FastQCFastQC Report
Thu 26 May 2016
SRR1545335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545335_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences167415
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4990.2980617029537377No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.2604306663082758No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCT351.2036253E-745.0000048
CGACAAT351.2036253E-745.00000420
TATGCCC351.2036253E-745.00000410
TCATCGA351.2036253E-745.00000416
TTGTTAG351.2036253E-745.0000041
TTCATCG351.2036253E-745.00000415
TCGACAA351.2036253E-745.00000419
ACACGTG253.8764912E-545.042
ACGTTAG253.8764912E-545.01
GTTTAAG207.016009E-445.01
GGATCGC207.016009E-445.08
CACGAGG207.016009E-445.02
TACGGGA253.8764912E-545.04
ACGGGAA207.016009E-445.05
ATGTATG207.016009E-445.01
TACATTG207.016009E-445.01
TCTACGG253.8764912E-545.02
CTAAAGG207.016009E-445.02
CTACGAA253.8764912E-545.011
TACGAAT253.8764912E-545.012