##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545338_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 5009674 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.180605763967876 31.0 31.0 33.0 30.0 34.0 2 31.539707773400025 31.0 31.0 34.0 30.0 34.0 3 31.728625056241185 31.0 31.0 34.0 30.0 34.0 4 35.520816524189 37.0 35.0 37.0 33.0 37.0 5 35.428891380956124 37.0 35.0 37.0 33.0 37.0 6 35.47343799217274 37.0 35.0 37.0 33.0 37.0 7 35.8214540507027 37.0 35.0 37.0 35.0 37.0 8 35.86101291221744 37.0 35.0 37.0 35.0 37.0 9 37.7446199093993 39.0 37.0 39.0 35.0 39.0 10 37.112806741516515 39.0 37.0 39.0 33.0 39.0 11 36.737741617518424 39.0 35.0 39.0 32.0 39.0 12 35.98021128720152 37.0 35.0 39.0 32.0 39.0 13 35.6335076893227 37.0 35.0 39.0 30.0 39.0 14 36.57916922338659 38.0 35.0 41.0 31.0 41.0 15 36.84476375109438 38.0 35.0 41.0 31.0 41.0 16 36.958491510625244 38.0 35.0 41.0 32.0 41.0 17 36.88411940577371 38.0 35.0 41.0 32.0 41.0 18 36.84022692893789 38.0 35.0 41.0 31.0 41.0 19 36.80274285312777 38.0 35.0 41.0 31.0 41.0 20 36.66257644709017 38.0 35.0 40.0 31.0 41.0 21 36.52358536703186 38.0 35.0 40.0 31.0 41.0 22 36.41059238585186 38.0 35.0 40.0 31.0 41.0 23 36.31218019376111 38.0 35.0 40.0 31.0 41.0 24 36.201026054789196 38.0 35.0 40.0 30.0 41.0 25 36.05598747543253 38.0 35.0 40.0 30.0 41.0 26 35.88230052494434 37.0 34.0 40.0 30.0 41.0 27 35.73933533399578 37.0 34.0 40.0 30.0 41.0 28 35.64416267405823 37.0 34.0 40.0 29.0 41.0 29 35.80446212667731 37.0 34.0 40.0 30.0 41.0 30 35.79771398298572 37.0 34.0 40.0 30.0 41.0 31 35.685643017888985 37.0 34.0 40.0 29.0 41.0 32 35.568767947774646 37.0 34.0 40.0 29.0 41.0 33 35.38697448177267 37.0 34.0 40.0 28.0 41.0 34 35.2308265967007 37.0 34.0 40.0 27.0 41.0 35 35.10070156261665 37.0 34.0 40.0 27.0 41.0 36 34.95362931799554 37.0 34.0 40.0 26.0 41.0 37 34.810362311000674 36.0 33.0 40.0 26.0 41.0 38 34.75455648411454 36.0 33.0 40.0 26.0 41.0 39 34.684744356618815 36.0 33.0 40.0 26.0 41.0 40 34.5160888712519 36.0 33.0 40.0 24.0 41.0 41 34.478700011218294 36.0 33.0 40.0 25.0 41.0 42 34.4250749649578 36.0 33.0 40.0 25.0 41.0 43 34.28608148953405 35.0 33.0 40.0 24.0 41.0 44 34.16783906497708 35.0 33.0 40.0 24.0 41.0 45 34.125569647845346 35.0 33.0 40.0 24.0 41.0 46 34.128791613985264 35.0 33.0 40.0 24.0 41.0 47 34.107545121698536 35.0 33.0 40.0 24.0 41.0 48 34.01760653487632 35.0 33.0 39.0 24.0 41.0 49 33.9733854937467 35.0 33.0 39.0 24.0 41.0 50 33.83520404720946 35.0 33.0 39.0 24.0 41.0 51 33.58118053190687 35.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 3.0 11 12.0 12 12.0 13 29.0 14 81.0 15 214.0 16 445.0 17 1104.0 18 2665.0 19 5015.0 20 8789.0 21 13913.0 22 20558.0 23 29600.0 24 41937.0 25 57461.0 26 75383.0 27 91319.0 28 104603.0 29 120641.0 30 144923.0 31 176100.0 32 217797.0 33 282449.0 34 481746.0 35 667472.0 36 382796.0 37 478070.0 38 646000.0 39 957991.0 40 541.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.96987508568422 23.984135494644963 28.71234735034655 13.333642069324272 2 30.361277001257964 25.80475296396532 30.119923971100715 13.714046063676 3 29.396323992339624 26.453238274586333 30.051735901378013 14.098701831696033 4 26.8914104989666 28.306692211908402 29.42357127429849 15.378326014826513 5 23.75230404213927 32.65998945240749 28.614436787703152 14.973269717750098 6 23.020479975343704 40.56092272670836 25.605957593248586 10.812639704699349 7 88.34379243040566 4.337907017502536 5.4495961214242685 1.8687044306675444 8 89.51510617257729 3.1716834269056227 5.04475939951382 2.2684510010032586 9 84.43916709949589 4.905269284987406 7.461044371350312 3.1945192441663868 10 52.00516041562784 21.849325924201853 12.802609511117888 13.342904149052416 11 42.65818494377079 21.73265166555748 19.12080506635761 16.488358324314117 12 37.25461976168509 21.195970037172078 24.534590474350225 17.014819726792602 13 23.58522730221567 32.88064253282749 25.87312068609654 17.6610094788603 14 19.075333045623328 35.41448006397223 27.816420789057332 17.693766101347112 15 18.59156903223643 22.673092101402208 41.262086115783184 17.47325275057818 16 20.52890068295861 18.363270743764964 39.75392410763654 21.353904465639882 17 20.36952903522265 19.86031426396209 27.415276922210907 32.35487977860436 18 24.25079955302481 22.969498614081473 30.390420614195655 22.389281218698063 19 30.464796711322933 23.874667293720112 23.829255157122002 21.831280837834957 20 31.6021561482843 22.763277610479246 23.536781036051448 22.09778520518501 21 25.873939102624245 25.469681260696802 25.744409716081325 22.911969920597627 22 24.841157328800236 23.45539849499189 24.314755810457925 27.388688365749946 23 23.930259733467686 27.500891275560047 24.001142589318185 24.56770640165408 24 23.826540409615475 23.09317931665813 32.75562441787629 20.3246558558501 25 22.233283043966534 23.554287165192786 30.533503776892466 23.678926013948214 26 21.238028662144483 29.11562708471649 25.45520926112158 24.191134992017442 27 22.22406088699584 27.64640972646124 27.10288134517336 23.02664804136956 28 19.952974984000953 26.715810250327664 32.94621566193728 20.384999103734096 29 21.072688562169915 24.193889662281418 31.128712167697937 23.60470960785073 30 23.00882253016863 26.814120040545554 28.16380866299883 22.01324876628699 31 28.529002086762535 25.753951255111613 22.99071755966556 22.726329098460297 32 30.1168898415346 25.771317654601877 23.4478131710766 20.663979332786926 33 27.985653357883166 25.7606383169843 23.414377861713156 22.839330463419376 34 22.67019770148716 25.364285181031736 27.337267854155776 24.62824926332532 35 22.66143465622713 25.087380935366255 28.108715257719364 24.14246915068725 36 30.04271335819457 24.584453998403887 23.838936425803357 21.53389621759819 37 24.207363592920416 28.982524611381898 25.88583608434401 20.924275711353673 38 23.755917051688392 29.481858500173864 22.850329183096544 23.911895265041196 39 23.863369153362076 28.46859895474236 25.350451945575703 22.31757994631986 40 24.571279488445754 24.763467642804702 25.55268067343304 25.112572195316503 41 20.34525599869373 23.486598129938198 26.111659161853645 30.05648670951443 42 23.77867302343426 24.947431709129177 24.006612007088684 27.26728326034788 43 23.528297450093557 25.12375056740219 25.876793579781836 25.47115840272241 44 22.89342579976262 27.872831645332614 25.881205044479938 23.35253751042483 45 21.36162951920624 29.982809260642508 23.37277834845142 25.282782871699833 46 23.367608351361785 28.82536867668435 25.286535610900028 22.520487361053835 47 24.111269515740943 25.01747618707325 26.793879202518966 24.077375094666838 48 24.46803923768293 22.165973274907707 28.508082561859315 24.857904925550045 49 22.513880144696042 23.500291635743164 29.135149313109 24.850678906451797 50 21.252800082400572 28.605893317609087 26.442698666619822 23.698607933370514 51 21.085064616979068 29.891485952978176 23.93534988504242 25.088099545000333 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1450.0 1 3700.5 2 5951.0 3 15898.0 4 25845.0 5 19466.5 6 13088.0 7 13142.5 8 13197.0 9 13711.5 10 14226.0 11 14468.5 12 14711.0 13 14805.0 14 14899.0 15 14590.0 16 14281.0 17 13454.5 18 12628.0 19 12468.5 20 12309.0 21 12568.5 22 12828.0 23 13342.0 24 13856.0 25 16632.0 26 23573.5 27 27739.0 28 34036.0 29 40333.0 30 47378.0 31 54423.0 32 62953.5 33 71484.0 34 85021.5 35 98559.0 36 99131.0 37 99703.0 38 126690.0 39 153677.0 40 205932.0 41 258187.0 42 313389.5 43 368592.0 44 387714.0 45 406836.0 46 405951.0 47 405066.0 48 394749.0 49 384432.0 50 369779.5 51 355127.0 52 341556.0 53 327985.0 54 302958.0 55 277931.0 56 273992.5 57 270054.0 58 266754.5 59 263455.0 60 256585.0 61 249715.0 62 223468.0 63 197221.0 64 172601.5 65 147982.0 66 132653.0 67 117324.0 68 102250.0 69 87176.0 70 72935.0 71 58694.0 72 49982.5 73 41271.0 74 34190.0 75 21551.5 76 15994.0 77 11940.0 78 7886.0 79 5652.0 80 3418.0 81 2791.5 82 2165.0 83 1445.0 84 725.0 85 577.0 86 429.0 87 299.5 88 170.0 89 126.0 90 82.0 91 53.5 92 25.0 93 21.0 94 17.0 95 13.5 96 10.0 97 5.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 5009674.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.848126028332837 #Duplication Level Percentage of deduplicated Percentage of total 1 79.64166570249876 12.62171155159553 2 8.822951500607825 2.796544946470023 3 2.8485250001042903 1.3543134958952878 4 1.3345390398236612 0.8459977157142272 5 0.7824647053490696 0.6200299631547188 6 0.4817200266301531 0.458061581544391 7 0.3646600048499825 0.40454244000484896 8 0.27482979284906184 0.34844297547300307 9 0.2215590524053147 0.31601662067136843 >10 2.156364759026375 7.8762181627030206 >50 1.022104819736867 12.023614946808234 >100 2.032975094282875 57.757234667284905 >500 0.011952903842030998 1.2077443448781957 >1k 0.0033061223392851697 0.8016967100155912 >5k 2.5431710302193614E-4 0.3052863973663011 >10k+ 1.2715855151096807E-4 0.262543480420369 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13028 0.2600568420220557 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 9679 0.19320618467389294 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 5470 0.10918874162270838 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03986287331271456 0.0 2 0.0 0.0 0.0 0.12711405971725906 0.0 3 0.0 0.0 0.0 0.19759768799327063 0.0 4 0.0 0.0 0.0 0.37836793372183497 0.0 5 0.0 0.0 0.0 0.655252218008597 0.0 6 0.0 0.0 0.0 1.1141243921261144 0.0 7 0.0 0.0 0.0 1.3487903604106775 0.0 8 0.0 0.0 0.0 1.9543387453954089 0.0 9 0.0 0.0 0.0 2.2432996638104594 0.0 10 1.9961378724443947E-5 0.0 0.0 2.6266180194559565 0.0 11 1.9961378724443947E-5 0.0 0.0 2.9828088614149344 0.0 12 1.9961378724443947E-5 0.0 0.0 3.2776384251749713 0.0 13 1.9961378724443947E-5 0.0 0.0 3.410800782645737 0.0 14 1.9961378724443947E-5 0.0 0.0 3.463977895567656 0.0 15 1.9961378724443947E-5 0.0 0.0 3.534281871435147 0.0 16 1.9961378724443947E-5 0.0 0.0 3.6803792023193527 0.0 17 1.9961378724443947E-5 0.0 0.0 3.8425254816980106 0.0 18 1.9961378724443947E-5 0.0 0.0 4.054615130645228 0.0 19 1.9961378724443947E-5 0.0 0.0 4.165640319110585 0.0 20 1.9961378724443947E-5 0.0 0.0 4.291516773346928 0.0 21 3.9922757448887894E-5 0.0 0.0 4.456258031959765 0.0 22 3.9922757448887894E-5 0.0 0.0 4.627347009006973 0.0 23 3.9922757448887894E-5 0.0 0.0 4.818317519263728 0.0 24 3.9922757448887894E-5 0.0 0.0 4.969724576888636 0.0 25 3.9922757448887894E-5 0.0 0.0 5.0977368986484946 0.0 26 3.9922757448887894E-5 0.0 0.0 5.214890230382257 0.0 27 3.9922757448887894E-5 0.0 0.0 5.334079622745911 0.0 28 3.9922757448887894E-5 0.0 0.0 5.459836308709908 0.0 29 3.9922757448887894E-5 0.0 0.0 5.590842837278434 0.0 30 3.9922757448887894E-5 0.0 0.0 5.757680040657336 0.0 31 3.9922757448887894E-5 0.0 0.0 5.913797983661213 0.0 32 3.9922757448887894E-5 0.0 0.0 6.062250757234902 0.0 33 3.9922757448887894E-5 0.0 0.0 6.215194841021591 0.0 34 3.9922757448887894E-5 0.0 0.0 6.371552320570161 0.0 35 3.9922757448887894E-5 0.0 0.0 6.560426886060849 0.0 36 3.9922757448887894E-5 0.0 0.0 6.719539035873392 0.0 37 3.9922757448887894E-5 0.0 0.0 6.87970913875833 0.0 38 3.9922757448887894E-5 0.0 0.0 7.0465463421372325 0.0 39 3.9922757448887894E-5 0.0 0.0 7.233644344921446 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTAA 30 2.1669675E-6 45.000004 39 TTATACG 45 1.9304935E-8 40.000004 1 TATCACG 135 0.0 40.0 1 CGTAAGG 655 0.0 39.84733 2 CGAATAT 700 0.0 39.535713 14 TACGAAT 710 0.0 38.97887 12 AATACGG 625 0.0 38.879997 2 TGCCGAT 245 0.0 38.57143 24 GCTACGA 715 0.0 38.39161 10 CGTTTTT 11310 0.0 38.295753 1 TACGTAG 195 0.0 38.076923 1 GGACTAA 2460 0.0 37.865852 8 CTACGAA 770 0.0 37.4026 11 GCGATAC 405 0.0 37.22222 9 GTTGATC 1845 0.0 37.19512 16 GGGCGAT 11050 0.0 37.160633 7 CATACGA 875 0.0 37.028572 18 ATACTAT 2030 0.0 36.908867 45 TATCGAT 110 0.0 36.818184 31 CTATGCG 240 0.0 36.562504 1 >>END_MODULE