FastQCFastQC Report
Thu 26 May 2016
SRR1545343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545343_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1327221
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.4084474251085539No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16880.127183038845829No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG406.8157533E-945.0000041
ATTAGCG650.041.538461
TATAGCG1050.040.7142831
TACGGGA3050.039.8360674
TTACGGG4100.039.5121963
CGTTAGG1950.039.230772
CTATGCG750.039.0000041
CGTTTTT42300.038.5638281
GGGCGAT30000.038.0249987
GCGATAT1550.037.741949
ATAGGGC6700.037.6119424
ATAGCGG2400.037.4999962
CATAGCG601.5643309E-1037.4999961
TCGTAAG301.14001734E-437.4999961
CCGACAA301.14001734E-437.49999632
GCGTTAG850.037.0588261
AGGGATC7750.036.8709686
TATACGG1600.036.5625042
ACGGGTA1050.036.428575
AGGGCGA14950.036.4214066
Produced by FastQC (version 0.11.5)