##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545345_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4171849
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.261050435909834	31.0	31.0	33.0	30.0	34.0
2	31.629372012266025	31.0	31.0	34.0	30.0	34.0
3	31.793481739152114	31.0	31.0	34.0	30.0	34.0
4	35.58536730356252	37.0	35.0	37.0	33.0	37.0
5	35.51307298034996	37.0	35.0	37.0	33.0	37.0
6	35.581007605979984	37.0	35.0	37.0	33.0	37.0
7	35.93886787369342	37.0	35.0	37.0	35.0	37.0
8	35.994777855094945	37.0	35.0	37.0	35.0	37.0
9	37.909447106067354	39.0	38.0	39.0	35.0	39.0
10	37.20085913943674	39.0	37.0	39.0	33.0	39.0
11	36.88761841571927	39.0	37.0	39.0	33.0	39.0
12	36.24958837196648	38.0	35.0	39.0	32.0	39.0
13	35.99458825091704	38.0	35.0	39.0	32.0	39.0
14	37.00236154280752	39.0	35.0	41.0	32.0	41.0
15	37.231440543509606	39.0	35.0	41.0	32.0	41.0
16	37.30746007345903	39.0	35.0	41.0	32.0	41.0
17	37.24871993209725	39.0	35.0	41.0	32.0	41.0
18	37.183385832037544	39.0	35.0	41.0	32.0	41.0
19	37.1579774339867	38.0	35.0	41.0	32.0	41.0
20	37.02756451635714	38.0	35.0	41.0	32.0	41.0
21	36.926302701751666	38.0	35.0	41.0	32.0	41.0
22	36.83127457393592	38.0	35.0	41.0	31.0	41.0
23	36.75571143634393	38.0	35.0	41.0	31.0	41.0
24	36.66859083346497	38.0	35.0	40.0	31.0	41.0
25	36.58192278771355	38.0	35.0	40.0	31.0	41.0
26	36.4659241022386	38.0	35.0	40.0	31.0	41.0
27	36.34794955426239	38.0	35.0	40.0	31.0	41.0
28	36.29846334323222	38.0	35.0	40.0	31.0	41.0
29	36.415950097906226	38.0	35.0	40.0	31.0	41.0
30	36.38417689614365	38.0	35.0	40.0	31.0	41.0
31	36.2784328963009	38.0	35.0	40.0	30.0	41.0
32	36.132734190523195	38.0	35.0	40.0	30.0	41.0
33	35.94013661568288	38.0	35.0	40.0	30.0	41.0
34	35.78174138133954	38.0	35.0	40.0	29.0	41.0
35	35.63845719248228	38.0	34.0	40.0	29.0	41.0
36	35.49972973614337	38.0	34.0	40.0	28.0	41.0
37	35.38977465387649	38.0	34.0	40.0	27.0	41.0
38	35.355693123121185	38.0	34.0	40.0	27.0	41.0
39	35.326293688961414	38.0	34.0	40.0	27.0	41.0
40	35.21187511820298	38.0	34.0	40.0	27.0	41.0
41	35.16700964009004	37.0	34.0	40.0	27.0	41.0
42	35.110295219218145	37.0	34.0	40.0	27.0	41.0
43	35.00943202881984	37.0	34.0	40.0	27.0	41.0
44	34.9048774296481	37.0	34.0	40.0	26.0	41.0
45	34.86964125499269	37.0	34.0	40.0	26.0	41.0
46	34.889162095751786	37.0	34.0	40.0	26.0	41.0
47	34.86519071040203	37.0	34.0	40.0	26.0	41.0
48	34.793790714860485	37.0	34.0	40.0	26.0	41.0
49	34.770895111496124	36.0	34.0	40.0	26.0	41.0
50	34.65213889572705	36.0	34.0	40.0	26.0	41.0
51	34.41877043008987	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	2.0
12	3.0
13	7.0
14	23.0
15	82.0
16	211.0
17	573.0
18	1336.0
19	2822.0
20	4973.0
21	8109.0
22	12776.0
23	19210.0
24	29027.0
25	42704.0
26	56145.0
27	65609.0
28	71796.0
29	83399.0
30	101158.0
31	127635.0
32	162148.0
33	214074.0
34	362782.0
35	526099.0
36	310541.0
37	394011.0
38	578745.0
39	995406.0
40	437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.47527678973999	23.561615005720483	29.483905098195066	13.479203106344453
2	30.204808467420563	25.770012289514792	30.672706514545467	13.352472728519176
3	28.74917093116266	25.304726992755487	31.728137811315797	14.217964264766056
4	25.158916346205245	28.015419541790703	31.66703780505958	15.158626306944475
5	23.11044814901019	32.86118457307539	30.30215139617949	13.726215881734932
6	20.6307802607429	42.17621491094236	26.51663566922005	10.676369159094683
7	89.47739958948658	3.5397254310978177	5.639801440560289	1.3430735388553132
8	91.34896780779937	2.194135022624261	4.902814075964878	1.5540830936114898
9	86.45770736189158	4.221916948576039	6.985128176978601	2.3352475125537864
10	45.639978819942904	23.54816773090301	17.01720268398976	13.794650765164318
11	38.90984549057265	24.772061500787782	20.315596273978276	16.0024967346613
12	33.3385748141891	22.518768057041374	27.416668244703967	16.725988884065554
13	21.83451510349488	34.98352888611261	27.362184010015707	15.819772000376812
14	18.72016460806707	36.75224103269318	28.689868688919468	15.837725670320282
15	17.39382225962637	25.37630197066097	41.40504605991253	15.82482970980014
16	18.8247465332518	21.352115093331516	40.35388145639979	19.469256917016892
17	19.508639933995696	22.369218061344025	28.574644000777592	29.547498003882687
18	22.423582445098084	24.777838315816318	32.006024187356736	20.79255505172886
19	27.530718393690663	26.332328902604097	25.564012503808264	20.572940199896976
20	29.02626629103786	24.865425378531196	25.781733710879756	20.326574619551188
21	23.349838404985416	27.92251109759725	27.559602468833365	21.168048028583968
22	23.763012515553655	25.997393481882973	25.93286573890857	24.306728263654797
23	20.707892351808514	31.01212436020575	25.22982015887919	23.050163129106544
24	21.335216111609025	26.54652649221005	33.73554507845322	18.382712317727705
25	19.396866952758838	27.466118740155743	31.722768489463544	21.414245817621875
26	19.450368409786645	31.63748256468535	27.694698441865945	21.217450583662064
27	20.08212665415263	30.68279796320528	27.880731061934412	21.35434432070768
28	18.431755320003194	28.681598974459526	33.53932512897758	19.3473205765597
29	18.764869006524446	25.26992228146321	33.835500757577755	22.129707954434593
30	20.491801117442172	28.782417580310316	30.204904348167922	20.520876954079593
31	25.798369020547003	27.432896061194928	25.63601894507687	21.132715973181195
32	26.31346436556069	27.499988614161254	26.142508993014847	20.044038027263213
33	25.4367547818725	27.87260516859551	25.630505802103578	21.06013424742842
34	20.829780751892027	27.427550709529513	28.644013721493756	23.0986548170847
35	21.1214499853662	27.0594645204081	29.454086185765593	22.36499930846011
36	28.215211049105566	25.6918455102282	25.898468520792576	20.19447491987366
37	21.64114760625325	30.24227386945213	27.58513071781841	20.531447806476216
38	21.895998632740543	30.845699352972744	25.062004880809447	22.196297133477266
39	21.663080327212224	29.87536221948589	26.322884648988975	22.138672804312908
40	23.493923198083152	26.751807172311366	25.32148215335694	24.432787476248542
41	19.849663782174282	25.558091867658682	27.121187751522168	27.47105659864487
42	21.854793881561868	28.11094073634976	24.898048802821005	25.136216579267373
43	22.18958548116195	27.969996037728116	26.516731549967414	23.32368693114252
44	21.258295782038132	29.84865943134567	26.964710371827938	21.928334414788264
45	19.57585233789622	32.17789042700251	24.571431036933504	23.674826198167768
46	22.42140115809561	30.210129848899133	26.65599833551023	20.712470657495032
47	22.19586567011414	27.45763329401424	27.293149871915308	23.053351163956318
48	23.173705472082045	25.122026228657845	28.600819444807325	23.103448854452786
49	21.36910995580137	24.939109732878634	30.083782994063302	23.607997317256686
50	20.55170261435637	30.161182727371006	26.924248696441317	22.362865961831314
51	19.929868027342312	30.97233385005066	25.244322121917644	23.853476000689383
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	722.0
1	1686.5
2	2651.0
3	11456.0
4	20261.0
5	15745.0
6	11229.0
7	11671.0
8	12113.0
9	13279.0
10	14445.0
11	14450.0
12	14455.0
13	14346.0
14	14237.0
15	13798.5
16	13360.0
17	13509.5
18	13659.0
19	13581.0
20	13503.0
21	14590.0
22	15677.0
23	16477.5
24	17278.0
25	22146.5
26	29410.0
27	31805.0
28	37363.0
29	42921.0
30	54677.0
31	66433.0
32	78758.0
33	91083.0
34	108597.5
35	126112.0
36	137666.5
37	149221.0
38	169821.0
39	190421.0
40	232282.5
41	274144.0
42	314569.5
43	354995.0
44	357258.0
45	359521.0
46	354151.5
47	348782.0
48	345968.5
49	343155.0
50	344380.0
51	345605.0
52	326158.0
53	306711.0
54	267438.0
55	228165.0
56	205789.0
57	183413.0
58	165698.0
59	147983.0
60	133363.0
61	118743.0
62	103659.5
63	88576.0
64	77752.0
65	66928.0
66	54253.5
67	41579.0
68	33471.5
69	25364.0
70	22304.0
71	19244.0
72	15950.5
73	12657.0
74	10167.5
75	6147.0
76	4616.0
77	3536.5
78	2457.0
79	2044.5
80	1632.0
81	1138.5
82	645.0
83	491.5
84	338.0
85	254.0
86	170.0
87	145.5
88	121.0
89	69.0
90	17.0
91	12.0
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4171849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.932123168075243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3086869213699	14.042410190042478
2	8.614311481384968	3.0894578898471985
3	2.8335897063525617	1.5243683886631287
4	1.3878343267955704	0.9954726433992382
5	0.8614098911116169	0.7723454132805898
6	0.5854947556608382	0.6299498443663363
7	0.42603054366964177	0.5347742527712253
8	0.3403797258047975	0.48829849336378467
9	0.28089874655653385	0.4533399828908745
>10	3.3556884887988603	14.234926138150323
>50	1.4367715293101215	18.88599253785169
>100	1.5625619865014553	43.14483866127402
>500	0.005397358879237	0.6133093458196551
>1k	6.74669859904625E-4	0.155365893774727
>5k	1.34933971980925E-4	0.18849093793881377
>10k+	1.34933971980925E-4	0.246659386565888
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10194	0.24435208465119423	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7790	0.18672775548683568	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03935904679196203	0.0
2	0.0	0.0	0.0	0.11846066336533273	0.0
3	0.0	0.0	0.0	0.18878919155511142	0.0
4	0.0	0.0	0.0	0.3274327522400739	0.0
5	0.0	0.0	0.0	0.5608784018788792	0.0
6	0.0	0.0	0.0	0.9527190461591492	0.0
7	0.0	0.0	0.0	1.1629855251232726	0.0
8	0.0	0.0	0.0	1.7125979391871566	0.0
9	0.0	0.0	0.0	1.9804168367551174	0.0
10	0.0	0.0	0.0	2.3493899227896313	0.0
11	0.0	0.0	0.0	2.7050595551277143	0.0
12	0.0	0.0	0.0	3.0069400881959054	0.0
13	0.0	0.0	0.0	3.1492031470937705	0.0
14	0.0	0.0	0.0	3.2122207682972226	0.0
15	0.0	0.0	0.0	3.2843710306868727	0.0
16	0.0	0.0	0.0	3.439146527115435	0.0
17	0.0	0.0	0.0	3.6312915448282044	0.0
18	0.0	0.0	0.0	3.8735582232242827	0.0
19	0.0	0.0	0.0	4.013759846053872	0.0
20	0.0	0.0	0.0	4.168559312669275	0.0
21	0.0	0.0	0.0	4.3699088821287635	0.0
22	0.0	0.0	0.0	4.581877244358557	0.0
23	0.0	0.0	0.0	4.825797865646623	0.0
24	0.0	0.0	0.0	5.007851434699578	0.0
25	0.0	0.0	0.0	5.162483110007098	0.0
26	0.0	0.0	0.0	5.310594894494024	0.0
27	0.0	0.0	0.0	5.4608160554229075	0.0
28	0.0	0.0	0.0	5.631052322363537	0.0
29	0.0	0.0	0.0	5.816102164771544	0.0
30	0.0	0.0	0.0	6.038473588090077	0.0
31	0.0	0.0	0.0	6.237186436997121	0.0
32	0.0	0.0	0.0	6.427317959015295	0.0
33	0.0	0.0	0.0	6.618384318320246	0.0
34	0.0	0.0	0.0	6.822346638145341	0.0
35	0.0	0.0	0.0	7.056151840586752	0.0
36	0.0	0.0	0.0	7.270061787950619	0.0
37	0.0	0.0	0.0	7.495021991447917	0.0
38	0.0	0.0	0.0	7.716266815984951	0.0
39	0.0	0.0	0.0	7.942281707703227	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTATC	20	7.035422E-4	45.000004	11
ACCGTAC	25	3.892577E-5	45.0	41
CGTTTTT	12160	0.0	42.520557	1
CGTAAGG	685	0.0	40.401463	2
GTTAGCG	290	0.0	39.56897	1
ACGGGTA	725	0.0	39.413795	5
GCGATCG	80	0.0	39.375004	9
TCGAACG	75	0.0	39.0	1
CGACGGT	230	0.0	38.152172	28
TACGGGA	1095	0.0	37.80822	4
AGGGCGA	4735	0.0	37.63464	6
GCGTAAG	325	0.0	37.384617	1
TACGGGT	560	0.0	37.36607	4
CGTTGAT	545	0.0	37.155964	25
TAGGGCG	2050	0.0	37.097557	5
GGCGATA	1875	0.0	37.079998	8
TAGGGTA	3055	0.0	36.898525	5
CGGGTAT	685	0.0	36.788322	6
ACGTAGG	775	0.0	36.580647	2
CTTGCGT	105	0.0	36.428574	15
>>END_MODULE