FastQCFastQC Report
Thu 26 May 2016
SRR1545336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545336_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences598159
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24870.41577573855780825No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8960.14979294802886858No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6640.1110072739856794No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACTA502.1827873E-1145.08
CCGTAGG207.0305704E-445.02
GTTGATC1000.042.7516
CGTTTTT24200.042.3037221
GCGATAT750.042.09
TACGAAT1200.041.24999612
CGAATAT1150.041.0869614
CGAATGG501.0804797E-940.52
TATAGCG451.9255822E-840.01
TGCGTAG451.9255822E-840.01
CTTACGG451.9255822E-840.02
GGATGCC3300.039.5454568
CGAGGGT800.039.3754
GTCCACG403.4545155E-739.37540
AACGGGA1550.039.1935464
CACGTGA2300.039.13043643
CGTTAGG1100.038.8636362
AGGGCGA7150.038.706296
ACGACCA2500.038.728
TGTACGG700.038.5714262