FastQCFastQC Report
Thu 26 May 2016
SRR1545334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545334_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4983455
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120600.24200078058294897No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC101660.20399501951959031No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA207.0355553E-445.012
ACTACGT207.0355553E-445.014
TACGTTA302.1669657E-644.99999612
TACGTAG2350.044.0425531
TATCACG1550.040.645161
CGTTAGG7750.039.7741932
ACGTTAC403.4613186E-739.37518
ACGTATG2150.038.7209321
AGTCGAC356.252487E-638.57142637
TCTTACG1900.037.8947371
GGGCGAT105700.037.762547
AGGGCGA58800.037.576536
CGACGAA301.1404685E-437.49999645
GGCGATA26500.037.188688
ATACTAT31550.036.9413645
GGTACCT16950.036.9026578
TACGGGA13350.036.7415734
CGTTTTT105050.036.711091
GCGATAA5700.036.7105269
TATAGCG2700.036.6666681
Produced by FastQC (version 0.11.5)