FastQCFastQC Report
Thu 26 May 2016
SRR1545338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545338_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5009674
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130280.2600568420220557No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC96790.19320618467389294No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT54700.10918874162270838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAA302.1669675E-645.00000439
TTATACG451.9304935E-840.0000041
TATCACG1350.040.01
CGTAAGG6550.039.847332
CGAATAT7000.039.53571314
TACGAAT7100.038.9788712
AATACGG6250.038.8799972
TGCCGAT2450.038.5714324
GCTACGA7150.038.3916110
CGTTTTT113100.038.2957531
TACGTAG1950.038.0769231
GGACTAA24600.037.8658528
CTACGAA7700.037.402611
GCGATAC4050.037.222229
GTTGATC18450.037.1951216
GGGCGAT110500.037.1606337
CATACGA8750.037.02857218
ATACTAT20300.036.90886745
TATCGAT1100.036.81818431
CTATGCG2400.036.5625041
Produced by FastQC (version 0.11.5)