##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545343_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1327221
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217788145305114	31.0	31.0	33.0	30.0	33.0
2	31.592196024625892	31.0	31.0	34.0	30.0	34.0
3	31.75474544179153	31.0	31.0	34.0	30.0	34.0
4	35.5660888427775	37.0	35.0	37.0	33.0	37.0
5	35.49431556613405	37.0	35.0	37.0	33.0	37.0
6	35.54678233692806	37.0	35.0	37.0	33.0	37.0
7	35.84115456280453	37.0	35.0	37.0	35.0	37.0
8	35.8692945636032	37.0	35.0	37.0	35.0	37.0
9	37.74673923935803	39.0	37.0	39.0	35.0	39.0
10	37.14688510805661	39.0	37.0	39.0	33.0	39.0
11	36.80405900750515	39.0	35.0	39.0	32.0	39.0
12	36.251107389048244	38.0	35.0	39.0	32.0	39.0
13	36.01716217570397	38.0	35.0	39.0	31.0	39.0
14	37.04585445830046	39.0	35.0	41.0	31.0	41.0
15	37.211914217752735	39.0	35.0	41.0	32.0	41.0
16	37.26832456689579	39.0	35.0	41.0	32.0	41.0
17	37.18908004017417	39.0	35.0	41.0	32.0	41.0
18	37.111565443886136	39.0	35.0	41.0	32.0	41.0
19	37.0591257974369	39.0	35.0	41.0	32.0	41.0
20	36.93297951132479	38.0	35.0	41.0	32.0	41.0
21	36.81559815584594	38.0	35.0	41.0	31.0	41.0
22	36.6904381410481	38.0	35.0	41.0	31.0	41.0
23	36.577847999692594	38.0	35.0	40.0	31.0	41.0
24	36.46335312657048	38.0	35.0	40.0	31.0	41.0
25	36.31686584223728	38.0	35.0	40.0	30.0	41.0
26	36.14416513903864	38.0	35.0	40.0	30.0	41.0
27	36.01863141104609	38.0	35.0	40.0	30.0	41.0
28	35.90275771706445	38.0	35.0	40.0	30.0	41.0
29	36.0277158061845	38.0	35.0	40.0	30.0	41.0
30	36.00113922248066	38.0	35.0	40.0	30.0	41.0
31	35.894933850504174	38.0	35.0	40.0	30.0	41.0
32	35.748671095469405	38.0	34.0	40.0	29.0	41.0
33	35.5630885888635	38.0	34.0	40.0	29.0	41.0
34	35.36409309376509	38.0	34.0	40.0	27.0	41.0
35	35.20796235140945	37.0	34.0	40.0	27.0	41.0
36	35.065429947235614	37.0	34.0	40.0	26.0	41.0
37	34.945358007445634	37.0	34.0	40.0	26.0	41.0
38	34.87255174533857	37.0	34.0	40.0	26.0	41.0
39	34.79739922740825	37.0	34.0	40.0	25.0	41.0
40	34.651755811579235	37.0	33.0	40.0	24.0	41.0
41	34.55237748649245	36.0	33.0	40.0	24.0	41.0
42	34.474346774199624	36.0	33.0	40.0	24.0	41.0
43	34.336482771143615	36.0	33.0	40.0	24.0	41.0
44	34.22110409645417	36.0	33.0	40.0	23.0	41.0
45	34.20016636264797	36.0	33.0	40.0	23.0	41.0
46	34.179349934939246	36.0	33.0	40.0	23.0	41.0
47	34.12850309029167	35.0	33.0	40.0	23.0	41.0
48	34.015029900822846	35.0	33.0	40.0	23.0	41.0
49	33.94581158676664	35.0	33.0	40.0	24.0	41.0
50	33.828464890172775	35.0	33.0	39.0	24.0	41.0
51	33.579546285057276	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	5.0
12	9.0
13	8.0
14	19.0
15	52.0
16	124.0
17	263.0
18	629.0
19	1354.0
20	2194.0
21	3601.0
22	5301.0
23	7643.0
24	11022.0
25	15450.0
26	20213.0
27	24197.0
28	27228.0
29	31173.0
30	36897.0
31	44254.0
32	54899.0
33	71263.0
34	117209.0
35	153320.0
36	107287.0
37	134133.0
38	183359.0
39	273989.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.06261730337299	24.29497423563973	28.044086101711773	14.598322359275508
2	32.453148345301955	25.454238593271207	28.535036742185365	13.557576319241482
3	30.508784897164826	25.399989903716108	30.07667901577808	14.01454618334098
4	27.279104233582803	27.658317642653333	29.77823587782291	15.284342245940955
5	24.183010968030192	32.105127932725594	28.592600629435488	15.11926046980872
6	22.851507021061302	40.898765164204	25.636348430291566	10.613379384443133
7	87.68162951008159	3.9788399972574275	6.393961518089301	1.9455689745716802
8	88.69811433062014	3.0626399069936356	6.177042105271089	2.06220365711513
9	83.42288134380031	4.958254879933334	8.068739117298476	3.5501246589678734
10	47.73703851882994	24.263781239145555	14.257761141512981	13.741419100511521
11	37.744505248183984	24.659043218876135	20.852367465553968	16.744084067385913
12	34.01317489702167	21.47750826727425	26.7540221259308	17.755294709773278
13	23.95991323223487	29.341307890697934	27.43122660054354	19.267552276523652
14	20.813639928843802	31.324850947958176	28.579565874861835	19.281943248336187
15	20.09017337730491	23.26319429846273	38.10895095843119	18.537681365801173
16	22.019693781216542	20.326456558478203	36.55819189117713	21.095657769128128
17	22.149363218333644	21.083828540989032	27.89648445888062	28.870323781796703
18	24.835577496136665	23.026534390278634	29.724966678495896	22.412921435088805
19	29.480922920900138	23.836196081888396	25.008193812484887	21.674687184726583
20	31.38384639784934	22.805320289537313	24.177209372063885	21.633623940549466
21	27.275412308876966	24.584149889129243	25.916256599315414	22.224181202678377
22	26.222611004497367	23.90106847314803	24.88334648110601	24.992974041248594
23	25.291115797595126	26.408337420821397	24.957787738439944	23.34275904314353
24	24.586334905791876	23.406651944175085	30.914670578599946	21.092342571433093
25	23.164717857839804	24.101261206686754	29.24042039720589	23.493600538267554
26	22.513582892374366	27.806823430310402	25.90171493669856	23.77787874061667
27	23.374328766648507	26.443146996619255	27.251829198000937	22.930695038731304
28	21.10839114209314	26.400350808192456	30.965528725057844	21.525729324656556
29	21.961301094542655	24.35690815621513	30.585712552770033	23.096078196472178
30	23.516882267534946	25.331726969359288	28.509343960048856	22.642046803056914
31	27.394533389691695	25.355536116441797	24.403546960152077	22.84638353371443
32	28.71405741771717	24.859537334023496	24.931944265499116	21.49446098276022
33	26.674306690445675	25.592572751636688	24.66589965047268	23.067220907444955
34	23.23139853875127	25.875193355138293	27.455713856245495	23.437694249864943
35	23.902726071995545	25.026050672796767	27.54906680952155	23.522156445686136
36	28.004680456382168	26.47004530519032	24.374162253309738	21.151111985117776
37	24.712915181420428	28.775087193466646	25.741605957108877	20.77039166800405
38	24.535175377725338	28.480335980217315	23.962098248897508	23.022390393159842
39	24.05236204068501	27.883148322698332	25.564770298239704	22.499719338376956
40	25.212756579348884	25.621053313653114	25.627683709043183	23.538506397954826
41	21.50953006319219	24.950253198223958	26.653059287036594	26.887157451547257
42	24.449130928458786	26.58381686245169	24.07617118776752	24.890881021321995
43	24.03691623324224	25.767223393843224	25.665883827938224	24.529976544976307
44	22.864466430232795	27.867325788244763	25.835260291993574	23.43294748952887
45	22.40177031556915	28.397305347037154	24.251725974799974	24.94919836259372
46	23.754747702153598	27.95969925129274	25.788018724839347	22.49753432171432
47	24.790294909438593	25.554523323545965	26.725164836903577	22.930016930111865
48	25.28094416830354	23.268845203624718	27.600301682990246	23.84990894508149
49	22.85632912679953	24.533141051867023	28.24676523352177	24.363764587811676
50	22.12314301838202	27.415102684481337	26.993846541005606	23.467907756131044
51	22.174603928057195	27.79002140562875	25.709885542799576	24.325489123514473
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	390.0
1	1071.0
2	1752.0
3	6308.5
4	10865.0
5	7861.5
6	4858.0
7	4837.0
8	4816.0
9	4891.5
10	4967.0
11	5042.5
12	5118.0
13	4980.5
14	4843.0
15	4824.0
16	4805.0
17	4602.5
18	4400.0
19	4264.5
20	4129.0
21	4463.0
22	4797.0
23	4816.5
24	4836.0
25	5629.0
26	8123.0
27	9824.0
28	11488.5
29	13153.0
30	15154.5
31	17156.0
32	18743.0
33	20330.0
34	24625.0
35	28920.0
36	29321.0
37	29722.0
38	34312.5
39	38903.0
40	48445.5
41	57988.0
42	69328.0
43	80668.0
44	85526.5
45	90385.0
46	94714.0
47	99043.0
48	96466.0
49	93889.0
50	92902.5
51	91916.0
52	88554.0
53	85192.0
54	82312.5
55	79433.0
56	78121.5
57	76810.0
58	74471.5
59	72133.0
60	69759.0
61	67385.0
62	61887.5
63	56390.0
64	49977.5
65	43565.0
66	38294.0
67	33023.0
68	29126.5
69	25230.0
70	21551.0
71	17872.0
72	15201.0
73	12530.0
74	10364.0
75	6603.5
76	5009.0
77	4020.5
78	3032.0
79	2176.5
80	1321.0
81	971.0
82	621.0
83	475.0
84	329.0
85	223.0
86	117.0
87	93.5
88	70.0
89	55.0
90	40.0
91	26.5
92	13.0
93	9.5
94	6.0
95	4.5
96	3.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1327221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.35661703770259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34058510319826	17.597011936518424
2	5.771390392634888	2.6960031035169814
3	2.2775114033244273	1.5958488493934775
4	1.3909683692587838	1.2995326204934037
5	1.0504620941654965	1.2267620423023289
6	0.853479216274362	1.196063232249529
7	0.7386887880536795	1.2077289792830115
8	0.6466338841403897	1.2082543996375383
9	0.6211726847496766	1.3057643260781777
>10	10.673104401697925	59.81971734466261
>50	0.5951388012366683	8.392520306273449
>100	0.038270266899469774	1.5339614703181033
>500	9.729728872396721E-4	0.12991328007594014
>1k	0.0012972971829862295	0.38027094226310176
>5k	3.2432429574655736E-4	0.4106471669339193
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5421	0.4084474251085539	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1688	0.127183038845829	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027425726386185872	0.0
2	0.0	0.0	0.0	0.0872499757011078	0.0
3	0.0	0.0	0.0	0.15536221925361338	0.0
4	0.0	0.0	0.0	0.2591881834298885	0.0
5	0.0	0.0	0.0	0.460059025588052	0.0
6	0.0	0.0	0.0	0.8141824157393531	0.0
7	7.53454021598513E-5	0.0	0.0	0.9953881079337955	0.0
8	7.53454021598513E-5	0.0	0.0	1.4198841037023977	0.0
9	7.53454021598513E-5	0.0	0.0	1.6539822682130556	0.0
10	7.53454021598513E-5	0.0	0.0	1.9656108515462007	0.0
11	7.53454021598513E-5	0.0	0.0	2.2439367671246915	0.0
12	7.53454021598513E-5	0.0	0.0	2.464096032235777	0.0
13	7.53454021598513E-5	0.0	0.0	2.5735729015740407	0.0
14	7.53454021598513E-5	0.0	0.0	2.6300819531939292	0.0
15	7.53454021598513E-5	0.0	0.0	2.6958584892794795	0.0
16	7.53454021598513E-5	0.0	0.0	2.81226713561645	0.0
17	7.53454021598513E-5	0.0	0.0	2.941258464114115	0.0
18	7.53454021598513E-5	0.0	0.0	3.106566276452829	0.0
19	7.53454021598513E-5	0.0	0.0	3.2010494107612826	7.53454021598513E-5
20	7.53454021598513E-5	0.0	0.0	3.299525851384208	7.53454021598513E-5
21	7.53454021598513E-5	0.0	0.0	3.42512663678468	7.53454021598513E-5
22	7.53454021598513E-5	0.0	0.0	3.563536140552327	7.53454021598513E-5
23	7.53454021598513E-5	0.0	0.0	3.7179942149800222	7.53454021598513E-5
24	7.53454021598513E-5	0.0	0.0	3.8406565296962603	7.53454021598513E-5
25	7.53454021598513E-5	0.0	0.0	3.9453866386984533	7.53454021598513E-5
26	7.53454021598513E-5	0.0	0.0	4.041452026452264	7.53454021598513E-5
27	7.53454021598513E-5	0.0	0.0	4.142414865346464	7.53454021598513E-5
28	7.53454021598513E-5	0.0	0.0	4.25091224445665	7.53454021598513E-5
29	7.53454021598513E-5	0.0	0.0	4.364156383902907	7.53454021598513E-5
30	7.53454021598513E-5	0.0	0.0	4.49917534457336	7.53454021598513E-5
31	7.53454021598513E-5	0.0	0.0	4.624324057560873	7.53454021598513E-5
32	7.53454021598513E-5	0.0	0.0	4.757082656166531	7.53454021598513E-5
33	7.53454021598513E-5	0.0	0.0	4.881703951338926	7.53454021598513E-5
34	7.53454021598513E-5	0.0	0.0	5.013784441325145	7.53454021598513E-5
35	7.53454021598513E-5	0.0	0.0	5.161386084156294	7.53454021598513E-5
36	7.53454021598513E-5	0.0	0.0	5.303788894238412	7.53454021598513E-5
37	7.53454021598513E-5	0.0	0.0	5.446493085929171	7.53454021598513E-5
38	7.53454021598513E-5	0.0	0.0	5.591382294282565	7.53454021598513E-5
39	7.53454021598513E-5	0.0	0.0	5.751340583067929	7.53454021598513E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	40	6.8157533E-9	45.000004	1
ATTAGCG	65	0.0	41.53846	1
TATAGCG	105	0.0	40.714283	1
TACGGGA	305	0.0	39.836067	4
TTACGGG	410	0.0	39.512196	3
CGTTAGG	195	0.0	39.23077	2
CTATGCG	75	0.0	39.000004	1
CGTTTTT	4230	0.0	38.563828	1
GGGCGAT	3000	0.0	38.024998	7
GCGATAT	155	0.0	37.74194	9
ATAGGGC	670	0.0	37.611942	4
ATAGCGG	240	0.0	37.499996	2
CATAGCG	60	1.5643309E-10	37.499996	1
TCGTAAG	30	1.14001734E-4	37.499996	1
CCGACAA	30	1.14001734E-4	37.499996	32
GCGTTAG	85	0.0	37.058826	1
AGGGATC	775	0.0	36.870968	6
TATACGG	160	0.0	36.562504	2
ACGGGTA	105	0.0	36.42857	5
AGGGCGA	1495	0.0	36.421406	6
>>END_MODULE