FastQCFastQC Report
Thu 26 May 2016
SRR1545340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545340_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3485657
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122170.3504934650770285No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43590.1250553339011842No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA207.0352625E-445.019
TAAGCGT406.8212103E-945.032
CGAATAT3050.042.7868814
TAATACG603.6379788E-1241.2500041
CGTTTTT99050.039.9798051
GCTACGA3400.039.04411710
CGTTAGG6450.038.023262
CGCAATA301.1403977E-437.50000445
CAATACG301.1403977E-437.5000041
GGCGATA15850.037.3343858
GGGCGAT84850.037.283447
GCGATCG1450.037.241389
GCGATAA3450.037.1739129
ATACCGG1400.036.9642832
GCGATAC1950.036.9230779
TTTACGG3200.036.56252
TGGGCGA22400.036.3616076
TTACGGG8350.036.1077843
CGAGGGA17950.036.1002774
ATTCGAC504.8838046E-836.00000412
Produced by FastQC (version 0.11.5)