##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545333_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2700930
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2872088502849	31.0	31.0	33.0	30.0	34.0
2	31.653615236233446	31.0	31.0	34.0	30.0	34.0
3	31.843255471263603	31.0	31.0	34.0	30.0	34.0
4	35.61910675211872	37.0	35.0	37.0	33.0	37.0
5	35.53980925088766	37.0	35.0	37.0	33.0	37.0
6	35.5895828473896	37.0	35.0	37.0	33.0	37.0
7	35.90886213267282	37.0	35.0	37.0	35.0	37.0
8	35.961751692935394	37.0	35.0	37.0	35.0	37.0
9	37.89573072978567	39.0	38.0	39.0	35.0	39.0
10	37.257179564076075	39.0	37.0	39.0	34.0	39.0
11	36.89216899364293	39.0	37.0	39.0	33.0	39.0
12	35.965413394645545	37.0	35.0	39.0	32.0	39.0
13	35.517282935877645	37.0	35.0	39.0	31.0	39.0
14	36.347524371235096	38.0	35.0	41.0	31.0	41.0
15	36.686143291384816	38.0	35.0	41.0	31.0	41.0
16	36.833775403287014	38.0	35.0	41.0	32.0	41.0
17	36.82973901582048	38.0	35.0	41.0	32.0	41.0
18	36.80231957140688	38.0	35.0	40.0	32.0	41.0
19	36.77273050393753	38.0	35.0	40.0	32.0	41.0
20	36.637238284590865	38.0	35.0	40.0	31.0	41.0
21	36.51641249495544	38.0	35.0	40.0	31.0	41.0
22	36.41906972783449	38.0	35.0	40.0	31.0	41.0
23	36.38559940464951	38.0	35.0	40.0	31.0	41.0
24	36.30057980029101	37.0	35.0	40.0	31.0	41.0
25	36.1879619242261	37.0	35.0	40.0	31.0	41.0
26	36.03951342685667	37.0	35.0	40.0	31.0	41.0
27	35.91187887135172	37.0	34.0	40.0	30.0	41.0
28	35.898728586079606	37.0	35.0	40.0	30.0	41.0
29	36.028908931368086	37.0	35.0	40.0	30.0	41.0
30	36.030946747971996	37.0	35.0	40.0	30.0	41.0
31	35.88183884810047	37.0	35.0	40.0	30.0	41.0
32	35.79846904584717	37.0	34.0	40.0	30.0	41.0
33	35.63781586342482	37.0	34.0	40.0	30.0	41.0
34	35.52274401780127	37.0	34.0	40.0	29.0	41.0
35	35.401822335269706	37.0	34.0	40.0	29.0	41.0
36	35.24101254012507	36.0	34.0	40.0	28.0	41.0
37	35.11470678618105	36.0	34.0	40.0	27.0	41.0
38	35.05738652982491	36.0	34.0	40.0	28.0	41.0
39	35.01920671768613	36.0	34.0	40.0	27.0	41.0
40	34.85139488990829	36.0	34.0	40.0	27.0	41.0
41	34.81809339745939	36.0	34.0	40.0	27.0	41.0
42	34.79036554075818	36.0	34.0	40.0	27.0	41.0
43	34.65220683246141	35.0	33.0	40.0	27.0	41.0
44	34.556960010070604	35.0	33.0	40.0	26.0	41.0
45	34.52768120610308	35.0	33.0	40.0	26.0	41.0
46	34.57885617176306	35.0	34.0	40.0	27.0	41.0
47	34.54781612259481	35.0	33.0	40.0	27.0	41.0
48	34.49575035265631	35.0	34.0	40.0	27.0	41.0
49	34.46771519439601	35.0	34.0	40.0	27.0	41.0
50	34.316658336202714	35.0	34.0	39.0	26.0	41.0
51	34.10056313936311	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	6.0
12	4.0
13	4.0
14	23.0
15	85.0
16	173.0
17	433.0
18	1069.0
19	2020.0
20	3567.0
21	5589.0
22	8705.0
23	12987.0
24	18660.0
25	26286.0
26	35136.0
27	42866.0
28	49882.0
29	58609.0
30	71738.0
31	90349.0
32	114418.0
33	153604.0
34	276827.0
35	405191.0
36	192250.0
37	239020.0
38	345462.0
39	545703.0
40	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.983553812945914	23.699207310074677	29.52834764321919	12.788891233760225
2	29.153624862547346	25.484444246981596	31.534360386977816	13.827570503493241
3	28.999677888727216	25.450752148334093	31.209657414298036	14.339912548640655
4	25.484481271265825	28.036454110250915	30.642778598482746	15.836286020000518
5	23.652519687663137	32.704772059994156	28.78301177742481	14.8596964749179
6	21.374193333407383	41.68112464965771	25.868978462973864	11.075703553961043
7	90.1487635740282	3.7524482307945783	4.5411765577041985	1.5576116374730185
8	91.97887394341949	2.2889152995449713	4.023873258470231	1.7083374985653088
9	88.4034388155191	3.1852732207054606	5.865979495951394	2.5453084678240456
10	53.700873402864936	19.58469860381424	13.199453521564793	13.514974471756025
11	47.40892951687011	21.30392124194259	17.130729045180733	14.156420196006561
12	41.31869393134957	21.189812397951815	21.03597649698437	16.45551717371424
13	23.73911948847249	37.11847400710126	22.519502541717113	16.62290396270914
14	18.638765166072428	38.549129373956376	26.484099921138277	16.328005538832922
15	18.21535545164073	22.049627350579247	44.09296057283972	15.642056624940299
16	19.672261036013523	17.800979662560675	41.919561040086194	20.60719826133961
17	20.94771060338476	18.090990880918795	26.331782015824178	34.62951649987227
18	24.348724328286924	22.68785196210194	31.40418300363208	21.55924070597905
19	31.279559262920547	23.79687737186821	23.2368480486351	21.68671531657614
20	31.89149663264135	24.111546763522192	22.071841921116057	21.925114682720395
21	25.25822587034836	26.927317627631965	26.480360468431247	21.33409603358843
22	24.594639624129467	24.34313366136849	24.379454484196184	26.682772230305858
23	22.77600678284887	30.105074918639136	23.57332474369939	23.5455935548126
24	22.552639276101193	24.992317461022683	33.52415649424457	18.930886768631545
25	20.543479468183182	24.48623251990981	31.883129144405814	23.087158867501195
26	19.715542424276084	31.659243297678945	25.450900245471004	23.174314032573964
27	20.824271639768526	30.180197191337797	27.606565146079316	21.388966022814365
28	17.847667284972214	27.61152640016587	34.75362190060461	19.78718441425731
29	18.5726768187254	25.113238773311412	34.0213185828585	22.292765825104684
30	22.206573291421844	27.39330526892589	29.86071464273417	20.5394067969181
31	28.746061541765243	25.17943819351112	24.495414542398358	21.57908572232527
32	31.217247392564783	25.281069853717053	24.42355040671176	19.078132347006402
33	28.386148474784612	25.10205743947455	25.00068494925822	21.51110913648262
34	21.223948788010055	26.018149304128578	28.738730733488094	24.019171174373273
35	21.339279433380355	25.30013736009449	29.756343185495364	23.604240021029792
36	30.955300581651507	24.397596383467917	24.71607927639739	19.93102375848319
37	23.33529562039742	29.46100047020841	26.77459245519136	20.42911145420281
38	23.37109810324592	31.033495869941095	22.978603666144622	22.616802360668363
39	22.38984349835057	29.27332437345655	24.60904207069417	23.727790057498712
40	25.113905210427518	24.18059705360746	24.585124383082864	26.120373352882154
41	19.44859733499202	23.915614251387485	25.505437016138888	31.130351397481608
42	23.50986512053256	25.99841536063504	23.68210209076133	26.80961742807107
43	22.716434709525977	26.26106563294865	25.77934267085781	25.243156986667554
44	22.57918568789269	28.6647562136005	26.705542165106095	22.050515933400717
45	19.93046839421977	31.474603192233786	23.032955315391366	25.56197309815508
46	22.529017782763713	29.386470586057396	26.624162788372896	21.460348842805995
47	23.801616480249397	25.639427900760108	26.885406137885838	23.673549481104654
48	24.61026387207369	23.516677588830515	28.386333596205755	23.486724942890042
49	22.41309474884577	22.57107736964675	30.630190341845214	24.385637539662262
50	20.06283021033496	30.46406237851407	25.938361971617184	23.53474543953379
51	20.040319445524318	31.94847700606828	23.733565845838285	24.277637702569116
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	532.0
1	1297.0
2	2062.0
3	6085.0
4	10108.0
5	7557.5
6	5007.0
7	5259.5
8	5512.0
9	6024.5
10	6537.0
11	6581.5
12	6626.0
13	6520.0
14	6414.0
15	6207.5
16	6001.0
17	5919.5
18	5838.0
19	5587.5
20	5337.0
21	5874.0
22	6411.0
23	7227.5
24	8044.0
25	7888.5
26	11053.5
27	14374.0
28	14935.0
29	15496.0
30	18972.0
31	22448.0
32	28211.5
33	33975.0
34	41260.5
35	48546.0
36	55087.5
37	61629.0
38	77129.5
39	92630.0
40	126127.0
41	159624.0
42	200065.5
43	240507.0
44	240955.0
45	241403.0
46	241720.0
47	242037.0
48	234575.5
49	227114.0
50	216515.0
51	205916.0
52	198927.5
53	191939.0
54	177388.5
55	162838.0
56	155817.0
57	148796.0
58	139560.5
59	130325.0
60	119722.0
61	109119.0
62	100405.5
63	91692.0
64	77633.5
65	63575.0
66	53667.5
67	43760.0
68	36401.5
69	29043.0
70	22908.5
71	16774.0
72	14375.0
73	11976.0
74	9149.0
75	5052.5
76	3783.0
77	2647.0
78	1511.0
79	1188.0
80	865.0
81	598.5
82	332.0
83	286.5
84	241.0
85	179.0
86	117.0
87	80.0
88	43.0
89	26.5
90	10.0
91	6.5
92	3.0
93	4.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2700930.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.17851870406691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98159021673851	12.77805134598452
2	8.069873536797168	2.6111719990905513
3	2.584457834808398	1.254380982609598
4	1.284633806164116	0.8313388824361128
5	0.7776329785479272	0.629047484416845
6	0.5571940532400951	0.5408744647283839
7	0.40579361462216457	0.4595597708908926
8	0.3258818253132291	0.42178281649164334
9	0.24811379606683184	0.3612702321363853
>10	3.0387598527958675	12.163924173602013
>50	1.766881326408197	21.251428556022834
>100	1.9520267245362797	45.42716941288183
>500	0.005774543517126628	0.6413714261666271
>1k	9.239269627402605E-4	0.20153330427760996
>5k	4.6196348137013025E-4	0.4270951482641952
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5774	0.21377821713261727	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5655	0.20937232730948227	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0347287786058876	0.0
2	0.0	0.0	0.0	0.12543827496454923	0.0
3	0.0	0.0	0.0	0.19485880789209642	0.0
4	0.0	0.0	0.0	0.3553220557363575	0.0
5	0.0	0.0	0.0	0.5936473733121554	0.0
6	0.0	0.0	0.0	0.9400836008337868	0.0
7	0.0	0.0	0.0	1.1437171640879253	0.0
8	0.0	0.0	0.0	1.696526751896569	0.0
9	0.0	0.0	0.0	1.946736864709563	0.0
10	0.0	0.0	0.0	2.3052800331737586	0.0
11	0.0	0.0	0.0	2.6435338938809965	0.0
12	0.0	0.0	0.0	2.919216714242872	0.0
13	0.0	0.0	0.0	3.0348805781712227	0.0
14	0.0	0.0	0.0	3.080642593477062	0.0
15	0.0	0.0	0.0	3.1383264283043246	0.0
16	0.0	0.0	0.0	3.2714657543883034	0.0
17	0.0	0.0	0.0	3.4186002599104754	0.0
18	0.0	0.0	0.0	3.6230853817018582	0.0
19	0.0	0.0	0.0	3.722680706275246	0.0
20	0.0	0.0	0.0	3.8394552987304373	0.0
21	0.0	0.0	0.0	3.99951127944819	0.0
22	0.0	0.0	0.0	4.155050297490123	0.0
23	0.0	0.0	0.0	4.342171029978563	0.0
24	0.0	0.0	0.0	4.472422461892755	0.0
25	0.0	0.0	0.0	4.585272480219776	0.0
26	0.0	0.0	0.0	4.701898975538056	0.0
27	0.0	0.0	0.0	4.81523030956004	0.0
28	0.0	0.0	0.0	4.929265104982358	0.0
29	0.0	0.0	0.0	5.0665141266156475	0.0
30	0.0	0.0	0.0	5.233493648484041	0.0
31	0.0	0.0	0.0	5.386922282324977	0.0
32	0.0	0.0	0.0	5.529095533760594	0.0
33	0.0	0.0	0.0	5.671194736627754	0.0
34	0.0	0.0	0.0	5.826992924659284	0.0
35	0.0	0.0	0.0	6.023332703920501	0.0
36	0.0	0.0	0.0	6.187091113061057	0.0
37	0.0	0.0	0.0	6.348739138000615	0.0
38	0.0	0.0	0.0	6.564109399355037	0.0
39	0.0	0.0	0.0	6.966600393197899	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGTC	20	7.034981E-4	45.0	14
CGACTAA	35	1.2127384E-7	45.0	21
TATACGT	25	3.89221E-5	45.0	18
TTCGATC	25	3.89221E-5	45.0	44
TAATGCG	100	0.0	45.0	1
TACGTAA	20	7.034981E-4	45.0	42
CGTAAAA	30	2.1665965E-6	44.999996	35
TATAACG	30	2.1665965E-6	44.999996	1
GCGTAAG	215	0.0	42.906975	1
CGTAAGG	435	0.0	42.413795	2
GTATACG	240	0.0	41.249996	1
TACGGGA	585	0.0	39.23077	4
TCGTAAG	150	0.0	39.000004	1
CGTTTTT	4970	0.0	38.97887	1
TAGTACG	145	0.0	38.793106	1
AACACGT	385	0.0	38.571426	41
TATGTCG	100	0.0	38.25	1
AGGGTAC	1170	0.0	38.076923	6
TATACGG	355	0.0	38.028168	2
CATACGA	290	0.0	38.017242	18
>>END_MODULE