FastQCFastQC Report
Thu 26 May 2016
SRR1545341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545341_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2047973
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC63590.31050214040907764No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.22656548694733766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTA351.2125565E-745.00000433
ACGCGAT253.891878E-545.011
GAATCGT207.034581E-445.09
ATTCGAC253.891878E-545.011
TAACGCG207.034581E-445.01
TTAACGC207.034581E-445.027
ACTCGAT253.891878E-545.014
TCGAATA207.034581E-445.041
AAGTACG1100.040.9090921
CACGACC3050.039.83606727
CGTTAGG3450.039.7826082
CGTTGAT1700.039.70588325
GACGGGT2400.039.3750044
AAATCCG403.4599907E-739.37542
CGACCGA403.4599907E-739.37534
CGTTTTT47300.039.244191
AACACGT3050.038.36065741
GCGAGAC3000.038.2521
CGGTCTA1300.038.0769231
TAGCGGG14850.037.8787883
Produced by FastQC (version 0.11.5)