##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545335_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	167415
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231233760415734	31.0	31.0	33.0	30.0	34.0
2	31.598488785353762	31.0	31.0	34.0	30.0	34.0
3	31.79228265089747	31.0	31.0	34.0	30.0	34.0
4	35.56381447301616	37.0	35.0	37.0	33.0	37.0
5	35.47673744885464	37.0	35.0	37.0	33.0	37.0
6	35.523214765702	37.0	35.0	37.0	33.0	37.0
7	35.834017262491415	37.0	35.0	37.0	35.0	37.0
8	35.877113759221096	37.0	35.0	37.0	35.0	37.0
9	37.80774124182421	39.0	37.0	39.0	35.0	39.0
10	37.21675477107786	39.0	37.0	39.0	34.0	39.0
11	36.79649374309351	39.0	35.0	39.0	32.0	39.0
12	35.66204939820207	37.0	35.0	39.0	31.0	39.0
13	35.09242302063734	37.0	35.0	39.0	30.0	39.0
14	35.951438043186094	38.0	35.0	40.0	30.0	41.0
15	36.35654511244512	38.0	35.0	40.0	31.0	41.0
16	36.563414269928025	38.0	35.0	40.0	32.0	41.0
17	36.52644625630917	38.0	35.0	40.0	32.0	41.0
18	36.498491771943975	37.0	35.0	40.0	31.0	41.0
19	36.44903383806708	37.0	35.0	40.0	31.0	41.0
20	36.27203058268375	37.0	35.0	40.0	31.0	41.0
21	36.104082668817014	36.0	35.0	40.0	31.0	41.0
22	35.94791386673835	36.0	34.0	40.0	31.0	41.0
23	35.92371651285727	36.0	34.0	40.0	31.0	41.0
24	35.796314547680915	36.0	34.0	40.0	30.0	41.0
25	35.68621688618105	35.0	34.0	40.0	30.0	41.0
26	35.51497774990293	35.0	34.0	40.0	30.0	41.0
27	35.390645999462414	35.0	34.0	40.0	30.0	41.0
28	35.35999761072783	36.0	34.0	40.0	29.0	41.0
29	35.50251172236658	36.0	34.0	40.0	30.0	41.0
30	35.51147746617686	36.0	34.0	40.0	30.0	41.0
31	35.35431711614849	36.0	34.0	40.0	30.0	41.0
32	35.20936594689842	35.0	34.0	40.0	29.0	41.0
33	35.04573664247528	35.0	34.0	40.0	29.0	41.0
34	34.89640713197742	35.0	34.0	40.0	28.0	41.0
35	34.78218797598782	35.0	34.0	40.0	27.0	41.0
36	34.54863064838874	35.0	33.0	40.0	26.0	41.0
37	34.38675745900905	35.0	33.0	40.0	25.0	41.0
38	34.38542543977541	35.0	33.0	40.0	26.0	41.0
39	34.35328972911627	35.0	33.0	40.0	26.0	41.0
40	34.154149867096734	35.0	33.0	40.0	24.0	41.0
41	34.12949257832333	35.0	33.0	39.0	24.0	41.0
42	34.07756772093301	35.0	33.0	39.0	24.0	41.0
43	33.93276588119344	35.0	33.0	39.0	24.0	41.0
44	33.81499268285398	35.0	33.0	39.0	23.0	41.0
45	33.769692082549355	35.0	33.0	39.0	23.0	41.0
46	33.811313203715315	35.0	33.0	39.0	24.0	41.0
47	33.812633276588116	35.0	33.0	39.0	24.0	41.0
48	33.80374518412329	35.0	33.0	39.0	24.0	41.0
49	33.76568407848759	35.0	33.0	39.0	24.0	41.0
50	33.598118448167725	35.0	33.0	39.0	24.0	41.0
51	33.35456798972613	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	4.0
16	15.0
17	40.0
18	87.0
19	202.0
20	293.0
21	478.0
22	702.0
23	1055.0
24	1436.0
25	2022.0
26	2654.0
27	3126.0
28	3544.0
29	4074.0
30	4886.0
31	6133.0
32	7431.0
33	10033.0
34	18962.0
35	28912.0
36	11512.0
37	13712.0
38	18289.0
39	27787.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.215751276767314	24.29232744975062	29.74046531075471	13.751455962727356
2	28.8743541498671	26.44267240091987	30.606576471642327	14.076396977570708
3	28.230445300600305	25.424842457366424	31.489412537705704	14.855299704327567
4	25.682286533464744	28.83074993280172	30.315682585192487	15.17128094854105
5	23.356927395991995	32.1542275184422	29.52722276976376	14.961622315802048
6	21.869605471433264	40.72335214885165	26.683391571842428	10.723650807872652
7	88.391721171938	4.209300241913807	5.518621389959084	1.880357196189111
8	90.59940865513842	2.7034614580533405	4.698503718304811	1.9986261685034197
9	86.87154675506974	3.8329898754591882	6.605142908341547	2.6903204611295286
10	59.58187737060599	18.70501448496252	10.634053101573933	11.079055042857568
11	51.629184959531706	18.585550876564227	16.348594809306217	13.436669354597855
12	44.87829644894424	19.759878147119434	21.381596631126243	13.980228772810083
13	22.29071469103724	41.56019472568169	21.66532270107219	14.48376788220888
14	16.645461876175972	42.532031180001795	26.088462802018935	14.734044141803304
15	14.688050652569961	21.161783591673387	49.5385718125616	14.611593943195054
16	16.995490248782964	15.655108562554132	47.25383030194427	20.095570886718633
17	17.417196786428935	16.581548845682885	27.286682794253803	38.71457157363438
18	23.766687572798137	21.877370605979156	31.944568885703195	22.411372935519516
19	32.16617387928202	22.536212406295732	23.09709404772571	22.200519666696533
20	33.96410118567631	22.16288863005107	21.87318937968521	21.999820804587404
21	24.127467670160975	26.799271271988772	25.824448227458713	23.24881283039154
22	25.08138458322134	24.133440850580893	22.877281008272853	27.90789355792492
23	22.024908162351046	29.57918943941702	22.47409132992862	25.92181106830332
24	21.98966639787355	22.750649583370667	36.9435235791297	18.316160439626078
25	18.588537466774184	23.4405519218708	34.235283576740436	23.73562703461458
26	18.283307947316548	33.6296030821611	25.4475405429621	22.639548427560253
27	20.73231191948153	30.89627572200818	27.166621867813518	21.20479049069677
28	16.695636591703252	27.4897709285309	37.46199563957829	18.35259684018756
29	17.59280829077442	23.161604396260792	36.43998446973091	22.80560284323388
30	19.99581877370606	27.99032344771974	30.267299823791177	21.746557954783025
31	30.097661499865602	25.150076158050354	22.462742287130784	22.28952005495326
32	32.115401845712746	25.246244362810977	23.175940029268585	19.462413762207685
33	28.98306603350954	26.106979661320672	22.87967028044082	22.030284024728967
34	20.210255950780994	25.23847922826509	28.492667921034553	26.058596899919362
35	21.677269061912018	23.78460711405788	29.910103634680286	24.62802018934982
36	31.85019263506854	23.380820117671654	23.90645999462414	20.862527252635665
37	22.972254576949496	29.652659558581966	27.51545560433653	19.859630260132008
38	22.910730818624376	31.32395544007407	21.397126900218023	24.368186841083535
39	22.53382313412777	30.34196457904011	25.216378460711407	21.90783382612072
40	24.96072633873906	24.666845862079263	23.32945076606039	27.042977033121286
41	18.768927515455605	22.480064510348534	25.47800376310366	33.27300421109219
42	23.68664695517128	25.352566974285455	22.218439207956276	28.742346862586988
43	24.294119403876593	25.43917809037422	24.388495654511246	25.87820685123794
44	21.63067825463668	29.386853029895764	26.333363199235432	22.64910551623212
45	18.745632111817937	34.455693934235285	21.414449123435773	25.384224830511005
46	23.05050324045038	30.4267837410029	25.150673476092344	21.37203954245438
47	23.266135053609293	25.26834513036466	26.69712988680823	24.76838992921781
48	25.522802616253028	21.029776304393273	28.914374458680527	24.533046620673176
49	21.556610817429743	21.685631514499896	30.752322073888244	26.005435594182124
50	19.57709882627005	31.64770181883344	25.645252814861273	23.12994654003524
51	19.52871606486874	33.06334557835319	22.353433085446344	25.054505271331724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42.0
1	128.5
2	215.0
3	512.0
4	809.0
5	598.5
6	388.0
7	406.0
8	424.0
9	445.0
10	466.0
11	485.0
12	504.0
13	543.5
14	583.0
15	549.5
16	516.0
17	474.5
18	433.0
19	430.5
20	428.0
21	423.5
22	419.0
23	419.0
24	419.0
25	451.5
26	542.5
27	601.0
28	917.0
29	1233.0
30	1385.5
31	1538.0
32	1775.5
33	2013.0
34	2414.5
35	2816.0
36	2868.5
37	2921.0
38	3870.5
39	4820.0
40	7889.5
41	10959.0
42	13397.0
43	15835.0
44	15810.0
45	15785.0
46	14937.0
47	14089.0
48	13882.5
49	13676.0
50	13338.5
51	13001.0
52	11982.0
53	10963.0
54	9897.0
55	8831.0
56	8403.0
57	7975.0
58	7672.0
59	7369.0
60	7284.5
61	7200.0
62	6508.5
63	5817.0
64	4938.0
65	4059.0
66	3584.0
67	3109.0
68	2697.0
69	2285.0
70	1985.0
71	1685.0
72	1420.5
73	1156.0
74	928.5
75	548.5
76	396.0
77	315.5
78	235.0
79	153.0
80	71.0
81	60.0
82	49.0
83	54.0
84	59.0
85	38.0
86	17.0
87	17.0
88	17.0
89	10.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	167415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.276767314756743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.83018219319354	20.58955290744557
2	5.981436919903747	3.7416002150344947
3	3.724074710668042	3.4943105456500314
4	3.0021771513693136	3.75593584804229
5	2.7710935411176045	4.33354239464803
6	2.471257782361254	4.637577278021682
7	2.3643099957984797	5.176358151897978
8	2.173331805507811	5.437983454290237
9	1.9938123066345823	5.612400322551743
>10	9.657767082999122	42.103156825851926
>50	0.024827164737786945	0.4969686109368933
>100	0.005729345708720065	0.6206134456291252
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	499	0.2980617029537377	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	436	0.2604306663082758	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04838276140130813	0.0
2	0.0	0.0	0.0	0.1475375563718902	0.0
3	0.0	0.0	0.0	0.22996744616671147	0.0
4	0.0	0.0	0.0	0.41394140310008065	0.0
5	0.0	0.0	0.0	0.7018486993399635	0.0
6	0.0	0.0	0.0	1.1504345488755487	0.0
7	0.0	0.0	0.0	1.3750261326643372	0.0
8	0.0	0.0	0.0	1.9036525998267777	0.0
9	0.0	0.0	0.0	2.134217364035481	0.0
10	0.0	0.0	0.0	2.453782516500911	0.0
11	0.0	0.0	0.0	2.72795149777499	0.0
12	0.0	0.0	0.0	2.9734492130334798	0.0
13	0.0	0.0	0.0	3.083355732759908	0.0
14	0.0	0.0	0.0	3.1257653137413017	0.0
15	0.0	0.0	0.0	3.179523937520533	0.0
16	0.0	0.0	0.0	3.29719559179285	0.0
17	0.0	0.0	0.0	3.4244243347370307	0.0
18	0.0	0.0	0.0	3.5880894782426904	0.0
19	0.0	0.0	0.0	3.677687184541409	0.0
20	0.0	0.0	0.0	3.766687572798136	0.0
21	0.0	0.0	0.0	3.8921243616163426	0.0
22	0.0	0.0	0.0	4.008601379804677	0.0
23	0.0	0.0	0.0	4.150165755756652	0.0
24	0.0	0.0	0.0	4.274407908490876	0.0
25	0.0	0.0	0.0	4.359824388495655	0.0
26	0.0	0.0	0.0	4.451214048920348	0.0
27	0.0	0.0	0.0	4.521100259833348	0.0
28	0.0	0.0	0.0	4.607114057880119	0.0
29	0.0	0.0	0.0	4.709852761102649	0.0
30	0.0	0.0	0.0	4.846638592718693	0.0
31	0.0	0.0	0.0	4.968491473284951	0.0
32	0.0	0.0	0.0	5.077203356927396	0.0
33	0.0	0.0	0.0	5.184720604485858	0.0
34	0.0	0.0	0.0	5.301197622674193	0.0
35	0.0	0.0	0.0	5.462473494011887	0.0
36	0.0	0.0	0.0	5.595675417375982	0.0
37	0.0	0.0	0.0	5.718722934026222	0.0
38	0.0	0.0	0.0	5.817280410954813	0.0
39	0.0	0.0	0.0	5.948093062150942	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACCT	35	1.2036253E-7	45.000004	8
CGACAAT	35	1.2036253E-7	45.000004	20
TATGCCC	35	1.2036253E-7	45.000004	10
TCATCGA	35	1.2036253E-7	45.000004	16
TTGTTAG	35	1.2036253E-7	45.000004	1
TTCATCG	35	1.2036253E-7	45.000004	15
TCGACAA	35	1.2036253E-7	45.000004	19
ACACGTG	25	3.8764912E-5	45.0	42
ACGTTAG	25	3.8764912E-5	45.0	1
GTTTAAG	20	7.016009E-4	45.0	1
GGATCGC	20	7.016009E-4	45.0	8
CACGAGG	20	7.016009E-4	45.0	2
TACGGGA	25	3.8764912E-5	45.0	4
ACGGGAA	20	7.016009E-4	45.0	5
ATGTATG	20	7.016009E-4	45.0	1
TACATTG	20	7.016009E-4	45.0	1
TCTACGG	25	3.8764912E-5	45.0	2
CTAAAGG	20	7.016009E-4	45.0	2
CTACGAA	25	3.8764912E-5	45.0	11
TACGAAT	25	3.8764912E-5	45.0	12
>>END_MODULE