FastQCFastQC Report
Thu 26 May 2016
SRR1545333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545333_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2700930
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC57740.21377821713261727No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56550.20937232730948227No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC207.034981E-445.014
CGACTAA351.2127384E-745.021
TATACGT253.89221E-545.018
TTCGATC253.89221E-545.044
TAATGCG1000.045.01
TACGTAA207.034981E-445.042
CGTAAAA302.1665965E-644.99999635
TATAACG302.1665965E-644.9999961
GCGTAAG2150.042.9069751
CGTAAGG4350.042.4137952
GTATACG2400.041.2499961
TACGGGA5850.039.230774
TCGTAAG1500.039.0000041
CGTTTTT49700.038.978871
TAGTACG1450.038.7931061
AACACGT3850.038.57142641
TATGTCG1000.038.251
AGGGTAC11700.038.0769236
TATACGG3550.038.0281682
CATACGA2900.038.01724218
Produced by FastQC (version 0.11.5)