##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545334_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4983455
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203239720234254	31.0	31.0	33.0	30.0	34.0
2	31.567925666028888	31.0	31.0	34.0	30.0	34.0
3	31.753064891726723	31.0	31.0	34.0	30.0	34.0
4	35.54166557137568	37.0	35.0	37.0	33.0	37.0
5	35.44921324663311	37.0	35.0	37.0	33.0	37.0
6	35.501226358018684	37.0	35.0	37.0	33.0	37.0
7	35.84372067170266	37.0	35.0	37.0	35.0	37.0
8	35.886048133273	37.0	35.0	37.0	35.0	37.0
9	37.78421035205495	39.0	37.0	39.0	35.0	39.0
10	37.151622117587095	39.0	37.0	39.0	33.0	39.0
11	36.76020130612196	39.0	35.0	39.0	32.0	39.0
12	35.97581838302944	37.0	35.0	39.0	32.0	39.0
13	35.612683569932905	37.0	35.0	39.0	30.0	39.0
14	36.58203033838973	38.0	35.0	41.0	31.0	41.0
15	36.85795517366967	38.0	35.0	41.0	31.0	41.0
16	36.959007957330805	38.0	35.0	41.0	32.0	41.0
17	36.909979120911096	38.0	35.0	41.0	32.0	41.0
18	36.85740334767746	38.0	35.0	41.0	32.0	41.0
19	36.80720504148226	38.0	35.0	41.0	31.0	41.0
20	36.691901301406354	38.0	35.0	41.0	31.0	41.0
21	36.55983549565512	38.0	35.0	40.0	31.0	41.0
22	36.44044362796494	38.0	35.0	40.0	31.0	41.0
23	36.36558130854999	38.0	35.0	40.0	31.0	41.0
24	36.24689036020191	38.0	35.0	40.0	31.0	41.0
25	36.09708505444516	38.0	35.0	40.0	30.0	41.0
26	35.93693852959443	37.0	34.0	40.0	30.0	41.0
27	35.808425279249036	37.0	34.0	40.0	30.0	41.0
28	35.7331800929275	37.0	34.0	40.0	30.0	41.0
29	35.867530658950464	37.0	35.0	40.0	30.0	41.0
30	35.860696444534966	37.0	35.0	40.0	30.0	41.0
31	35.761887485690146	37.0	34.0	40.0	30.0	41.0
32	35.62366912112179	37.0	34.0	40.0	29.0	41.0
33	35.45843636593488	37.0	34.0	40.0	29.0	41.0
34	35.32224972433784	37.0	34.0	40.0	28.0	41.0
35	35.18988512989482	37.0	34.0	40.0	27.0	41.0
36	35.02147987691271	37.0	34.0	40.0	27.0	41.0
37	34.92304615974259	36.0	34.0	40.0	26.0	41.0
38	34.88086939683412	36.0	33.0	40.0	27.0	41.0
39	34.80279605213652	36.0	33.0	40.0	26.0	41.0
40	34.638559192367545	36.0	33.0	40.0	25.0	41.0
41	34.57336546632808	36.0	33.0	40.0	25.0	41.0
42	34.49356741457483	36.0	33.0	40.0	25.0	41.0
43	34.37832808764201	35.0	33.0	40.0	25.0	41.0
44	34.249887878991586	35.0	33.0	40.0	24.0	41.0
45	34.21619900249927	35.0	33.0	40.0	24.0	41.0
46	34.2085055448479	35.0	33.0	40.0	24.0	41.0
47	34.17068640130191	35.0	33.0	40.0	24.0	41.0
48	34.09265840666767	35.0	33.0	39.0	24.0	41.0
49	34.05935681168988	35.0	33.0	39.0	24.0	41.0
50	33.92857324888055	35.0	33.0	39.0	24.0	41.0
51	33.68012533473263	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	6.0
12	12.0
13	16.0
14	68.0
15	169.0
16	415.0
17	1015.0
18	2329.0
19	4521.0
20	8020.0
21	12828.0
22	19182.0
23	27794.0
24	39835.0
25	54906.0
26	72862.0
27	88180.0
28	101516.0
29	119414.0
30	141849.0
31	173746.0
32	214917.0
33	281839.0
34	480256.0
35	678662.0
36	374745.0
37	467015.0
38	638431.0
39	978313.0
40	587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.624142286827116	24.105244253233952	29.03435467963491	13.236258780304025
2	30.237375475448257	26.25574024446895	29.771393541227926	13.735490738854871
3	29.043103629911375	25.695506430779446	30.79845207792586	14.462937861383319
4	26.718752351531215	28.129420251612586	29.91733646636721	15.23449093048899
5	23.445882424944138	33.29260523070842	28.61111417681107	14.650398167536377
6	22.870779409064596	41.776398101317255	24.936595193495275	10.416227296122871
7	88.61189676640002	4.371806307070095	5.155981141597546	1.8603157849323413
8	90.11105748923187	2.9627637853657753	4.973557501773368	1.9526212236289884
9	85.48260192978566	4.69511613930496	6.778510089887438	3.0437718410219414
10	53.10903780610039	21.486619223008937	12.562930737811417	12.841412233079257
11	42.66385469518637	21.799715257788023	18.908267457015263	16.628162590010344
12	38.10912308829918	21.208559122135146	24.198954339910763	16.48336344965491
13	23.107382328123762	34.51027449831493	24.995831205458863	17.38651196810245
14	18.317552782156156	36.484166105643574	27.713423719086457	17.484857393113813
15	17.75856308524909	21.95129684124769	42.784012296689745	17.506127776813475
16	19.458749000442463	18.091123527753336	41.210926957301716	21.23920051450249
17	20.510308611194443	19.409024461944576	27.089438953497126	32.99122797336386
18	23.992190157230276	22.37166784891205	31.393260298327164	22.24288169553051
19	30.433243603082598	23.70843119883695	24.21310115171101	21.645224046369435
20	32.07981209823305	22.95923209901564	23.19433003809606	21.766625764655245
21	25.50814645662497	25.698375925938933	25.73866925656999	23.054808360866105
22	24.814731948015986	24.246792636835288	23.839605253784775	27.09887016136395
23	23.1770328015403	27.6750567628282	24.427911960677882	24.719998474953623
24	23.393910449677985	23.041785267449992	33.51672684914382	20.047577433728208
25	21.772164090977043	24.1833426809312	30.92635530971986	23.118137918371893
26	20.90124221047446	29.859806098379536	25.697452871551967	23.541498819594036
27	22.040652519185986	28.451385635066433	27.319500226248657	22.188461619498923
28	19.643841471428956	26.3989942720462	33.636222259456545	20.3209419970683
29	19.569013866885523	24.527782432067713	32.59064243582013	23.312561265226638
30	21.95844047954682	27.428460776710136	28.70578745067428	21.907311293068766
31	28.865977519612397	25.171291804581358	22.758006242656954	23.20472443314929
32	29.639717826287182	25.17141220297966	24.14812614942846	21.040743821304698
33	27.46923570093439	25.79535683576956	24.174132203461255	22.561275259834794
34	21.79491938825574	25.76110349145322	27.050630536445098	25.393346583845943
35	22.867247722714463	24.764445550326027	28.76682943861237	23.60147728834714
36	29.393543234563168	25.03813117606159	24.31082451833116	21.257501071044086
37	23.448852252102206	29.872187067004717	26.039705385119362	20.639255295773715
38	23.369489641222806	29.548977566768436	23.805030847072963	23.27650194493579
39	22.97488389079464	28.584907458781107	25.943306400880513	22.49690224954374
40	25.211464736814115	24.41639384724052	25.211545002412983	25.160596413532378
41	20.158745288158517	23.811873489376346	26.29468912631899	29.734692096146148
42	22.96994755646434	25.77358479207698	24.291881836998627	26.964585814460047
43	23.122733123907008	25.201872997749554	26.083991929294033	25.591401949049402
44	21.841192506002365	27.750225496166813	27.07599446568696	23.332587532143865
45	20.165126403268417	31.585998067605708	23.49283780028113	24.75603772884475
46	22.843288441452767	29.040374599549914	25.653086061778424	22.4632508972189
47	23.586588019757375	25.403740978899176	27.174821484291524	23.834849517051925
48	24.052730485175445	22.93302538098568	29.233754493619386	23.78048964021949
49	21.47355599679339	23.390298497728985	30.26328119748247	24.872864307995158
50	20.963106920800932	28.973513355693992	26.50049012181308	23.562889601692
51	20.65538868114591	29.938506518068287	24.531976309608496	24.874128491177306
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1541.0
1	3991.5
2	6442.0
3	15519.5
4	24597.0
5	18534.5
6	12472.0
7	12535.0
8	12598.0
9	13173.5
10	13749.0
11	13815.0
12	13881.0
13	13979.0
14	14077.0
15	13948.0
16	13819.0
17	13566.0
18	13313.0
19	13323.5
20	13334.0
21	13267.5
22	13201.0
23	14949.0
24	16697.0
25	18594.0
26	23335.0
27	26179.0
28	35902.5
29	45626.0
30	55098.0
31	64570.0
32	74195.0
33	83820.0
34	92035.0
35	100250.0
36	109830.5
37	119411.0
38	142679.0
39	165947.0
40	214623.0
41	263299.0
42	321515.5
43	379732.0
44	393530.0
45	407328.0
46	396645.0
47	385962.0
48	382812.5
49	379663.0
50	370961.0
51	362259.0
52	341376.0
53	320493.0
54	303736.0
55	286979.0
56	275845.5
57	264712.0
58	259706.5
59	254701.0
60	243023.5
61	231346.0
62	210983.5
63	190621.0
64	168180.5
65	145740.0
66	126816.5
67	107893.0
68	91975.0
69	76057.0
70	64296.5
71	52536.0
72	44355.0
73	36174.0
74	28319.5
75	16047.0
76	11629.0
77	8352.5
78	5076.0
79	3914.0
80	2752.0
81	1893.0
82	1034.0
83	769.0
84	504.0
85	398.5
86	293.0
87	189.5
88	86.0
89	70.0
90	54.0
91	40.5
92	27.0
93	24.5
94	22.0
95	12.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4983455.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.660015515784249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.26111689982773	12.412303204496915
2	8.985954748940241	2.8144038158507865
3	3.018093169531749	1.4179015758884896
4	1.3679353459522399	0.8568755496880709
5	0.7897413650770185	0.6183681015281371
6	0.4748239657765786	0.4461450402796458
7	0.36604026223571406	0.4012537330209113
8	0.25569526900958006	0.3203353504002121
9	0.20860041592603162	0.2940017175000632
>10	2.0968581783188682	7.707292521357447
>50	1.0481211811010895	12.196118365561672
>100	2.109710243598695	57.826181625002945
>500	0.013821329502864655	1.3940093594230278
>1k	0.0032292825941272556	0.8452170397035697
>5k	0.0	0.0
>10k+	2.583426075301805E-4	0.44959300029799776
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12060	0.24200078058294897	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10166	0.20399501951959031	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.041316717016607954	0.0
2	0.0	0.0	0.0	0.1489328186970686	0.0
3	0.0	0.0	0.0	0.22303803285070298	0.0
4	0.0	0.0	0.0	0.37578346749393743	0.0
5	0.0	0.0	0.0	0.643609704512231	0.0
6	0.0	0.0	0.0	1.0217208743733013	0.0
7	0.0	0.0	0.0	1.2278027994634244	0.0
8	0.0	0.0	0.0	1.7131889422097721	0.0
9	0.0	0.0	0.0	1.9312304415310262	0.0
10	0.0	0.0	0.0	2.2221731710229147	0.0
11	0.0	0.0	0.0	2.549877544795729	0.0
12	0.0	0.0	0.0	2.8178442466120392	0.0
13	0.0	0.0	0.0	2.9329651817865314	0.0
14	0.0	0.0	0.0	2.9786764403410886	0.0
15	0.0	0.0	0.0	3.0472433281729243	0.0
16	2.0066399716662436E-5	0.0	0.0	3.196818271660926	0.0
17	2.0066399716662436E-5	0.0	0.0	3.365576693278057	0.0
18	2.0066399716662436E-5	0.0	0.0	3.579464447857962	0.0
19	2.0066399716662436E-5	0.0	0.0	3.6929198718559713	2.0066399716662436E-5
20	2.0066399716662436E-5	0.0	0.0	3.815766370921379	2.0066399716662436E-5
21	2.0066399716662436E-5	0.0	0.0	3.9770801582436284	2.0066399716662436E-5
22	2.0066399716662436E-5	0.0	0.0	4.149992324602108	2.0066399716662436E-5
23	2.0066399716662436E-5	0.0	0.0	4.3510777161627825	2.0066399716662436E-5
24	2.0066399716662436E-5	0.0	0.0	4.510705925908832	2.0066399716662436E-5
25	2.0066399716662436E-5	0.0	0.0	4.6364620529331555	2.0066399716662436E-5
26	4.013279943332487E-5	0.0	0.0	4.756338724840497	2.0066399716662436E-5
27	4.013279943332487E-5	0.0	0.0	4.8730649719923225	2.0066399716662436E-5
28	4.013279943332487E-5	0.0	0.0	4.996493396649513	2.0066399716662436E-5
29	4.013279943332487E-5	0.0	0.0	5.1355736130857	2.0066399716662436E-5
30	4.013279943332487E-5	0.0	0.0	5.303750109111048	2.0066399716662436E-5
31	4.013279943332487E-5	0.0	0.0	5.461070682889682	2.0066399716662436E-5
32	6.019919914998731E-5	0.0	0.0	5.608438322408851	2.0066399716662436E-5
33	6.019919914998731E-5	0.0	0.0	5.758274129093169	2.0066399716662436E-5
34	8.026559886664974E-5	0.0	0.0	5.916236827662736	2.0066399716662436E-5
35	8.026559886664974E-5	0.0	0.0	6.113248740080928	2.0066399716662436E-5
36	8.026559886664974E-5	0.0	0.0	6.27616783938051	2.0066399716662436E-5
37	8.026559886664974E-5	0.0	0.0	6.444023273010392	2.0066399716662436E-5
38	8.026559886664974E-5	0.0	0.0	6.60738383310374	2.0066399716662436E-5
39	8.026559886664974E-5	0.0	0.0	6.790148601723102	2.0066399716662436E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTA	20	7.0355553E-4	45.0	12
ACTACGT	20	7.0355553E-4	45.0	14
TACGTTA	30	2.1669657E-6	44.999996	12
TACGTAG	235	0.0	44.042553	1
TATCACG	155	0.0	40.64516	1
CGTTAGG	775	0.0	39.774193	2
ACGTTAC	40	3.4613186E-7	39.375	18
ACGTATG	215	0.0	38.720932	1
AGTCGAC	35	6.252487E-6	38.571426	37
TCTTACG	190	0.0	37.894737	1
GGGCGAT	10570	0.0	37.76254	7
AGGGCGA	5880	0.0	37.57653	6
CGACGAA	30	1.1404685E-4	37.499996	45
GGCGATA	2650	0.0	37.18868	8
ATACTAT	3155	0.0	36.94136	45
GGTACCT	1695	0.0	36.902657	8
TACGGGA	1335	0.0	36.741573	4
CGTTTTT	10505	0.0	36.71109	1
GCGATAA	570	0.0	36.710526	9
TATAGCG	270	0.0	36.666668	1
>>END_MODULE