FastQCFastQC Report
Thu 26 May 2016
SRR1545346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545346_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2921859
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70200.2402580001293697No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC60000.2053487180592903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA351.212793E-745.00000431
CTATCGA453.8562575E-1045.014
TAGTTCG253.8922888E-545.01
TCGCTAA253.8922888E-545.037
TACGACG603.6379788E-1241.2500041
CTATACG1100.040.9090921
TCGTACA501.0822987E-940.541
CTATGCG451.9301297E-840.01
CGTTTTT64800.039.7916681
CGTAAGG4500.039.52
ACACGTC403.4606637E-739.37512
TACGGGT2550.038.823534
TACGGGA11050.038.6877864
AGGGCGA37800.038.6309556
CGACGAA356.2515974E-638.571432
CCTAACG356.2515974E-638.5714342
ATCGGCA356.2515974E-638.5714339
GGGCGAT69750.038.1290327
CTACGGG6050.037.9338873
TAACACG950.037.8947371