FastQCFastQC Report
Thu 26 May 2016
SRR1545345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545345_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4171849
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101940.24435208465119423No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC77900.18672775548683568No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC207.035422E-445.00000411
ACCGTAC253.892577E-545.041
CGTTTTT121600.042.5205571
CGTAAGG6850.040.4014632
GTTAGCG2900.039.568971
ACGGGTA7250.039.4137955
GCGATCG800.039.3750049
TCGAACG750.039.01
CGACGGT2300.038.15217228
TACGGGA10950.037.808224
AGGGCGA47350.037.634646
GCGTAAG3250.037.3846171
TACGGGT5600.037.366074
CGTTGAT5450.037.15596425
TAGGGCG20500.037.0975575
GGCGATA18750.037.0799988
TAGGGTA30550.036.8985255
CGGGTAT6850.036.7883226
ACGTAGG7750.036.5806472
CTTGCGT1050.036.42857415
Produced by FastQC (version 0.11.5)