FastQCFastQC Report
Thu 26 May 2016
SRR1545342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545342_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3108853
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99770.32092221793696907No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41560.13368274408600214No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT38400.12351822360208091No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAA4850.040.82474511
GCTACGA4900.040.40816510
TAATGCG950.040.263161
CTTAACG403.4607547E-739.3750041
CGAATAT5050.039.20792414
TACGGGA7450.038.3557054
TGCGTAG2950.038.1355931
ATAGGGC15500.037.5967754
TATACGG3300.037.52
CGTAGAA301.1403698E-437.49999640
GTAACGT301.1403698E-437.49999633
TACGAAA9850.037.4619320
CATACGA9850.037.4619318
CGTTTTT76800.037.353511
ATAACGG2350.037.3404272
TAAGGGA22450.037.1826254
TACGAAT5350.037.00934612
TGACTAT5350.036.16822423
AACGGGA8550.036.052634
GGGCGAT72650.036.0495537
Produced by FastQC (version 0.11.5)