FastQCFastQC Report
Thu 26 May 2016
SRR1545339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545339_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5227188
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180980.3462282206035061No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC95960.1835786277440184No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT207.0355873E-445.030
CGTTTTT208450.042.7332731
AATCGCG603.6379788E-1241.2499961
CAATACG451.9304935E-840.01
TATCGGC356.252545E-638.57142633
AGGGCGA59400.038.106066
GGCGATT35500.037.5211268
TATCGGA301.14047616E-437.49999619
AGTACGG8250.037.3636362
GCGATCG1750.037.2857139
GGGCGAT122200.036.9353527
TATGGGC30550.036.824884
TGGGCGA38100.036.614176
TAGGGCG24950.036.6132245
TATAGCG3700.036.4864851
CGTTAGG10850.036.290322
TACGGGT7400.036.182434
TCGTTAG2250.036.01
CGAGGGA20450.035.9779934
GTTTTTT262150.035.8334922
Produced by FastQC (version 0.11.5)