##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545340_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3485657
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.182786200707643	31.0	31.0	33.0	30.0	34.0
2	31.553056425230594	31.0	31.0	34.0	30.0	34.0
3	31.71139587171084	31.0	31.0	34.0	30.0	34.0
4	35.521790296635615	37.0	35.0	37.0	33.0	37.0
5	35.428522657278094	37.0	35.0	37.0	33.0	37.0
6	35.485132645007816	37.0	35.0	37.0	33.0	37.0
7	35.84547131286871	37.0	35.0	37.0	35.0	37.0
8	35.88920194958942	37.0	35.0	37.0	35.0	37.0
9	37.77090430871425	39.0	37.0	39.0	35.0	39.0
10	37.08973917972996	39.0	37.0	39.0	33.0	39.0
11	36.75197961245183	39.0	35.0	39.0	32.0	39.0
12	36.17428536427996	38.0	35.0	39.0	32.0	39.0
13	35.93296357042589	38.0	35.0	39.0	31.0	39.0
14	36.9464448165726	39.0	35.0	41.0	31.0	41.0
15	37.128482234482625	39.0	35.0	41.0	32.0	41.0
16	37.18812206708807	39.0	35.0	41.0	32.0	41.0
17	37.10755074294459	39.0	35.0	41.0	32.0	41.0
18	37.03265094643564	38.0	35.0	41.0	32.0	41.0
19	36.99673633980624	38.0	35.0	41.0	32.0	41.0
20	36.87742511669966	38.0	35.0	41.0	31.0	41.0
21	36.764073458748236	38.0	35.0	41.0	31.0	41.0
22	36.62833233447812	38.0	35.0	41.0	31.0	41.0
23	36.51853753826036	38.0	35.0	40.0	31.0	41.0
24	36.42174115238533	38.0	35.0	40.0	31.0	41.0
25	36.30596183158584	38.0	35.0	40.0	30.0	41.0
26	36.13829415803104	38.0	35.0	40.0	30.0	41.0
27	36.008943507637156	38.0	35.0	40.0	30.0	41.0
28	35.91559984244003	38.0	35.0	40.0	30.0	41.0
29	36.04917351305651	38.0	35.0	40.0	30.0	41.0
30	36.02267004470033	38.0	35.0	40.0	30.0	41.0
31	35.93203576829275	38.0	35.0	40.0	30.0	41.0
32	35.79946506497914	38.0	34.0	40.0	29.0	41.0
33	35.59474928255993	38.0	34.0	40.0	29.0	41.0
34	35.444068650472495	38.0	34.0	40.0	27.0	41.0
35	35.30346445447731	38.0	34.0	40.0	27.0	41.0
36	35.171487613382496	37.0	34.0	40.0	26.0	41.0
37	35.06552538015072	37.0	34.0	40.0	26.0	41.0
38	34.998057181185644	37.0	34.0	40.0	26.0	41.0
39	34.91811385916629	37.0	34.0	40.0	26.0	41.0
40	34.79012564919612	37.0	33.0	40.0	25.0	41.0
41	34.706534808215494	37.0	33.0	40.0	25.0	41.0
42	34.62094721310789	36.0	33.0	40.0	25.0	41.0
43	34.503671474273	36.0	33.0	40.0	24.0	41.0
44	34.39033301325977	36.0	33.0	40.0	24.0	41.0
45	34.354637877450365	36.0	33.0	40.0	24.0	41.0
46	34.354543203763306	36.0	33.0	40.0	24.0	41.0
47	34.32512407273578	36.0	33.0	40.0	24.0	41.0
48	34.22913097875092	36.0	33.0	40.0	24.0	41.0
49	34.18466963330012	36.0	33.0	40.0	24.0	41.0
50	34.065659070872435	35.0	33.0	40.0	24.0	41.0
51	33.80668924108138	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	5.0
11	7.0
12	16.0
13	21.0
14	46.0
15	105.0
16	280.0
17	746.0
18	1774.0
19	3350.0
20	5801.0
21	9256.0
22	13617.0
23	19926.0
24	28092.0
25	39959.0
26	51815.0
27	62451.0
28	71102.0
29	81844.0
30	96398.0
31	117110.0
32	144816.0
33	185056.0
34	300417.0
35	412923.0
36	273241.0
37	342926.0
38	476746.0
39	745411.0
40	396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.07376485982413	24.42999985368612	28.54236661840221	13.953868668087537
2	31.390925727918724	25.79028860269384	29.362843217218447	13.45594245216899
3	28.98638621069141	25.64067548815044	31.25915716893544	14.113781132222705
4	26.8103832362163	27.678741769485637	30.138622360146165	15.372252634151899
5	24.044563191386874	32.113113826173944	29.212685011749578	14.629637970689599
6	22.481557996096573	41.42823002951811	25.56837921803551	10.521832756349808
7	88.60834557158091	3.730372781946129	5.989487778057336	1.6717938684156244
8	89.6666539478784	2.818120084678441	5.647601011803514	1.8676249556396396
9	84.84592718101638	4.796369809192356	7.3765433604052255	2.981159649386041
10	46.01525623433402	26.219533362003205	14.255017059911518	13.510193343751265
11	37.510948438128025	23.900831321039334	22.06054697866141	16.527673262171234
12	33.04123153827241	22.381376021794456	27.417069436264097	17.16032300366904
13	22.875515290230794	31.08418298185966	27.928881126284082	18.11142060162546
14	20.28205873383411	33.21775493113637	28.543169910292377	17.957016424737144
15	19.260988674445016	23.755091220966378	39.38043817851269	17.60348192607592
16	21.404200126403715	20.474217629560222	37.81071975814029	20.310862485895772
17	21.721988135952564	20.958746084310647	28.402938097466272	28.916327682270516
18	24.29005493082079	23.488857337368536	30.483033758054795	21.738053973755882
19	29.455996387481616	24.379535909586057	25.071141538022818	21.093326164909513
20	30.624872154661233	23.412888875755705	24.86997429752841	21.09226467205465
21	26.350756830061016	25.910552874250104	26.263169325036856	21.475520970652017
22	25.43729919495808	23.99932064457289	25.690479585340725	24.872900575128305
23	23.665237285252104	27.57586302955225	25.38333519333658	23.375564491859066
24	23.529308821837606	24.103662523306223	32.31445320064481	20.052575454211357
25	22.19719266697785	24.940176270929697	30.307313657081004	22.55531740501145
26	21.308866592438672	29.513890781565717	26.361343069613564	22.815899556382053
27	21.95456982715167	27.67925817141503	28.11650716062998	22.249664840803327
28	20.23044722989095	26.899749458997256	32.487476535987334	20.38232677512446
29	21.035948172754807	24.368806225053124	31.468816352268743	23.126429249923326
30	22.31490361788323	26.952508522783507	28.998722479004673	21.733865380328588
31	26.792969015597347	25.9184136591753	24.61059708399306	22.678020241234293
32	27.822817907786106	26.09708872674506	25.518976766790306	20.561116598678527
33	26.198389571894193	26.283136866306695	25.583211429007502	21.935262132791607
34	22.209012533361715	26.128187598492907	28.351728239468198	23.311071628677176
35	23.092547545555973	25.14352387512598	28.899200351612336	22.86472822770571
36	27.766530097482338	25.450983846086977	25.280169563442417	21.502316492988268
37	24.041407401818365	28.917331797133222	26.567272683456807	20.473988117591606
38	23.35508054865984	28.403368432407433	25.424245701742887	22.81730531718984
39	23.359240453091054	28.448983936170425	25.80730117736771	22.38447443337081
40	24.582080221892173	25.363195518090276	25.88415899785894	24.17056526215861
41	20.730783321479997	25.071858762924755	26.662434083445387	27.534923832149865
42	23.13365887693482	26.42798760750125	24.93636063445141	25.50199288111251
43	23.356687132440168	25.58628115158778	26.49580839422812	24.561223321743935
44	22.393740979103796	27.54244034912213	27.34775682174121	22.71606185003286
45	21.450590233060797	29.098904453306794	25.064198800972097	24.386306512660312
46	22.93702449782064	28.555104532660557	26.29991992901195	22.207951040506853
47	23.463094618891073	25.954561794232767	27.22648843532224	23.355855151553925
48	23.711942970866037	23.3071125472185	28.8265311245484	24.154413357367062
49	22.217389720216303	24.263517609449238	29.192115001562115	24.326977668772344
50	21.350551703739065	27.90965949891226	27.68433612372072	23.055452673627954
51	21.265460141373634	29.129659057101716	25.63872463641718	23.966156165107467
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	987.0
1	2596.0
2	4205.0
3	13994.5
4	23784.0
5	17638.0
6	11492.0
7	11276.0
8	11060.0
9	11506.0
10	11952.0
11	11935.0
12	11918.0
13	12144.5
14	12371.0
15	12385.5
16	12400.0
17	11761.0
18	11122.0
19	10966.0
20	10810.0
21	11866.5
22	12923.0
23	12682.5
24	12442.0
25	15222.0
26	21854.0
27	25706.0
28	29510.5
29	33315.0
30	40881.0
31	48447.0
32	57016.0
33	65585.0
34	72796.0
35	80007.0
36	87840.5
37	95674.0
38	111699.0
39	127724.0
40	159507.5
41	191291.0
42	219728.0
43	248165.0
44	257263.5
45	266362.0
46	269150.5
47	271939.0
48	267499.5
49	263060.0
50	255225.5
51	247391.0
52	234324.5
53	221258.0
54	209253.0
55	197248.0
56	190521.5
57	183795.0
58	173873.5
59	163952.0
60	160265.0
61	156578.0
62	142313.0
63	128048.0
64	111055.0
65	94062.0
66	81381.0
67	68700.0
68	58672.5
69	48645.0
70	41507.5
71	34370.0
72	28969.0
73	23568.0
74	19294.5
75	12685.5
76	10350.0
77	7383.0
78	4416.0
79	3733.5
80	3051.0
81	2155.0
82	1259.0
83	934.5
84	610.0
85	453.0
86	296.0
87	231.5
88	167.0
89	121.0
90	75.0
91	47.0
92	19.0
93	20.5
94	22.0
95	14.5
96	7.0
97	6.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3485657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.287273313049663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.12098852093922	14.469071418810806
2	7.326531635748464	2.6796457291927394
3	2.441845845202386	1.339641070786524
4	1.166881041537284	0.8535629012163346
5	0.7566211315601584	0.6918268713634762
6	0.5119082106278133	0.561684321536701
7	0.42192604369772874	0.540111381529587
8	0.3346135461904135	0.4895335498746288
9	0.2794739300660827	0.4599734528691524
>10	4.247725363919241	19.860365335246474
>50	2.3113109513304226	30.960928696642164
>100	1.0749306538888004	25.880364366973623
>500	0.00413094719977105	0.4970779439846566
>1k	9.532955076394732E-4	0.3612444515759432
>5k	0.0	0.0
>10k+	1.5888258460657886E-4	0.35496850839727323
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12217	0.3504934650770285	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4359	0.1250553339011842	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02888981904989504	0.0
2	0.0	0.0	0.0	0.10603452950189877	0.0
3	0.0	0.0	0.0	0.19106871387517474	0.0
4	0.0	0.0	0.0	0.3187921244115528	0.0
5	0.0	0.0	0.0	0.5570829258300516	0.0
6	0.0	0.0	0.0	0.9509541529760387	0.0
7	0.0	2.8688996077353568E-5	0.0	1.1577157477055258	0.0
8	0.0	2.8688996077353568E-5	0.0	1.6339817715856724	0.0
9	0.0	2.8688996077353568E-5	0.0	1.8828301235606373	0.0
10	0.0	2.8688996077353568E-5	0.0	2.1954828028116364	0.0
11	0.0	2.8688996077353568E-5	0.0	2.5024263718432422	0.0
12	0.0	2.8688996077353568E-5	0.0	2.7544878913788704	0.0
13	0.0	2.8688996077353568E-5	0.0	2.881035053076077	0.0
14	0.0	2.8688996077353568E-5	0.0	2.939302404109182	0.0
15	0.0	2.8688996077353568E-5	0.0	3.009877334459472	0.0
16	0.0	2.8688996077353568E-5	0.0	3.148789453466018	0.0
17	0.0	2.8688996077353568E-5	0.0	3.3055748170287553	0.0
18	0.0	2.8688996077353568E-5	0.0	3.4946639901745926	0.0
19	0.0	2.8688996077353568E-5	0.0	3.6040551322175416	0.0
20	0.0	2.8688996077353568E-5	0.0	3.7241759587934213	0.0
21	0.0	2.8688996077353568E-5	0.0	3.871034929713394	0.0
22	0.0	2.8688996077353568E-5	0.0	4.038004886883591	0.0
23	0.0	2.8688996077353568E-5	0.0	4.217798825300367	0.0
24	0.0	2.8688996077353568E-5	0.0	4.365432399114428	0.0
25	0.0	2.8688996077353568E-5	0.0	4.490172154058762	0.0
26	0.0	2.8688996077353568E-5	0.0	4.60650603315243	0.0
27	0.0	2.8688996077353568E-5	0.0	4.7265694817361545	0.0
28	0.0	2.8688996077353568E-5	0.0	4.853202710421593	0.0
29	0.0	2.8688996077353568E-5	0.0	4.991541049506592	0.0
30	0.0	2.8688996077353568E-5	0.0	5.162584844119774	0.0
31	0.0	2.8688996077353568E-5	0.0	5.312972561557261	0.0
32	0.0	2.8688996077353568E-5	0.0	5.462470920116351	0.0
33	0.0	2.8688996077353568E-5	0.0	5.6117971446989765	0.0
34	0.0	2.8688996077353568E-5	0.0	5.767119369461769	0.0
35	0.0	2.8688996077353568E-5	0.0	5.946970685870698	0.0
36	0.0	2.8688996077353568E-5	0.0	6.111817657331172	0.0
37	0.0	2.8688996077353568E-5	0.0	6.285185260626619	0.0
38	0.0	2.8688996077353568E-5	0.0	6.452183906792895	0.0
39	0.0	2.8688996077353568E-5	0.0	6.618121060104308	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATA	20	7.0352625E-4	45.0	19
TAAGCGT	40	6.8212103E-9	45.0	32
CGAATAT	305	0.0	42.78688	14
TAATACG	60	3.6379788E-12	41.250004	1
CGTTTTT	9905	0.0	39.979805	1
GCTACGA	340	0.0	39.044117	10
CGTTAGG	645	0.0	38.02326	2
CGCAATA	30	1.1403977E-4	37.500004	45
CAATACG	30	1.1403977E-4	37.500004	1
GGCGATA	1585	0.0	37.334385	8
GGGCGAT	8485	0.0	37.28344	7
GCGATCG	145	0.0	37.24138	9
GCGATAA	345	0.0	37.173912	9
ATACCGG	140	0.0	36.964283	2
GCGATAC	195	0.0	36.923077	9
TTTACGG	320	0.0	36.5625	2
TGGGCGA	2240	0.0	36.361607	6
TTACGGG	835	0.0	36.107784	3
CGAGGGA	1795	0.0	36.100277	4
ATTCGAC	50	4.8838046E-8	36.000004	12
>>END_MODULE