##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545342_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3108853
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166070573295038	31.0	31.0	33.0	30.0	33.0
2	31.525729585799006	31.0	31.0	34.0	30.0	34.0
3	31.705474977427365	31.0	31.0	34.0	30.0	34.0
4	35.50259082690626	37.0	35.0	37.0	33.0	37.0
5	35.411891459647656	37.0	35.0	37.0	33.0	37.0
6	35.464437848943	37.0	35.0	37.0	33.0	37.0
7	35.8010996338521	37.0	35.0	37.0	35.0	37.0
8	35.830211013515274	37.0	35.0	37.0	35.0	37.0
9	37.70991327026398	39.0	37.0	39.0	35.0	39.0
10	37.074466370716145	39.0	37.0	39.0	33.0	39.0
11	36.7091525395379	39.0	35.0	39.0	32.0	39.0
12	36.086754825654346	38.0	35.0	39.0	32.0	39.0
13	35.82154479481661	38.0	35.0	39.0	31.0	39.0
14	36.81314362563943	39.0	35.0	41.0	31.0	41.0
15	37.01906909075469	39.0	35.0	41.0	32.0	41.0
16	37.09013292040505	39.0	35.0	41.0	32.0	41.0
17	37.01519113319285	38.0	35.0	41.0	32.0	41.0
18	36.94820308325932	38.0	35.0	41.0	31.0	41.0
19	36.903571510135734	38.0	35.0	41.0	31.0	41.0
20	36.77825101412	38.0	35.0	41.0	31.0	41.0
21	36.664103449085566	38.0	35.0	41.0	31.0	41.0
22	36.53194538307215	38.0	35.0	40.0	31.0	41.0
23	36.43488804391845	38.0	35.0	40.0	31.0	41.0
24	36.32640945068808	38.0	35.0	40.0	30.0	41.0
25	36.187587190516886	38.0	35.0	40.0	30.0	41.0
26	36.003294784282176	38.0	35.0	40.0	30.0	41.0
27	35.8514899224891	38.0	34.0	40.0	30.0	41.0
28	35.73577007340006	38.0	34.0	40.0	29.0	41.0
29	35.87158929675993	38.0	34.0	40.0	30.0	41.0
30	35.86474111191491	38.0	35.0	40.0	30.0	41.0
31	35.76124120374942	38.0	34.0	40.0	29.0	41.0
32	35.64194897603714	38.0	34.0	40.0	29.0	41.0
33	35.45167687246711	37.0	34.0	40.0	28.0	41.0
34	35.27999619152144	37.0	34.0	40.0	27.0	41.0
35	35.15991846510594	37.0	34.0	40.0	27.0	41.0
36	35.00852565238691	37.0	34.0	40.0	26.0	41.0
37	34.90481923719134	37.0	33.0	40.0	26.0	41.0
38	34.83895732606206	37.0	33.0	40.0	26.0	41.0
39	34.769455487281	37.0	33.0	40.0	25.0	41.0
40	34.6180581712934	36.0	33.0	40.0	24.0	41.0
41	34.53655287014214	36.0	33.0	40.0	24.0	41.0
42	34.45689326578002	36.0	33.0	40.0	24.0	41.0
43	34.33800568891485	36.0	33.0	40.0	24.0	41.0
44	34.20402798073759	36.0	33.0	40.0	23.0	41.0
45	34.16869983881515	35.0	33.0	40.0	23.0	41.0
46	34.15674977234369	35.0	33.0	40.0	23.0	41.0
47	34.11761025690182	35.0	33.0	40.0	23.0	41.0
48	34.01220224951131	35.0	33.0	40.0	23.0	41.0
49	33.963094427430306	35.0	33.0	39.0	24.0	41.0
50	33.83849348939947	35.0	33.0	39.0	24.0	41.0
51	33.59667986874902	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	4.0
12	10.0
13	13.0
14	58.0
15	111.0
16	324.0
17	777.0
18	1704.0
19	3405.0
20	5678.0
21	9057.0
22	13403.0
23	18561.0
24	26468.0
25	36450.0
26	47622.0
27	57785.0
28	65345.0
29	75258.0
30	89170.0
31	107529.0
32	132304.0
33	169048.0
34	277793.0
35	376453.0
36	246660.0
37	305989.0
38	418385.0
39	623171.0
40	312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.311593375434605	23.270125670142654	28.80901734498222	13.609263609440521
2	31.270793440539002	25.74348159916213	29.427669947726702	13.558055012572162
3	29.891828272356396	25.403806484256414	30.46139524770068	14.242969995686511
4	27.13470209109276	28.1104960575492	29.49026538083338	15.264536470524659
5	24.29201380702143	32.63846827109548	28.348365136595394	14.721152785287691
6	22.871586401801565	41.20194811398287	24.973358341484786	10.953107142730776
7	87.99139103714457	4.167228234979267	5.906937381728889	1.9344433461472768
8	88.87747346046918	3.3736236483358972	5.619403683609357	2.129499207585563
9	83.99895395504386	5.010722604124415	7.53075169523937	3.4595717455923456
10	49.24780940108779	23.368393423555247	13.294356471663344	14.089440703693612
11	39.51338323169349	23.067542916953617	20.117869838168613	17.301204013184286
12	34.89486315370974	21.803443263480133	25.80942231749137	17.492271265318752
13	23.418926530138286	31.062806765067375	26.438014277291334	19.080252427503005
14	20.337758009143563	32.694244468940795	27.914539542397147	19.05345797951849
15	19.771504152817776	22.94544000633031	39.08489722736971	18.198158613482207
16	21.16047301046399	19.461261114629735	37.627800349517976	21.7504655253883
17	21.663874104050592	20.354227105623842	27.711667293371544	30.27023149695402
18	24.60402598643294	22.58424570090641	30.119983157775554	22.691745154885098
19	29.967933511169555	23.792601322738644	24.30330414464756	21.936161021444242
20	31.529988712878993	22.311572789064005	24.433352107674438	21.72508639038256
21	26.91484608632187	24.59357197011245	25.873851224229643	22.617730719336038
22	25.74531507279373	23.717396737639252	24.47452484887513	26.06276334069189
23	24.572824768491788	26.757971509106415	24.592156657133675	24.077047065268122
24	24.334762692221215	23.27597991928213	31.66203098055778	20.727226407938875
25	23.26285610802441	23.86973588008182	29.479940029329143	23.38746798256463
26	21.91637880594547	28.557702792637667	25.713985190036325	23.81193321138053
27	22.83388761063968	27.337831669750866	27.099158435603098	22.729122284006355
28	20.953193991481747	26.643556321254174	31.76090989184757	20.642339795416508
29	21.86455905119991	23.540321784272205	30.97611884511748	23.619000319410407
30	23.364340481843303	26.146041643011102	28.108405254285103	22.38121262086049
31	28.126900821621348	25.3281837385042	24.091168028851797	22.45374741102265
32	28.922724876345068	24.838710611276895	24.87197046627808	21.36659404609996
33	27.469970436041848	25.433946217463482	24.217581210819553	22.87850213567512
34	22.241772126247206	25.563415188817224	27.654604447363706	24.540208237571864
35	23.45672181991236	24.190368602182218	28.397161268159028	23.95574830974639
36	28.324208317344052	25.968709360011555	23.960412409335532	21.746669913308864
37	24.012135665468904	28.805382563923093	26.170552290507143	21.01192948010086
38	23.68352572476087	29.29443753049758	24.09181135293306	22.93022539180849
39	23.629872496383715	28.129602782762646	25.13052241453681	23.110002306316833
40	24.6499271596309	25.441408776806107	25.426451491916797	24.482212571646198
41	20.880112375850516	24.448534555992193	26.088914464595142	28.58243860356215
42	23.25082594770483	26.02442122544874	24.37989187652166	26.344860950324765
43	23.58763827044894	25.338090929355616	25.963015941892397	25.111254858303045
44	22.914656949041976	26.55863754252774	26.34775590869044	24.17894959973984
45	21.62778362309186	28.840829720800564	24.477194643812364	25.05419201229521
46	23.07339716609309	28.652946922868338	25.51535887994704	22.75829703109153
47	23.894729020638803	25.419825253879807	27.041194935881496	23.644250789599894
48	23.9822854280984	23.104855713666744	28.337267796193643	24.57559106204121
49	22.402828309990856	23.955330149093573	28.81316678530635	24.828674755609224
50	21.521860313112263	27.732414494992202	27.440956519976982	23.30476867191855
51	21.732452451112998	28.47014638517807	24.744624464392494	25.052776699316436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	903.0
1	2645.5
2	4388.0
3	12054.5
4	19721.0
5	14670.5
6	9620.0
7	9371.5
8	9123.0
9	9303.0
10	9483.0
11	9467.0
12	9451.0
13	9652.5
14	9854.0
15	9634.0
16	9414.0
17	9045.0
18	8676.0
19	8748.0
20	8820.0
21	9184.5
22	9549.0
23	10221.5
24	10894.0
25	12503.0
26	16329.5
27	18547.0
28	23571.5
29	28596.0
30	34664.5
31	40733.0
32	45129.0
33	49525.0
34	55923.0
35	62321.0
36	67221.0
37	72121.0
38	83373.0
39	94625.0
40	119910.5
41	145196.0
42	178450.0
43	211704.0
44	222319.0
45	232934.0
46	233587.0
47	234240.0
48	231881.0
49	229522.0
50	221903.0
51	214284.0
52	203529.0
53	192774.0
54	185504.5
55	178235.0
56	175682.5
57	173130.0
58	171155.0
59	169180.0
60	163018.0
61	156856.0
62	143301.5
63	129747.0
64	115753.5
65	101760.0
66	89804.0
67	77848.0
68	67128.0
69	56408.0
70	48266.5
71	40125.0
72	33021.0
73	25917.0
74	22038.0
75	14505.5
76	10852.0
77	8023.5
78	5195.0
79	3763.0
80	2331.0
81	1664.5
82	998.0
83	796.5
84	595.0
85	443.0
86	291.0
87	166.5
88	42.0
89	33.5
90	25.0
91	19.0
92	13.0
93	9.0
94	5.0
95	6.5
96	8.0
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3108853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.49081219283945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.85109282099761	14.765115607460572
2	7.238905169230988	2.677064719320498
3	2.338460640095613	1.2972010954906532
4	1.1188963267051084	0.8275720736144838
5	0.6845597339147103	0.6329032737298528
6	0.47680280862021196	0.5289882712328826
7	0.36247823207946667	0.46917618393617233
8	0.28763849491716137	0.42549355111553894
9	0.24761832811117718	0.41207976005478075
>10	3.9436944964167413	19.132036412890322
>50	2.4460383320034054	33.21775294070552
>100	0.9969621340622001	23.916274418379704
>500	0.004744026585439305	0.6241179918247584
>1k	0.0019327515718456424	0.7500787130274958
>5k	1.7570468834960385E-4	0.3241449872168461
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9977	0.32092221793696907	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4156	0.13368274408600214	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3840	0.12351822360208091	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.21662040630419E-5	0.0	0.0	0.037409295325317726	0.0
2	3.21662040630419E-5	0.0	0.0	0.1318814366584718	0.0
3	3.21662040630419E-5	0.0	0.0	0.2004597837208771	0.0
4	3.21662040630419E-5	0.0	0.0	0.3254254865057949	0.0
5	3.21662040630419E-5	0.0	0.0	0.5869045593342626	0.0
6	3.21662040630419E-5	0.0	0.0	0.935007219704502	0.0
7	3.21662040630419E-5	0.0	0.0	1.1163924444159952	0.0
8	3.21662040630419E-5	0.0	0.0	1.5427554792716156	0.0
9	3.21662040630419E-5	0.0	0.0	1.727260825777224	0.0
10	3.21662040630419E-5	0.0	0.0	1.9988400866814866	0.0
11	3.21662040630419E-5	0.0	0.0	2.279265053703086	0.0
12	3.21662040630419E-5	0.0	0.0	2.5082240942238183	0.0
13	3.21662040630419E-5	0.0	0.0	2.6148228944887393	0.0
14	3.21662040630419E-5	0.0	0.0	2.6662566547855433	0.0
15	3.21662040630419E-5	0.0	0.0	2.7331623592366703	0.0
16	3.21662040630419E-5	0.0	0.0	2.8777172802959807	0.0
17	3.21662040630419E-5	0.0	0.0	3.04076776869154	0.0
18	3.21662040630419E-5	0.0	0.0	3.2363704555988977	3.21662040630419E-5
19	3.21662040630419E-5	0.0	0.0	3.344867061903538	6.43324081260838E-5
20	3.21662040630419E-5	0.0	0.0	3.4550041446153936	6.43324081260838E-5
21	3.21662040630419E-5	0.0	0.0	3.598980074001569	6.43324081260838E-5
22	3.21662040630419E-5	0.0	0.0	3.7618375651727503	6.43324081260838E-5
23	3.21662040630419E-5	0.0	0.0	3.945924751025539	6.43324081260838E-5
24	3.21662040630419E-5	0.0	0.0	4.098231727264042	6.43324081260838E-5
25	6.43324081260838E-5	0.0	0.0	4.223004432824582	6.43324081260838E-5
26	6.43324081260838E-5	0.0	0.0	4.342566213326909	6.43324081260838E-5
27	6.43324081260838E-5	0.0	0.0	4.459072204443246	6.43324081260838E-5
28	6.43324081260838E-5	0.0	0.0	4.583555414167218	6.43324081260838E-5
29	6.43324081260838E-5	0.0	0.0	4.716337504539456	6.43324081260838E-5
30	6.43324081260838E-5	0.0	0.0	4.8757210456718285	6.43324081260838E-5
31	6.43324081260838E-5	0.0	0.0	5.029025174236286	6.43324081260838E-5
32	6.43324081260838E-5	0.0	0.0	5.1756708985596935	6.43324081260838E-5
33	6.43324081260838E-5	0.0	0.0	5.324857753004082	6.43324081260838E-5
34	6.43324081260838E-5	0.0	0.0	5.47816188156854	6.43324081260838E-5
35	6.43324081260838E-5	0.0	0.0	5.65803529468907	6.43324081260838E-5
36	6.43324081260838E-5	0.0	0.0	5.817676165453947	6.43324081260838E-5
37	6.43324081260838E-5	0.0	0.0	5.979343507074796	6.43324081260838E-5
38	6.43324081260838E-5	0.0	0.0	6.140303192206257	6.43324081260838E-5
39	6.43324081260838E-5	0.0	0.0	6.302388694479926	6.43324081260838E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGAA	485	0.0	40.824745	11
GCTACGA	490	0.0	40.408165	10
TAATGCG	95	0.0	40.26316	1
CTTAACG	40	3.4607547E-7	39.375004	1
CGAATAT	505	0.0	39.207924	14
TACGGGA	745	0.0	38.355705	4
TGCGTAG	295	0.0	38.135593	1
ATAGGGC	1550	0.0	37.596775	4
TATACGG	330	0.0	37.5	2
CGTAGAA	30	1.1403698E-4	37.499996	40
GTAACGT	30	1.1403698E-4	37.499996	33
TACGAAA	985	0.0	37.46193	20
CATACGA	985	0.0	37.46193	18
CGTTTTT	7680	0.0	37.35351	1
ATAACGG	235	0.0	37.340427	2
TAAGGGA	2245	0.0	37.182625	4
TACGAAT	535	0.0	37.009346	12
TGACTAT	535	0.0	36.168224	23
AACGGGA	855	0.0	36.05263	4
GGGCGAT	7265	0.0	36.049553	7
>>END_MODULE