##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545336_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	598159
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13275901557947	31.0	31.0	33.0	30.0	34.0
2	31.48584573666868	31.0	31.0	34.0	30.0	34.0
3	31.603023610779072	31.0	31.0	34.0	30.0	34.0
4	35.424403879236124	37.0	35.0	37.0	33.0	37.0
5	35.35447431201403	37.0	35.0	37.0	33.0	37.0
6	35.41373614707795	37.0	35.0	37.0	33.0	37.0
7	35.83278191918871	37.0	35.0	37.0	35.0	37.0
8	35.89196852341936	37.0	35.0	37.0	35.0	37.0
9	37.78928177959372	39.0	37.0	39.0	35.0	39.0
10	37.06543912237382	39.0	37.0	39.0	33.0	39.0
11	36.70758109465878	39.0	35.0	39.0	32.0	39.0
12	36.05669897134374	37.0	35.0	39.0	32.0	39.0
13	35.7854684122449	38.0	35.0	39.0	31.0	39.0
14	36.776121399159756	39.0	35.0	41.0	31.0	41.0
15	37.013063416248855	39.0	35.0	41.0	32.0	41.0
16	37.07867640543735	39.0	35.0	41.0	32.0	41.0
17	36.987377937973015	38.0	35.0	41.0	32.0	41.0
18	36.90841398357293	38.0	35.0	40.0	32.0	41.0
19	36.86525321862582	38.0	35.0	40.0	31.0	41.0
20	36.72232800977667	38.0	35.0	40.0	31.0	41.0
21	36.60901700049652	38.0	35.0	40.0	31.0	41.0
22	36.47853664326709	38.0	35.0	40.0	31.0	41.0
23	36.401990106309526	38.0	35.0	40.0	31.0	41.0
24	36.31627376667408	38.0	35.0	40.0	31.0	41.0
25	36.197449173213144	38.0	35.0	40.0	30.0	41.0
26	36.02534610362797	38.0	35.0	40.0	30.0	41.0
27	35.89321902704799	38.0	34.0	40.0	30.0	41.0
28	35.83656519420422	37.0	34.0	40.0	30.0	41.0
29	35.96731638243343	38.0	35.0	40.0	30.0	41.0
30	35.92329297059812	38.0	35.0	40.0	30.0	41.0
31	35.79261534140588	38.0	34.0	40.0	30.0	41.0
32	35.61874351134063	38.0	34.0	40.0	29.0	41.0
33	35.35514470232831	37.0	34.0	40.0	27.0	41.0
34	35.17029920138291	37.0	34.0	40.0	27.0	41.0
35	35.01976899118796	37.0	34.0	40.0	26.0	41.0
36	34.864898797811286	37.0	34.0	40.0	25.0	41.0
37	34.7661106829455	37.0	34.0	40.0	24.0	41.0
38	34.72540913034828	37.0	33.0	40.0	25.0	41.0
39	34.64928221426076	37.0	33.0	40.0	24.0	41.0
40	34.54216019486458	37.0	33.0	40.0	24.0	41.0
41	34.4839114683554	37.0	33.0	40.0	23.0	41.0
42	34.40637857158381	36.0	33.0	40.0	23.0	41.0
43	34.28467180131035	36.0	33.0	40.0	23.0	41.0
44	34.157488226374596	36.0	33.0	40.0	23.0	41.0
45	34.10966314976453	36.0	33.0	40.0	23.0	41.0
46	34.11647237607392	36.0	33.0	40.0	23.0	41.0
47	34.112348054614245	36.0	33.0	40.0	23.0	41.0
48	34.03578479969373	36.0	33.0	40.0	23.0	41.0
49	34.003485695275	36.0	33.0	40.0	23.0	41.0
50	33.861949749146966	35.0	33.0	40.0	23.0	41.0
51	33.61669723267559	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	2.0
14	8.0
15	16.0
16	64.0
17	135.0
18	328.0
19	604.0
20	1030.0
21	1757.0
22	2614.0
23	3801.0
24	5626.0
25	7907.0
26	10460.0
27	11976.0
28	12888.0
29	14688.0
30	16864.0
31	20093.0
32	24984.0
33	31761.0
34	51521.0
35	73120.0
36	44604.0
37	55581.0
38	79780.0
39	125890.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.575855249189594	23.89715777911893	27.85212627411775	14.674860697573722
2	31.748581898792793	25.265021507659334	29.54465284314037	13.4417437504075
3	28.179965527560398	25.127432672583712	32.46962764081122	14.222974159044668
4	24.85710321168786	28.3234056496684	31.909074343109438	14.910416795534298
5	23.02949550203207	32.44154146305581	30.511118281259662	14.017844753652456
6	20.874717257451614	41.27397564861517	27.23155548942672	10.619751604506494
7	88.64933905533479	3.3330602732718226	6.5780168817989875	1.4395837895944055
8	89.95634939873847	2.3722789425554076	6.147863695104479	1.523507963601651
9	85.29187055615647	4.267260042898293	8.002220145479713	2.43864925546552
10	46.351388176053526	26.5295682251709	15.107521578710678	12.011522020064898
11	38.73351399878628	23.516991301643877	22.254785098945266	15.494709600624585
12	33.34447864196643	23.437580977633036	26.95102807113159	16.266912309268942
13	21.71880720677947	35.01008929064011	27.274687833836825	15.996415668743595
14	18.9763925645188	35.82910229554349	28.94330771584144	16.25119742409627
15	18.30483199283134	24.523914210101328	40.940117928510645	16.231135868556688
16	20.05687450995471	21.667650240153538	39.11368047626133	19.161794773630422
17	19.883509234166834	21.900865823301164	28.721125988240587	29.494498954291416
18	23.458478431320103	23.964363990176523	31.53960736192216	21.03755021658121
19	27.57109731693413	26.02100779224253	25.38806571496876	21.01982917585458
20	30.08263689086012	24.01000402902907	25.51729556856956	20.390063511541246
21	24.669026128504292	27.479984418858532	27.248607811635367	20.602381641001806
22	24.55785167488912	24.62823429890715	26.182168955077163	24.631745071126574
23	21.670492293854977	29.260280293366815	25.410802144580284	23.65842526819792
24	22.04932133429406	25.466138601943634	33.72932614906739	18.75521391469492
25	20.472483068883022	26.30337418646213	31.539273002663172	21.684869741991676
26	20.897119327804145	30.6483727570763	27.321832489354836	21.13267542576472
27	20.742478170519878	29.747441733719633	28.115099831315753	21.394980264444737
28	18.560784005590484	27.679262537218364	33.941644278527946	19.8183091786632
29	20.036812954415133	24.209114967759408	33.239489834642626	22.514582243182833
30	22.12003831757108	27.344736098595856	29.78321817443188	20.75200740940118
31	27.29207451530446	26.32995574755207	24.89739350239652	21.480576234746948
32	28.135161386855334	26.441297380796748	25.629473099961714	19.794068132386204
33	26.107606840321722	26.514522058516217	25.807352225746	21.570518875416067
34	20.73729560200549	26.73870994167103	28.87158765478744	23.652406801536046
35	21.31306224599145	26.612990860289653	29.89338955027008	22.180557343448815
36	27.648668665020505	26.193871529141916	25.593696659249467	20.563763146588116
37	21.95737253807098	29.748611991126104	27.355268415254137	20.938747055548777
38	21.24134218493745	30.937426336475752	24.887529904256226	22.93370157433057
39	21.91591198995585	30.787131849558396	25.73513062580351	21.56182553468225
40	24.23486063070187	26.186515625444073	25.23492917434996	24.343694569504095
41	19.973619054465452	25.405118037177406	26.24970952539375	28.371553382963395
42	22.170693745308522	27.070895865480583	24.307750949162347	26.450659440048547
43	22.560222282035376	26.744561228703407	26.122318647717414	24.572897841543803
44	21.28815248119647	29.62540060418718	27.22369804684039	21.86274886777596
45	20.302461385685078	31.274962008429195	24.624890706317217	23.79768589956851
46	22.33101900999567	30.255834987018503	25.800832220195634	21.612313782790192
47	21.971582806578187	26.706444273178203	27.14963747097344	24.17233544927018
48	23.534879522000004	24.342858671356613	28.77830142152839	23.343960385114997
49	22.206804545279766	24.227170367745032	29.60299853383465	23.963026553140555
50	20.14447663581088	29.957920887255728	27.235902159793635	22.661700317139758
51	19.57807205107672	30.614769651547498	25.89044050160576	23.91671779577002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	137.0
1	300.0
2	463.0
3	2454.0
4	4445.0
5	3334.0
6	2223.0
7	2323.0
8	2423.0
9	2512.0
10	2601.0
11	2624.5
12	2648.0
13	2659.0
14	2670.0
15	2566.5
16	2463.0
17	2386.0
18	2309.0
19	2337.5
20	2366.0
21	2258.5
22	2151.0
23	2461.0
24	2771.0
25	2905.5
26	3452.5
27	3865.0
28	4713.0
29	5561.0
30	6943.5
31	8326.0
32	9661.0
33	10996.0
34	13237.0
35	15478.0
36	17076.5
37	18675.0
38	21156.0
39	23637.0
40	29522.5
41	35408.0
42	40747.0
43	46086.0
44	48608.0
45	51130.0
46	50265.0
47	49400.0
48	48629.5
49	47859.0
50	47237.0
51	46615.0
52	43586.0
53	40557.0
54	37573.0
55	34589.0
56	31866.0
57	29143.0
58	27373.5
59	25604.0
60	23413.5
61	21223.0
62	18721.5
63	16220.0
64	14330.5
65	12441.0
66	10532.0
67	8623.0
68	6983.5
69	5344.0
70	4398.5
71	3453.0
72	2649.5
73	1846.0
74	1674.0
75	1210.5
76	919.0
77	713.5
78	508.0
79	355.5
80	203.0
81	167.0
82	131.0
83	90.5
84	50.0
85	46.5
86	43.0
87	25.0
88	7.0
89	5.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	598159.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.90008562457435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.87438022021881	22.209245030179744
2	6.5614400087661755	4.054981161827654
3	3.301865979091761	3.0608382442441333
4	2.236381630374362	2.764175354711719
5	1.7517938115076408	2.7065289386092775
6	1.5338802171082713	2.8438218027891766
7	1.2475845622286466	2.6985328857733597
8	1.1363030441779027	2.808948908844935
9	0.9704545310002674	2.6988415292397963
>10	9.31128506974796	51.92608862338239
>50	0.06591490524930062	1.253711508909363
>100	0.007081766679674858	0.2930601150658783
>500	0.0010895025661038242	0.26259263613025285
>1k	5.447512830519121E-4	0.4186332602922685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2487	0.41577573855780825	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	896	0.14979294802886858	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	664	0.1110072739856794	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03477336293527306	0.0
2	0.0	0.0	0.0	0.10649342398927375	0.0
3	0.0	0.0	0.0	0.18239297578068708	0.0
4	0.0	0.0	0.0	0.30025461457572317	0.0
5	0.0	0.0	0.0	0.5510240588204809	0.0
6	0.0	0.0	0.0	0.9711464677451982	0.0
7	0.0	0.0	0.0	1.209377439777718	0.0
8	0.0	0.0	0.0	1.751206618975891	0.0
9	0.0	0.0	0.0	2.0165206909868445	0.0
10	0.0	0.0	0.0	2.339678914803589	0.0
11	0.0	0.0	0.0	2.6421068645627668	0.0
12	0.0	0.0	0.0	2.8688024421600278	0.0
13	0.0	0.0	0.0	2.989004595768015	0.0
14	0.0	0.0	0.0	3.0503595197932323	0.0
15	0.0	0.0	0.0	3.119070347516296	0.0
16	0.0	0.0	0.0	3.2600027751818494	0.0
17	0.0	0.0	0.0	3.4221670157934594	0.0
18	0.0	0.0	0.0	3.6259589841496993	0.0
19	0.0	0.0	0.0	3.736799078505882	0.0
20	0.0	0.0	0.0	3.8576699506318555	0.0
21	0.0	0.0	0.0	4.019499831984472	0.0
22	0.0	0.0	0.0	4.20523640035509	0.0
23	0.0	0.0	0.0	4.420563763146588	0.0
24	0.0	0.0	0.0	4.592424422268995	0.0
25	0.0	0.0	0.0	4.742718909186354	0.0
26	0.0	0.0	0.0	4.883316977592914	0.0
27	0.0	0.0	0.0	5.021072992298034	0.0
28	0.0	0.0	0.0	5.175546969952805	0.0
29	0.0	0.0	0.0	5.334701977233478	0.0
30	0.0	0.0	0.0	5.535317532629284	0.0
31	1.671796294965051E-4	0.0	0.0	5.718546406557453	0.0
32	1.671796294965051E-4	0.0	0.0	5.904951693446057	0.0
33	1.671796294965051E-4	0.0	0.0	6.087010309967751	0.0
34	3.343592589930102E-4	0.0	0.0	6.266728411676494	0.0
35	3.343592589930102E-4	0.0	0.0	6.483393211503965	0.0
36	3.343592589930102E-4	0.0	0.0	6.689525694673155	0.0
37	3.343592589930102E-4	0.0	0.0	6.88880381303299	0.0
38	3.343592589930102E-4	0.0	0.0	7.103796816565495	0.0
39	3.343592589930102E-4	0.0	0.0	7.317118023803036	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCACTA	50	2.1827873E-11	45.0	8
CCGTAGG	20	7.0305704E-4	45.0	2
GTTGATC	100	0.0	42.75	16
CGTTTTT	2420	0.0	42.303722	1
GCGATAT	75	0.0	42.0	9
TACGAAT	120	0.0	41.249996	12
CGAATAT	115	0.0	41.08696	14
CGAATGG	50	1.0804797E-9	40.5	2
TATAGCG	45	1.9255822E-8	40.0	1
TGCGTAG	45	1.9255822E-8	40.0	1
CTTACGG	45	1.9255822E-8	40.0	2
GGATGCC	330	0.0	39.545456	8
CGAGGGT	80	0.0	39.375	4
GTCCACG	40	3.4545155E-7	39.375	40
AACGGGA	155	0.0	39.193546	4
CACGTGA	230	0.0	39.130436	43
CGTTAGG	110	0.0	38.863636	2
AGGGCGA	715	0.0	38.70629	6
ACGACCA	250	0.0	38.7	28
TGTACGG	70	0.0	38.571426	2
>>END_MODULE