##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545339_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5227188
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14034486611157	31.0	31.0	33.0	30.0	34.0
2	31.494189610168984	31.0	31.0	34.0	30.0	34.0
3	31.62373306642118	31.0	31.0	34.0	30.0	34.0
4	35.43664126868978	37.0	35.0	37.0	33.0	37.0
5	35.36055064405566	37.0	35.0	37.0	33.0	37.0
6	35.41984657907847	37.0	35.0	37.0	33.0	37.0
7	35.86122079404835	37.0	35.0	37.0	35.0	37.0
8	35.92109772979277	37.0	35.0	37.0	35.0	37.0
9	37.80128837914381	39.0	37.0	39.0	35.0	39.0
10	37.018487569224604	39.0	37.0	39.0	33.0	39.0
11	36.70940322024002	39.0	35.0	39.0	32.0	39.0
12	36.14267422560658	38.0	35.0	39.0	32.0	39.0
13	35.92151918010219	38.0	35.0	39.0	31.0	39.0
14	36.93166497933497	39.0	35.0	41.0	31.0	41.0
15	37.13272642958317	39.0	35.0	41.0	32.0	41.0
16	37.19946116344008	39.0	35.0	41.0	32.0	41.0
17	37.124887989488805	39.0	35.0	41.0	32.0	41.0
18	37.03665278539819	39.0	35.0	41.0	32.0	41.0
19	36.98389153020706	38.0	35.0	41.0	32.0	41.0
20	36.85216831688472	38.0	35.0	41.0	31.0	41.0
21	36.74121975333583	38.0	35.0	41.0	31.0	41.0
22	36.63452357175598	38.0	35.0	41.0	31.0	41.0
23	36.559828152345005	38.0	35.0	40.0	31.0	41.0
24	36.46607946758372	38.0	35.0	40.0	31.0	41.0
25	36.36083148339031	38.0	35.0	40.0	30.0	41.0
26	36.214297247391904	38.0	35.0	40.0	30.0	41.0
27	36.079553672069956	38.0	35.0	40.0	30.0	41.0
28	35.98433670264012	38.0	35.0	40.0	30.0	41.0
29	36.103597766141185	38.0	35.0	40.0	30.0	41.0
30	36.06562323758013	38.0	35.0	40.0	30.0	41.0
31	35.9494722592721	38.0	35.0	40.0	30.0	41.0
32	35.782014153690284	38.0	34.0	40.0	29.0	41.0
33	35.56495691373641	38.0	34.0	40.0	28.0	41.0
34	35.37294679280715	38.0	34.0	40.0	27.0	41.0
35	35.230565267597036	38.0	34.0	40.0	26.0	41.0
36	35.08211432227041	38.0	34.0	40.0	25.0	41.0
37	34.99173283991316	38.0	34.0	40.0	25.0	41.0
38	34.933632767751995	38.0	34.0	40.0	25.0	41.0
39	34.87891118513434	37.0	34.0	40.0	25.0	41.0
40	34.77521240866026	37.0	34.0	40.0	24.0	41.0
41	34.73433555479543	37.0	34.0	40.0	24.0	41.0
42	34.68177746811479	37.0	33.0	40.0	24.0	41.0
43	34.568876803359665	37.0	33.0	40.0	24.0	41.0
44	34.451337698204085	37.0	33.0	40.0	23.0	41.0
45	34.42061104364335	37.0	33.0	40.0	23.0	41.0
46	34.424501663226955	37.0	33.0	40.0	23.0	41.0
47	34.41240089317622	37.0	33.0	40.0	23.0	41.0
48	34.32807390895449	36.0	33.0	40.0	23.0	41.0
49	34.30179285688596	36.0	33.0	40.0	24.0	41.0
50	34.17896773561617	36.0	33.0	40.0	24.0	41.0
51	33.95105418056515	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	4.0
12	2.0
13	9.0
14	53.0
15	139.0
16	408.0
17	1134.0
18	2503.0
19	4988.0
20	8916.0
21	14254.0
22	21339.0
23	31534.0
24	46734.0
25	67983.0
26	89004.0
27	100063.0
28	107496.0
29	120294.0
30	142544.0
31	171546.0
32	211763.0
33	267904.0
34	430537.0
35	593446.0
36	388089.0
37	491042.0
38	707064.0
39	1205928.0
40	463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.532790479316986	23.764976503619152	28.35237607677398	14.349856940289884
2	31.484633803107904	25.110499182351965	30.025417107630336	13.379449906909796
3	28.79140754072744	25.046200748853874	32.182963382989094	13.979428327429586
4	24.859369894482462	27.829609342537516	32.38833192913666	14.92268883384336
5	22.38283757921085	32.4960571534829	31.298740355234976	13.822364912071269
6	20.397219307972087	41.251931248694326	28.04421803845586	10.306631404877727
7	88.44751709714669	3.3785851972418053	6.693292837372598	1.480604868238908
8	89.59034953401331	2.4913012503089615	6.199834404272431	1.718514811405291
9	84.75097892021485	4.251712392973047	8.405609287440972	2.591699399371134
10	43.344088638097574	22.450981292427212	19.565376259663896	14.639553809811318
11	37.6002355377308	25.733147535539185	20.386219129673545	16.280397797056466
12	30.812704651143214	23.657480848211314	27.806690710186814	17.723123790458654
13	21.519314017402856	33.72997106666146	28.617298631692606	16.133416284243076
14	19.268983629439003	35.50553375926024	29.41260195730477	15.812880653995991
15	18.148094156934857	25.62974585953289	40.353647123462935	15.868512860069314
16	19.317059191289847	22.397223899350855	39.24502045841856	19.040696450940736
17	19.98194822914347	23.897648219272007	29.03704630482011	27.083357246764418
18	23.05011796017285	25.43843841086259	32.00286655081088	19.508577078153685
19	27.14233733318947	27.18714153766805	26.032218470045464	19.63830265909701
20	28.049861608191634	25.47842166763468	26.596996320009918	19.874720404163767
21	23.884333220844553	27.878431003438177	28.192576964899672	20.044658810817594
22	23.318044042035606	25.95911989390854	27.200666974289046	23.522169089766813
23	20.869691313953123	30.080609306571716	26.589611852491245	22.460087526983916
24	20.89613000335936	26.800796145078387	33.791476411408965	18.51159744015329
25	19.775489230538483	27.5109485252874	31.965714644279103	20.74784759989501
26	19.79959396907094	31.381346911570812	28.269597343734336	20.54946177562391
27	20.782971647470877	29.886413115426496	29.24765667506124	20.08295856204139
28	19.187314479601653	28.34357593413514	33.53619575190332	18.932913834359887
29	19.659116909512342	26.095847327473205	32.85097073225604	21.394065030758412
30	20.85452063327357	28.97052870491744	29.405217489786097	20.769733172022892
31	25.08270221006017	27.45206409258668	26.622593256642002	20.842640440711143
32	25.611667305633546	27.14511129119519	27.277056038543098	19.96616536462817
33	25.01580199526017	28.206312839714204	26.117216369489675	20.660668795535955
34	20.310786602662848	28.400662076818357	28.491207892274012	22.797343428244783
35	21.355650495065415	27.49420912352875	29.240654822439904	21.90948555896593
36	26.22115370635225	27.408025117902778	25.879459472282228	20.49136170346274
37	21.313390679654145	30.701574154210636	27.64516983127448	20.339865334860733
38	21.727762613474013	31.053905082426724	25.44544026348392	21.772892040615336
39	21.37172032075372	30.180070049135406	26.483436218479227	21.964773411631647
40	22.41729205071637	27.188805912471487	26.338520826111477	24.05538121070067
41	19.184368344892132	25.676023131366232	27.410014715368952	27.729593808372687
42	21.563869522198168	27.91280895196423	25.968053951761444	24.555267574076154
43	21.886719972574163	28.220718290599073	26.544195464176916	23.348366272649844
44	20.855534562751522	30.064845572801286	27.28579878894733	21.793821075499867
45	19.884457953301087	32.04239066970616	25.503884689052697	22.569266687940054
46	22.17008456554461	30.774060546511816	26.037900301270973	21.017954586672605
47	22.142804123364225	27.526272251925892	26.992754039074164	23.33816958563572
48	22.576842462907397	25.840987544354633	28.9067850630205	22.67538492971747
49	21.667194675224994	26.02485313327166	29.023922613841325	23.284029577662025
50	20.20371182364208	29.90202380323799	27.336954400721762	22.557309972398162
51	19.97389418555445	30.541277642969796	25.492176673194077	23.992651498281674
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1202.0
1	2513.5
2	3825.0
3	19640.0
4	35455.0
5	27571.0
6	19687.0
7	20095.5
8	20504.0
9	21239.0
10	21974.0
11	22515.5
12	23057.0
13	23094.0
14	23131.0
15	22947.5
16	22764.0
17	21985.5
18	21207.0
19	21600.0
20	21993.0
21	21883.5
22	21774.0
23	23320.5
24	24867.0
25	29509.5
26	39694.0
27	45236.0
28	56881.0
29	68526.0
30	78466.5
31	88407.0
32	107572.5
33	126738.0
34	141259.5
35	155781.0
36	171856.5
37	187932.0
38	218549.0
39	249166.0
40	292069.5
41	334973.0
42	377043.0
43	419113.0
44	428803.0
45	438493.0
46	434002.5
47	429512.0
48	431057.0
49	432602.0
50	422196.0
51	411790.0
52	388571.5
53	365353.0
54	322031.5
55	278710.0
56	252758.5
57	226807.0
58	204634.5
59	182462.0
60	164817.5
61	147173.0
62	127175.0
63	107177.0
64	91138.5
65	75100.0
66	64975.0
67	54850.0
68	45061.5
69	35273.0
70	30672.0
71	26071.0
72	22490.5
73	18910.0
74	14679.5
75	8792.0
76	7135.0
77	5337.5
78	3540.0
79	2976.5
80	2413.0
81	1729.5
82	1046.0
83	701.0
84	356.0
85	343.0
86	330.0
87	226.0
88	122.0
89	74.5
90	27.0
91	18.0
92	9.0
93	9.0
94	9.0
95	6.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5227188.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.830673450382037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62021857703095	14.804716626217292
2	8.636579138621023	3.2526520297550854
3	2.8484978071634055	1.6091739609247018
4	1.4514046447577642	1.093237076392048
5	0.8714629847160911	0.8205117444641992
6	0.611733144801676	0.6911608253121378
7	0.4191565535495617	0.5525100129135558
8	0.315854737725554	0.4758205939092777
9	0.2848638233223907	0.48277598713301356
>10	3.2391843226816786	14.52165658830463
>50	1.2977565021060118	17.728175774075456
>100	1.3958662996741402	42.448843614637404
>500	0.006287629095018192	0.744000250189976
>1k	9.276829812263198E-4	0.2372275979696172
>5k	1.0307588680292443E-4	0.18625724350486392
>10k+	1.0307588680292443E-4	0.35128007429668895
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18098	0.3462282206035061	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9596	0.1835786277440184	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03699886057283572	0.0
2	0.0	0.0	0.0	0.11929932499079811	0.0
3	0.0	0.0	0.0	0.19345009209540578	0.0
4	0.0	0.0	0.0	0.3415794496008179	0.0
5	0.0	0.0	0.0	0.6058515591939682	0.0
6	0.0	0.0	0.0	1.0876976301598489	0.0
7	0.0	0.0	0.0	1.3579385321515125	0.0
8	0.0	0.0	0.0	2.0329286032949265	0.0
9	0.0	0.0	0.0	2.3703566812595986	0.0
10	0.0	0.0	0.0	2.8095794526617373	0.0
11	0.0	0.0	0.0	3.1762393087832312	0.0
12	0.0	0.0	0.0	3.477433755969749	0.0
13	0.0	0.0	0.0	3.6341145564307236	0.0
14	0.0	0.0	0.0	3.7033104606147704	0.0
15	0.0	0.0	0.0	3.7849030874726526	0.0
16	0.0	0.0	0.0	3.9488344402382314	0.0
17	0.0	0.0	0.0	4.142667147230978	0.0
18	0.0	0.0	0.0	4.387234589611087	0.0
19	0.0	0.0	0.0	4.528094264067028	0.0
20	0.0	0.0	0.0	4.676070575613504	0.0
21	0.0	0.0	0.0	4.873729431579656	0.0
22	0.0	0.0	0.0	5.086654621949698	0.0
23	0.0	0.0	0.0	5.334340375743134	0.0
24	0.0	0.0	0.0	5.523581703967793	0.0
25	0.0	0.0	0.0	5.694400124885503	0.0
26	0.0	0.0	0.0	5.851214840560546	0.0
27	0.0	0.0	0.0	6.0073217186755095	0.0
28	0.0	0.0	0.0	6.18606026796817	0.0
29	0.0	0.0	0.0	6.373771136603466	0.0
30	0.0	0.0	0.0	6.605635764391868	0.0
31	0.0	0.0	0.0	6.81695397219308	0.0
32	0.0	0.0	0.0	7.023680801226204	0.0
33	0.0	0.0	0.0	7.2323015740011645	0.0
34	0.0	0.0	0.0	7.446317216828628	0.0
35	0.0	0.0	0.0	7.682849746364584	0.0
36	1.9130744867029843E-5	0.0	0.0	7.903159404253301	0.0
37	1.9130744867029843E-5	0.0	0.0	8.12859610176638	0.0
38	1.9130744867029843E-5	0.0	0.0	8.360766821472653	0.0
39	1.9130744867029843E-5	0.0	0.0	8.608337790796888	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAT	20	7.0355873E-4	45.0	30
CGTTTTT	20845	0.0	42.733273	1
AATCGCG	60	3.6379788E-12	41.249996	1
CAATACG	45	1.9304935E-8	40.0	1
TATCGGC	35	6.252545E-6	38.571426	33
AGGGCGA	5940	0.0	38.10606	6
GGCGATT	3550	0.0	37.521126	8
TATCGGA	30	1.14047616E-4	37.499996	19
AGTACGG	825	0.0	37.363636	2
GCGATCG	175	0.0	37.285713	9
GGGCGAT	12220	0.0	36.935352	7
TATGGGC	3055	0.0	36.82488	4
TGGGCGA	3810	0.0	36.61417	6
TAGGGCG	2495	0.0	36.613224	5
TATAGCG	370	0.0	36.486485	1
CGTTAGG	1085	0.0	36.29032	2
TACGGGT	740	0.0	36.18243	4
TCGTTAG	225	0.0	36.0	1
CGAGGGA	2045	0.0	35.977993	4
GTTTTTT	26215	0.0	35.833492	2
>>END_MODULE