##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545341_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2047973
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.318590137662948	31.0	31.0	33.0	30.0	34.0
2	31.692077971731074	31.0	31.0	34.0	30.0	34.0
3	31.854497593474132	31.0	31.0	34.0	30.0	34.0
4	35.64136050621761	37.0	35.0	37.0	33.0	37.0
5	35.567371737810994	37.0	35.0	37.0	33.0	37.0
6	35.62329581493506	37.0	35.0	37.0	33.0	37.0
7	35.93639808727947	37.0	35.0	37.0	35.0	37.0
8	35.98212720577859	37.0	35.0	37.0	35.0	37.0
9	37.87808384192565	39.0	38.0	39.0	35.0	39.0
10	37.2930346249682	39.0	37.0	39.0	34.0	39.0
11	36.92484080600672	39.0	37.0	39.0	33.0	39.0
12	36.0568982110604	37.0	35.0	39.0	32.0	39.0
13	35.63990394404614	37.0	35.0	39.0	31.0	39.0
14	36.54279084734027	38.0	35.0	41.0	31.0	41.0
15	36.85507523780831	38.0	35.0	41.0	32.0	41.0
16	36.99812790500656	38.0	35.0	41.0	32.0	41.0
17	36.960725556440444	38.0	35.0	41.0	32.0	41.0
18	36.910046665654285	38.0	35.0	40.0	32.0	41.0
19	36.88236417179328	38.0	35.0	41.0	32.0	41.0
20	36.755291207452444	38.0	35.0	40.0	32.0	41.0
21	36.60767842154169	38.0	35.0	40.0	31.0	41.0
22	36.509433962264154	38.0	35.0	40.0	31.0	41.0
23	36.464392352828874	38.0	35.0	40.0	31.0	41.0
24	36.359782086970874	38.0	35.0	40.0	31.0	41.0
25	36.258744133833794	38.0	35.0	40.0	31.0	41.0
26	36.09356129206782	37.0	35.0	40.0	31.0	41.0
27	35.987802085281395	37.0	35.0	40.0	30.0	41.0
28	35.92707276902576	37.0	35.0	40.0	30.0	41.0
29	36.053613011499664	37.0	35.0	40.0	30.0	41.0
30	36.06069904241902	37.0	35.0	40.0	30.0	41.0
31	35.92282661929625	37.0	35.0	40.0	30.0	41.0
32	35.78811585894931	37.0	34.0	40.0	30.0	41.0
33	35.625412542059884	37.0	34.0	40.0	30.0	41.0
34	35.48642291670838	37.0	34.0	40.0	29.0	41.0
35	35.352311285353856	37.0	34.0	40.0	29.0	41.0
36	35.18285104344638	37.0	34.0	40.0	27.0	41.0
37	35.054522203173576	36.0	34.0	40.0	27.0	41.0
38	35.01505781570363	36.0	34.0	40.0	27.0	41.0
39	34.96612455339987	36.0	34.0	40.0	27.0	41.0
40	34.80900334135264	36.0	34.0	40.0	26.0	41.0
41	34.78629991703992	36.0	34.0	40.0	26.0	41.0
42	34.74629987797691	36.0	34.0	40.0	27.0	41.0
43	34.62371427748315	35.0	33.0	40.0	26.0	41.0
44	34.48347268250119	35.0	33.0	40.0	26.0	41.0
45	34.45645181845659	35.0	33.0	40.0	26.0	41.0
46	34.48918613673129	35.0	34.0	40.0	26.0	41.0
47	34.463229739845204	35.0	33.0	40.0	26.0	41.0
48	34.39555160151037	35.0	34.0	40.0	26.0	41.0
49	34.38030725991017	35.0	34.0	40.0	26.0	41.0
50	34.255017033915976	35.0	33.0	39.0	26.0	41.0
51	34.02002711949816	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	5.0
12	7.0
13	10.0
14	18.0
15	65.0
16	152.0
17	344.0
18	792.0
19	1556.0
20	2716.0
21	4532.0
22	6847.0
23	9991.0
24	14581.0
25	20518.0
26	27582.0
27	32942.0
28	37627.0
29	43546.0
30	53477.0
31	66771.0
32	84442.0
33	113935.0
34	206747.0
35	300872.0
36	148847.0
37	187216.0
38	264374.0
39	417218.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.707049360514034	23.664765111649423	28.692663428668247	12.9355220991683
2	32.10017905509496	25.231192012785325	29.35834603288227	13.310282899237441
3	29.749903929397508	25.372990757202363	30.576379669067904	14.300725644332225
4	26.360992063860216	28.39671226134329	29.216156658315317	16.026139016481174
5	24.00637117774502	31.89407282224912	28.569566102678113	15.529989897327749
6	22.27807690824049	40.880177619529164	25.8088851757323	11.03286029649805
7	88.88183584451554	4.250446661162037	5.341916128777088	1.5258013655453464
8	90.5172089671104	2.7848023387027077	4.984831342991338	1.7131573511955478
9	86.05767751820946	4.242634058163853	6.921673283778644	2.7780151398480353
10	52.63658261119654	21.382264316961212	13.15544687356718	12.825706198275075
11	45.61554278303473	22.231982550551205	18.31713601692991	13.83533864948415
12	39.91795790276532	21.923531218429147	23.32442859354103	14.834082285264502
13	23.893967352108646	36.99247988132656	24.056567152008352	15.056985614556442
14	18.836381143696716	39.03884475039466	26.822765729821636	15.30200837608699
15	17.043828214532127	23.16910428018338	44.44536134021298	15.34170616507151
16	19.655532568056316	18.469970063081888	42.568236983593046	19.30626038526875
17	20.633084518204097	19.206307895660736	26.77999172840658	33.38061585772859
18	23.984691204425058	23.362173231776005	31.519556166023673	21.13357939777526
19	30.736977489449323	23.378042581616064	24.580548669342807	21.3044312595918
20	31.866631054217997	24.116187078638244	23.148059080857024	20.869122786286734
21	24.76033619583852	26.639657847051694	27.432832366442334	21.167173590667453
22	24.801694162960157	24.61746321850923	25.40765918300681	25.17318343552381
23	22.36157410278358	29.486668037127444	24.372831087128592	23.778926772960386
24	22.413918542871414	24.98421610050523	33.832867913785975	18.76899744283738
25	20.564480098126296	24.737289016993877	32.56991181036078	22.128319074519048
26	19.88185391115996	30.916081413182695	26.73199304873648	22.470071626920863
27	21.330505822098242	30.113629427731713	27.768725466595505	20.78713928357454
28	18.549219154744716	27.060317689735168	35.1757078828676	19.21475527265252
29	19.055134027645874	24.898765755212594	34.75133705375998	21.29476316338155
30	21.836225379924443	27.224333523928294	30.21192173920262	20.727519356944647
31	28.67889371588395	25.641402498958726	24.474736727486153	21.20496705767117
32	30.226082082136823	25.69965522006394	25.06297690448067	19.011285793318564
33	28.260479996562456	25.675387322000827	25.174013524592365	20.890119156844353
34	22.221337878966178	25.677291643981636	28.806483288598045	23.294887188454144
35	22.055320065254765	25.566596825251114	29.903519235849302	22.47456387364482
36	30.51246281078901	23.2647598381424	26.0975608565152	20.125216494553396
37	23.83698417899064	28.641637365336358	27.81843315317145	19.702945302501547
38	23.381948883115157	30.463096925594236	23.148498539775673	23.006455651514937
39	22.948007615334774	28.927188004919984	26.625985791804872	21.49881858794037
40	25.07567238435272	25.247940280462682	24.96468459300977	24.711702742174822
41	19.939081228121662	23.79875125306828	26.2365275323454	30.025639986464665
42	23.812472137083837	26.000586921800238	23.939719908416762	26.247221032699166
43	23.490251092177488	26.364263591365706	25.444817876016916	24.70066744043989
44	22.343702773425235	29.41884487734946	26.708994698660575	21.52845765056473
45	19.49840159025534	32.08767889029787	24.103198626153763	24.310720893293027
46	22.94659158104135	29.94985773738228	26.12798117943938	20.975569502136988
47	23.736836374307668	26.28022928036649	27.270720854230014	22.71221349109583
48	24.85799373331582	23.43009404909147	28.74325003308149	22.96866218451122
49	22.21186509783088	23.196008931758378	30.128033914509615	24.464092055901126
50	20.734794843486707	30.800601375115782	25.985401174722515	22.479202606674992
51	20.757549049718918	31.670730034038534	23.782588930615784	23.789131985626764
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	420.0
1	1355.5
2	2291.0
3	5874.0
4	9457.0
5	7130.0
6	4803.0
7	5036.5
8	5270.0
9	5704.5
10	6139.0
11	6371.5
12	6604.0
13	6494.5
14	6385.0
15	6198.5
16	6012.0
17	5941.0
18	5870.0
19	5770.0
20	5670.0
21	6572.0
22	7474.0
23	7561.0
24	7648.0
25	8268.5
26	11950.5
27	15012.0
28	16107.5
29	17203.0
30	20615.0
31	24027.0
32	29413.0
33	34799.0
34	39480.5
35	44162.0
36	47026.5
37	49891.0
38	61369.0
39	72847.0
40	97434.0
41	122021.0
42	147658.0
43	173295.0
44	177179.0
45	181063.0
46	173615.0
47	166167.0
48	160768.5
49	155370.0
50	157651.5
51	159933.0
52	154176.5
53	148420.0
54	132638.0
55	116856.0
56	110222.0
57	103588.0
58	96436.0
59	89284.0
60	83249.5
61	77215.0
62	69843.0
63	62471.0
64	53795.0
65	45119.0
66	39710.0
67	34301.0
68	29945.0
69	25589.0
70	21658.0
71	17727.0
72	14833.0
73	11939.0
74	9787.5
75	6166.0
76	4696.0
77	3212.5
78	1729.0
79	1438.5
80	1148.0
81	1011.0
82	874.0
83	654.0
84	434.0
85	288.0
86	142.0
87	90.0
88	38.0
89	32.0
90	26.0
91	18.0
92	10.0
93	7.5
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2047973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.353913125195145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23396055744779	13.923731812042824
2	7.23343765313761	2.5105689725813107
3	2.1037930420051567	1.0952712505304254
4	1.1246948013403875	0.7807142349927878
5	0.6368877330718314	0.5526247195115518
6	0.48082503617548855	0.5006517543724944
7	0.3690395764175964	0.4482996524236848
8	0.28953863476534575	0.40197026512843337
9	0.251577626634887	0.3929270649178147
>10	3.469657582738353	15.458593632092732
>50	2.40970781126007	31.190759192876712
>100	1.392065070905005	31.733438689111054
>500	0.0039651904357352035	0.4121813063761544
>1k	5.664557765336005E-4	0.2857154703034326
>5k	2.832278882668003E-4	0.3125519827386079
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6359	0.31050214040907764	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4640	0.22656548694733766	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03774463823497673	0.0
2	0.0	0.0	0.0	0.13095875775706028	0.0
3	0.0	0.0	0.0	0.19765885585405668	0.0
4	0.0	0.0	0.0	0.3251019422619341	0.0
5	0.0	0.0	0.0	0.5364328533628129	0.0
6	0.0	0.0	0.0	0.806504773256288	0.0
7	0.0	0.0	0.0	0.9448366750928845	0.0
8	0.0	0.0	0.0	1.3227225163612997	0.0
9	0.0	0.0	0.0	1.487617268391722	0.0
10	0.0	0.0	0.0	1.7429429001261247	0.0
11	0.0	0.0	0.0	2.0392846976009937	0.0
12	0.0	0.0	0.0	2.2765925136708347	0.0
13	0.0	0.0	0.0	2.3835763459772177	0.0
14	0.0	0.0	0.0	2.4251784569425476	0.0
15	0.0	0.0	0.0	2.4870445069344176	0.0
16	0.0	0.0	0.0	2.6277690184392077	0.0
17	0.0	0.0	0.0	2.7788452289165924	0.0
18	0.0	0.0	0.0	2.981191646569559	0.0
19	0.0	0.0	0.0	3.0857828692077485	0.0
20	0.0	0.0	0.0	3.197014804394394	0.0
21	0.0	0.0	0.0	3.341547959860799	0.0
22	0.0	0.0	0.0	3.486032286558465	0.0
23	0.0	0.0	0.0	3.6549798263941957	0.0
24	0.0	0.0	0.0	3.7819346251146864	0.0
25	0.0	0.0	0.0	3.890822779401877	0.0
26	0.0	0.0	0.0	3.999271474770419	0.0
27	0.0	0.0	0.0	4.109819807194723	0.0
28	0.0	0.0	0.0	4.2196357080879485	0.0
29	0.0	0.0	0.0	4.338289616122869	0.0
30	0.0	0.0	0.0	4.486436100475934	0.0
31	0.0	0.0	0.0	4.626672324293338	0.0
32	0.0	0.0	0.0	4.755824417607068	0.0
33	0.0	0.0	0.0	4.884244079389719	0.0
34	0.0	0.0	0.0	5.031316330830533	0.0
35	0.0	0.0	0.0	5.200898644659866	0.0
36	0.0	0.0	0.0	5.343771621989157	0.0
37	0.0	0.0	0.0	5.49704512705978	0.0
38	0.0	0.0	0.0	5.6587171803534515	0.0
39	0.0	0.0	0.0	5.889433112643575	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGTA	35	1.2125565E-7	45.000004	33
ACGCGAT	25	3.891878E-5	45.0	11
GAATCGT	20	7.034581E-4	45.0	9
ATTCGAC	25	3.891878E-5	45.0	11
TAACGCG	20	7.034581E-4	45.0	1
TTAACGC	20	7.034581E-4	45.0	27
ACTCGAT	25	3.891878E-5	45.0	14
TCGAATA	20	7.034581E-4	45.0	41
AAGTACG	110	0.0	40.909092	1
CACGACC	305	0.0	39.836067	27
CGTTAGG	345	0.0	39.782608	2
CGTTGAT	170	0.0	39.705883	25
GACGGGT	240	0.0	39.375004	4
AAATCCG	40	3.4599907E-7	39.375	42
CGACCGA	40	3.4599907E-7	39.375	34
CGTTTTT	4730	0.0	39.24419	1
AACACGT	305	0.0	38.360657	41
GCGAGAC	300	0.0	38.25	21
CGGTCTA	130	0.0	38.07692	31
TAGCGGG	1485	0.0	37.878788	3
>>END_MODULE