	35.0	41.0	31.0	41.0
22	36.72376525620637	38.0	35.0	41.0	31.0	41.0
23	36.626936960724656	38.0	35.0	40.0	31.0	41.0
24	36.525991512727515	38.0	35.0	40.0	31.0	41.0
25	36.39110697915055	38.0	35.0	40.0	31.0	41.0
26	36.22939324612626	38.0	35.0	40.0	30.0	41.0
27	36.10999189289329	38.0	35.0	40.0	30.0	41.0
28	35.99453511692063	38.0	35.0	40.0	30.0	41.0
29	36.115887678162515	38.0	35.0	40.0	30.0	41.0
30	36.09488459074475	38.0	35.0	40.0	30.0	41.0
31	35.97107631577913	38.0	35.0	40.0	30.0	41.0
32	35.83665805695984	38.0	34.0	40.0	30.0	41.0
33	35.640009804275365	38.0	34.0	40.0	29.0	41.0
34	35.43094792407689	38.0	34.0	40.0	28.0	41.0
35	35.29602051466014	37.0	34.0	40.0	27.0	41.0
36	35.15963997157332	37.0	34.0	40.0	27.0	41.0
37	35.06250582843447	37.0	34.0	40.0	27.0	41.0
38	34.974683960398444	37.0	34.0	40.0	27.0	41.0
39	34.89272286236248	37.0	34.0	40.0	26.0	41.0
40	34.74395906214817	37.0	34.0	40.0	25.0	41.0
41	34.6640685098752	36.0	34.0	40.0	25.0	41.0
42	34.59777674478764	36.0	33.0	40.0	25.0	41.0
43	34.473979903379295	36.0	33.0	40.0	25.0	41.0
44	34.34396856629266	36.0	33.0	40.0	24.0	41.0
45	34.311356487954214	35.0	33.0	40.0	24.0	41.0
46	34.32039828796772	35.0	33.0	40.0	24.0	41.0
47	34.28445547147524	35.0	33.0	40.0	24.0	41.0
48	34.19106617564302	35.0	33.0	40.0	24.0	41.0
49	34.13841035680202	35.0	33.0	40.0	24.0	41.0
50	34.021276778195066	35.0	33.0	39.0	24.0	41.0
51	33.78174589705011	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	7.0
13	11.0
14	35.0
15	91.0
16	264.0
17	593.0
18	1264.0
19	2567.0
20	4343.0
21	6801.0
22	10287.0
23	14929.0
24	21457.0
25	30364.0
26	40520.0
27	48040.0
28	54174.0
29	62454.0
30	74705.0
31	91068.0
32	114149.0
33	150923.0
34	255067.0
35	338219.0
36	224675.0
37	284281.0
38	391418.0
39	575821.0
40	244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.3268693240874	23.116008802409908	27.733165069482084	13.82395680402061
2	32.5250403836816	25.014336608928396	29.085862084859148	13.374760922530859
3	30.18798554655441	25.812791935340375	30.106342801628855	13.892879716476363
4	26.73440811153721	27.867080609079885	30.04084995635597	15.357661323026933
5	23.90828286191943	32.08877871386058	29.00639886178937	14.996539562430616
6	22.771572889463584	40.81511973614208	25.624209700015616	10.78909767437872
7	88.25498547759577	3.928570280111434	6.012836311834554	1.8036079304582464
8	89.23276900391205	2.9764050680671823	5.838010075107753	1.9528158529130024
9	84.75202936709186	4.358221924632135	7.646048509010536	3.2437001992654655
10	49.474645908090636	22.788294466980066	13.911423517183744	13.825636107745556
11	39.94295369516493	23.512253021764135	20.11298498380901	16.431808299261927
12	34.358697131856424	22.422420634140817	25.70963981078892	17.509242423213838
13	24.634742507357675	29.88224507901481	27.121469755203965	18.36154265842355
14	20.855344419834505	31.490517829382025	29.428511504481058	18.225626246302408
15	20.42008318627516	22.530289101068714	39.00379415452238	18.045833558133744
16	23.53429834938736	20.281022545903053	36.42838537805236	19.75629372665723
17	23.487920982685665	20.311250012952076	27.528718773436562	28.6721102309257
18	26.340593523104182	22.539614596222137	29.823183609709808	21.296608270963873
19	30.047781550454683	23.97588376931512	24.7470772075966	21.229257472633602
20	31.341095527728342	24.091648536736912	23.914714237887306	20.65254169764744
21	27.312160052651528	25.269519315387175	26.436349565292577	20.981971066668713
22	26.442959590592896	23.30512698573199	25.553464564368962	24.698448859306147
23	24.575823957518615	26.64154618853436	25.459280636306048	23.323349217640978
24	24.627775183392473	23.670536330306895	31.114460984593638	20.587227501706995
25	23.473736225689844	23.675359862282804	29.23056804413639	23.62033586789096
26	22.01084830206315	27.806589945115352	26.46454042995178	23.718021322869724
27	22.419597974688248	26.33562707166232	27.91306494725021	23.33171000639922
28	21.111777671620374	25.931200712882298	31.305401391106624	21.651620224390708
29	22.49970433535481	24.54330977901435	30.547892491690128	22.409093393940715
30	24.14031261489385	25.22389227588173	29.14049305071962	21.495302058504798
31	28.211766631091628	24.5955825736866	25.22657201586835	21.966078779353428
32	29.42972631994166	24.68901617455326	25.042849042385985	20.838408463119094
33	27.96512336272353	24.705130344339445	25.130351485415602	22.199394807521422
34	23.15788420593409	25.432018748175544	28.858655863860633	22.551441182029734
35	23.769579520212204	25.77820542457979	28.415676979139832	22.036538076068172
36	28.698514602260484	26.239299351610114	24.79845675560664	20.26372929052276
37	25.16254409512148	28.46952188793756	25.40039781633348	20.96753620060748
38	24.355227761820423	28.682650541539722	24.35308396983113	22.609037726808726
39	24.997936600210306	27.70647485921539	24.560102816263807	22.735485724310493
40	25.593446284969268	24.496503653914797	25.316361170353215	24.59368889076272
41	21.909482670836102	24.06556573420052	25.94552838934407	28.079423205619307
42	23.86640745839525	25.265517570340496	24.877526950145047	25.99054802111921
43	23.871695478635505	25.17880118437362	26.05914936477657	24.890353972214314
44	23.41103031000304	26.754702675702514	26.95035942459194	22.88390758970251
45	21.963899257497644	28.19415180691294	25.10437587247868	24.737573063110734
46	23.343929620738184	27.09206407508417	26.463289884624686	23.100716419552956
47	23.593967226422667	25.429267548455954	27.7585690045552	23.21819622056618
48	24.704415747009676	23.590001211242473	28.187720430945067	23.51786261080278
49	23.273184485091537	23.327422422420636	29.169255593242628	24.23013749924521
50	21.653871205979463	27.90645492194989	26.617249879322376	23.822423992748266
51	21.976083141970122	28.463876569032426	25.398754242475025	24.161286046522427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	791.0
1	2148.5
2	3506.0
3	11884.0
4	20262.0
5	14652.5
6	9043.0
7	8981.0
8	8919.0
9	9189.0
10	9459.0
11	9509.5
12	9560.0
13	9393.0
14	9226.0
15	9110.0
16	8994.0
17	8986.5
18	8979.0
19	8585.5
20	8192.0
21	9174.5
22	10157.0
23	10343.0
24	10529.0
25	11877.5
26	16286.5
27	19347.0
28	20532.5
29	21718.0
30	27134.5
31	32551.0
32	38111.5
33	43672.0
34	51502.0
35	59332.0
36	64003.0
37	68674.0
38	79355.5
39	90037.0
40	111680.0
41	133323.0
42	154595.0
43	175867.0
44	183036.0
45	190205.0
46	195479.0
47	200753.0
48	197605.5
49	194458.0
50	199015.0
51	203572.0
52	195575.0
53	187578.0
54	177826.5
55	168075.0
56	164265.5
57	160456.0
58	156415.5
59	152375.0
60	147256.5
61	142138.0
62	131610.0
63	121082.0
64	105632.0
65	90182.0
66	79317.0
67	68452.0
68	60547.0
69	52642.0
70	43326.5
71	34011.0
72	28823.0
73	23635.0
74	19465.0
75	11984.0
76	8673.0
77	6868.0
78	5063.0
79	3906.5
80	2750.0
81	1901.5
82	1053.0
83	817.0
84	581.0
85	402.0
86	223.0
87	152.5
88	82.0
89	66.5
90	51.0
91	34.0
92	17.0
93	12.0
94	7.0
95	5.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2798779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.975425936787207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51232319184479	14.898047738519512
2	6.982027185198304	2.649738794827306
3	2.280484147841165	1.2981947414213195
4	1.189280527192027	0.9026841824718221
5	0.7604478077696171	0.7214910527562282
6	0.6206798102418107	0.706659826182157
7	0.44521541715877844	0.5913706521948554
8	0.3767378507904507	0.571900894820687
9	0.3179640300362555	0.5430152612263814
>10	5.198090148216421	25.858531144648616
>50	2.7684670845270953	37.25775097061293
>100	0.5452389300343695	13.117736703793046
>500	0.0019024182180860949	0.21627028322454153
>1k	9.512091090430474E-4	0.29532752246035293
>5k	0.0	0.0
>10k+	1.902418218086095E-4	0.3712802308403288
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10285	0.3674816768312182	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3773	0.1348087862600084	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026690210266691296	0.0
2	0.0	0.0	0.0	0.08882444808968483	0.0
3	0.0	0.0	0.0	0.14856478485796842	0.0
4	0.0	0.0	0.0	0.2421770350570731	0.0
5	0.0	0.0	0.0	0.4169318120508979	0.0
6	0.0	0.0	0.0	0.7367141171203585	0.0
7	0.0	0.0	0.0	0.920008332204865	0.0
8	0.0	0.0	0.0	1.3474089951368078	0.0
9	0.0	0.0	0.0	1.5972322216223576	0.0
10	0.0	0.0	0.0	1.9022223619657	0.0
11	0.0	0.0	0.0	2.190240815727144	0.0
12	0.0	0.0	0.0	2.416053571932618	0.0
13	0.0	0.0	0.0	2.534533809207515	0.0
14	0.0	0.0	0.0	2.5966323171640204	0.0
15	0.0	0.0	0.0	2.6617321339055353	0.0
16	0.0	0.0	0.0	2.7781757687906046	0.0
17	0.0	0.0	0.0	2.904230737761002	0.0
18	0.0	0.0	0.0	3.0681593652089	3.2156879839387103E-4
19	0.0	0.0	0.0	3.162843511402651	3.2156879839387103E-4
20	0.0	0.0	0.0	3.2660670956870836	3.2156879839387103E-4
21	0.0	0.0	0.0	3.391907685458552	3.2156879839387103E-4
22	0.0	0.0	0.0	3.531647193293933	3.2156879839387103E-4
23	0.0	0.0	0.0	3.688251198111748	3.2156879839387103E-4
24	0.0	0.0	0.0	3.8148063852129805	3.2156879839387103E-4
25	0.0	0.0	0.0	3.9210670081489107	3.2156879839387103E-4
26	0.0	0.0	0.0	4.0297215321395505	3.2156879839387103E-4
27	0.0	0.0	0.0	4.142842289441217	3.2156879839387103E-4
28	0.0	0.0	0.0	4.256999212871041	3.2156879839387103E-4
29	0.0	0.0	0.0	4.376944374671955	3.2156879839387103E-4
30	0.0	0.0	0.0	4.541909168248011	3.2156879839387103E-4
31	3.5729866488207895E-5	0.0	0.0	4.678647367298383	3.2156879839387103E-4
32	3.5729866488207895E-5	0.0	0.0	4.824675331635688	3.2156879839387103E-4
33	3.5729866488207895E-5	0.0	0.0	4.966773010659291	3.2156879839387103E-4
34	3.5729866488207895E-5	0.0	0.0	5.113694221658802	3.2156879839387103E-4
35	3.5729866488207895E-5	0.0	0.0	5.286912614393634	3.2156879839387103E-4
36	3.5729866488207895E-5	0.0	0.0	5.441265637622692	3.2156879839387103E-4
37	3.5729866488207895E-5	0.0	0.0	5.596333258181514	3.2156879839387103E-4
38	3.5729866488207895E-5	0.0	0.0	5.7881669113567025	3.2156879839387103E-4
39	3.5729866488207895E-5	0.0	0.0	6.06971825928378	3.2156879839387103E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAC	190	0.0	43.81579	9
CGTTTTT	7830	0.0	39.971264	1
CATACGA	335	0.0	39.626865	18
TCGATAG	145	0.0	38.793106	1
TACGGGA	560	0.0	38.57143	4
CGTAAGG	480	0.0	38.437504	2
TAACGAC	30	1.1403405E-4	37.500004	19
GGCGATA	1615	0.0	37.19814	8
CTATACG	55	2.752131E-9	36.81818	1
ACACGAC	785	0.0	36.6879	26
GGGCGAT	7055	0.0	36.42098	7
AACGTAG	150	0.0	36.000004	1
CGCAACT	25	0.002107624	36.0	33
CACGACC	815	0.0	35.889572	27
TATGGGC	1350	0.0	35.666668	4
CGATACG	95	0.0	35.526314	10
ATACTAT	1305	0.0	35.517242	45
ACGACCA	825	0.0	35.454548	28
GCGATAT	470	0.0	35.425533	9
ACATACG	375	0.0	35.399998	17
>>END_MODULE